FastQCFastQC Report
Wed 25 May 2016
SRR1294875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609342
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96240.36882861656310284No Hit
CCCATGTACTCTGCGTTGATACCAC86270.33061975011324696No Hit
GAGTACATGGGAAGCAGTGGTATCA42990.16475417940614914No Hit
CATGTACTCTGCGTTGATACCACTG31700.12148656634507857No Hit
GCGTTGATACCACTGCTTCCCATGT28140.10784328002998457No Hit
GTATCAACGCAGAGTACATGGGAAG26870.10297615260858868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC751.477677E-512.6681795
TAGACCG550.003066661812.0923515
GAACCGT550.003081253712.0846896
TACACCG700.00149050410.85843855
TAACCCG803.768359E-410.6887755
TGCTCGG803.779077E-410.68549210
AGACCGT1106.0863804E-610.3583056
AGCACGC856.6075387E-410.05693210
TAAGACT2800.09.8406494
GTCTAGA2450.09.695221
CTGCTCG1701.6705599E-89.4983979
CCGTTCA900.00111576699.4983969
GGAGCGT800.0045363899.4951136
GTATTAC2451.8189894E-129.3074111
ATTATAC4300.09.2799993
TTAGACT3000.09.1846064
CTTATAC3850.09.1307843
TTATACC3350.09.0758854
GTATTAT5550.09.0733291
CACGGGT1054.4878214E-49.0488734
Produced by FastQC (version 0.11.5)