FastQCFastQC Report
Wed 25 May 2016
SRR1294875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609342
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC99930.3829701127717256No Hit
CCCATGTACTCTGCGTTGATACCAC87890.33682821186337397No Hit
GTATCAACGCAGAGTACTTTTTTTT81150.31099794507580836No Hit
GGTATCAACGCAGAGTACTTTTTTT53430.2047642662403012No Hit
TATCAACGCAGAGTACTTTTTTTTT49090.1881317205640349No Hit
GAGTACATGGGAAGCAGTGGTATCA44310.16981292601736378No Hit
CATGTACTCTGCGTTGATACCACTG32410.1242075588405046No Hit
GCGTTGATACCACTGCTTCCCATGT28730.11010438646984566No Hit
GTATCAACGCAGAGTACATGGGAAG27220.10431748693731982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26810.1027462095808062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG707.0163387E-613.6176355
GCACCGT951.0525746E-611.9886926
GCACCGC658.09035E-411.681296
GTTAGAC1104.758749E-711.2654973
TCGACCG600.005934069411.0690719
AAACCGT700.001503768710.8469126
AGCACCG1002.3117145E-510.4855795
GCTCGTT1002.4344306E-510.43655311
AGACCGT750.002669394210.1237856
TTAGACA3050.09.6886124
TAGACTG3700.09.5323445
CCTACAC2700.09.5323443
TATTCCG1401.011229E-69.5323445
TGTACCG1106.589784E-59.5323445
TAGCACG800.0043922769.5323434
TCGCATC800.00456541879.48777414
CTCGCAT800.00456541879.48777413
TGCTCGT800.00456541879.48777410
GCTCGCA800.00456541879.48777412
CTACACT3850.09.4085474
Produced by FastQC (version 0.11.5)