FastQCFastQC Report
Wed 25 May 2016
SRR1294874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3719145
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84920.2283320494360935No Hit
CCCATGTACTCTGCGTTGATACCAC79970.2150225387824352No Hit
GAGTACATGGGAAGCAGTGGTATCA37490.10080273826376762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG1058.913048E-1114.4732249
CGCAATT400.00529095514.24592712
TCGTCTA1001.4562647E-712.34231414
CGTCCGC1353.7289283E-1011.96034910
TCTAGAC3150.011.7640913
CGTAATC658.0146093E-411.6946392
CATGCGG1302.6284397E-911.689754510
TAAGCCG752.0704174E-411.4028885
TACGACC1104.9558366E-711.2305714
CCGTTTA600.005891373411.08001613
GTCCGCA1658.0035534E-1110.93626711
TAGACTG3800.010.2525975
TCTATAC4000.010.2143993
TAACGCT750.002642604810.1363114
TCGACCG750.002653560410.1312569
GTCGTAC856.585457E-410.0608291
TACCCCG951.6410195E-410.0025335
GACGGAT1054.1170373E-59.9504767
AACACCG1252.5642748E-69.8825035
CTAGACT3800.09.502794
Produced by FastQC (version 0.11.5)