FastQCFastQC Report
Wed 25 May 2016
SRR1294874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3719145
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC84880.22822449783485182No Hit
GTACATGGGAAGCAGTGGTATCAAC82270.221206755853832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATC1052.6061025E-711.8019422
CGGACTG2300.011.6034885
TCGTCCG1001.9623294E-611.3851349
CGTCCGC1251.8704668E-811.38513410
TCGTCTA1354.833055E-911.24487814
CGTATCC1201.220069E-711.1200093
ACCGTCT1553.2014214E-1011.017878
TAACCCG909.208644E-510.5904865
GCGGGAC1909.094947E-1210.5347453
CTAGTAC1855.638867E-1110.30425553
GTCGTAT1303.6612983E-710.2675361
ACCGGAC1502.3259418E-810.1668663
CCTAGTA1701.4733814E-910.0930632
AACCGAC951.6652176E-49.9880327
GTATAGG4700.09.9398491
GTCCTAA3850.09.9056011
TACACCG1201.6365888E-59.5314375
AGACCGT2303.6379788E-129.4927166
CGAACCA800.0045668379.48761110
ATCGTCC1502.7021088E-79.4876118
Produced by FastQC (version 0.11.5)