FastQCFastQC Report
Wed 25 May 2016
SRR1294873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868196
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC37630.2014242616941691No Hit
CCCATGTACTCTGCGTTGATACCAC34390.18408132765512827No Hit
GTATCAACGCAGAGTACTTTTTTTT19730.10560990388588777No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG500.001498849213.3010525
AACGCTA550.00306702312.0918665
CTCGGGC1104.9645314E-711.2281615
TATACTG4300.010.8264375
GTATTAG2600.010.59699251
CTAGTAC1303.8374856E-710.2315793
TAACGCT750.002645078810.1346794
AACCGTG750.002652154810.1314157
ATTAGGC856.589463E-410.0596193
CCTAAAC1701.5570549E-910.0596193
GTTAGGC951.6429089E-410.0007913
CTTATAC2201.8189894E-129.9326043
TTAGGCA1554.0547093E-89.8077544
GTCTAGA1304.2341653E-69.5007521
GTCTAGC800.0045132149.50075151
TCTATAC2451.8189894E-129.3068593
GTCTTAA1856.686605E-99.2439741
GTCCTAG1651.0641088E-79.212851
ACTGATC2201.6916601E-109.0664658
GTCTAAG1901.05592335E-89.0007111
Produced by FastQC (version 0.11.5)