FastQCFastQC Report
Wed 25 May 2016
SRR1294873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868196
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68330.36575391447150085No Hit
GGTATCAACGCAGAGTACTTTTTTT43730.2340760819528572No Hit
GTACATGGGAAGCAGTGGTATCAAC41670.22304940166877565No Hit
TATCAACGCAGAGTACTTTTTTTTT40890.21887425088159915No Hit
CCCATGTACTCTGCGTTGATACCAC34720.1858477376035491No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19020.10180944611807327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC400.00516463714.2973744
CGCGTCC655.5230936E-513.13611410
TGTACCG550.002993492412.13110355
TGGACGG907.191975E-611.6497115
GTCCTAC1002.3072993E-510.4869961
GACTGTC1553.7343852E-910.406987
CGGCATG750.002676605710.12021414
GCTCGTC750.002676605710.12021412
GTCTTAG1701.4661055E-910.0944351
GGACGGG856.635597E-410.051996
CACCGCG1054.163038E-59.93976217
ATAAGCC1106.592838E-59.5315823
TGCACCG1002.6651524E-49.5315825
CTTACAC2700.09.5315823
ACCGCGC1201.7266902E-59.48770118
GTGTATA5550.09.2759671
GTATTAA3500.09.2612431
CTAGGGT1554.0597843E-79.2241124
GTATTAG3050.09.0647661
ACCTAAT1909.95351E-99.0318631
Produced by FastQC (version 0.11.5)