FastQCFastQC Report
Wed 25 May 2016
SRR1294872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046690
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC198760.9711289936433949No Hit
CCCATGTACTCTGCGTTGATACCAC187370.9154781623010813No Hit
GAGTACATGGGAAGCAGTGGTATCA90070.44007641606691783No Hit
CATGTACTCTGCGTTGATACCACTG71380.34875823891258567No Hit
GCGTTGATACCACTGCTTCCCATGT66380.3243285500002443No Hit
GTATCAACGCAGAGTACATGGGAAG58460.2856319227630955No Hit
ACGCAGAGTACATGGGAAGCAGTGG57070.27884046924546463No Hit
TATCAACGCAGAGTACATGGGAAGC54850.267993687368385No Hit
GGTATCAACGCAGAGTACATGGGAA53650.26213056202942314No Hit
GTATCAACGCAGAGTACTTTTTTTT49720.24292882654432277No Hit
ACTCTGCGTTGATACCACTGCTTCC48530.23711456058318556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44300.21644704376334473No Hit
GTGGTATCAACGCAGAGTACATGGG43360.21185426224782453No Hit
GGTATCAACGCAGAGTACTTTTTTT36670.17916733848311175No Hit
GCTTCCCATGTACTCTGCGTTGATA34150.1668547752712917No Hit
GTACTCTGCGTTGATACCACTGCTT32480.15869525917456967No Hit
TATCAACGCAGAGTACTTTTTTTTT30650.14975399303265272No Hit
GGTATCAACGCAGAGTACATGGGGG29080.14208307071417753No Hit
ATACCACTGCTTCCCATGTACTCTG28290.13822317986602758No Hit
CAGTGGTATCAACGCAGAGTACATG25500.12459141345294109No Hit
GGGAAGCAGTGGTATCAACGCAGAG25000.12214844456170697No Hit
GATACCACTGCTTCCCATGTACTCT24540.11990091318177154No Hit
CTGCTTCCCATGTACTCTGCGTTGA23010.11242542837459508No Hit
CATGGGAAGCAGTGGTATCAACGCA22320.10905413130469198No Hit
TACCACTGCTTCCCATGTACTCTGC22250.10871211565991919No Hit
GCAGTGGTATCAACGCAGAGTACAT21590.10548739672349014No Hit
GCAGAGTACATGGGAAGCAGTGGTA21170.10343530285485344No Hit
GGTATCAACGCAGAGTACATGGGGA20690.10109005271926867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG655.4642893E-513.15123710
ATCGCGG853.97005E-612.28716716
GTCTTAA802.8638782E-511.8764241
AACTCGG600.005889705411.080049511
CATCGCG951.3688483E-510.9940515
ACTCGGT700.001495441910.85392612
CCTATAC1855.456968E-1210.7848123
AGACGTA803.765887E-410.6893045
GACTGCG803.7786888E-410.685387
TCGCGGT1002.4144067E-510.44409217
CTCATAC1106.0384173E-610.3648791
GTATAGG2200.010.3648791
GCTACGA1106.0401817E-610.3646252
CCTAGTC750.002646086710.1342992
CTAGCCT856.5831235E-410.0607684
GAACAAA7300.09.7614441
CTATACT2350.09.7040034
TACGGCT800.00450927679.5018374
ACGAGCG900.00111210979.5016045
CACTAGA900.00111285739.5009062
Produced by FastQC (version 0.11.5)