FastQCFastQC Report
Wed 25 May 2016
SRR1294872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046690
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC201490.9844676037895332No Hit
CCCATGTACTCTGCGTTGATACCAC194880.952171555047418No Hit
GTATCAACGCAGAGTACTTTTTTTT179630.8776610038647767No Hit
GGTATCAACGCAGAGTACTTTTTTT132840.6490479750230861No Hit
TATCAACGCAGAGTACTTTTTTTTT109610.5355476403363479No Hit
GAGTACATGGGAAGCAGTGGTATCA95670.4674376676487402No Hit
CATGTACTCTGCGTTGATACCACTG74120.36214570843654875No Hit
GCGTTGATACCACTGCTTCCCATGT66220.32354679995504937No Hit
ACGCAGAGTACATGGGAAGCAGTGG60790.2970161577962466No Hit
TATCAACGCAGAGTACATGGGAAGC57200.2794756411571855No Hit
GTATCAACGCAGAGTACATGGGAAG57080.2788893286232893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55070.2690685936805281No Hit
GGTATCAACGCAGAGTACATGGGAA54240.2650132653210794No Hit
ACTCTGCGTTGATACCACTGCTTCC52000.25406876468835043No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46700.2281732944412686No Hit
GTGGTATCAACGCAGAGTACATGGG43670.21336890296038968No Hit
GCTTCCCATGTACTCTGCGTTGATA35790.17486771323453967No Hit
GAGTACTTTTTTTTTTTTTTTTTTT33780.16504697829177845No Hit
GTACTCTGCGTTGATACCACTGCTT32220.15742491535112793No Hit
GGTATCAACGCAGAGTACATGGGGG28390.13871177364427442No Hit
ATACCACTGCTTCCCATGTACTCTG28000.1368062579091118No Hit
GGGAAGCAGTGGTATCAACGCAGAG26620.13006366376930556No Hit
CAGTGGTATCAACGCAGAGTACATG26470.1293307731019353No Hit
GATACCACTGCTTCCCATGTACTCT25240.12332106962949935No Hit
CATGGGAAGCAGTGGTATCAACGCA25190.12307677274037591No Hit
CTGCTTCCCATGTACTCTGCGTTGA23190.11330489717543937No Hit
GCAGTGGTATCAACGCAGAGTACAT22770.11125280330680268No Hit
GCAGAGTACATGGGAAGCAGTGGTA22680.11081306890638055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22060.10778378748125021No Hit
TACCACTGCTTCCCATGTACTCTGC22060.10778378748125021No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21680.10592713112391226No Hit
GGTATCAACGCAGAGTACATGGGGA20900.102116099653587No Hit
ACATGGGAAGCAGTGGTATCAACGC20550.10040602142972312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG400.005173536514.2937574
CGAGGCG550.003096579612.07648212
ACGAGCG700.001454074610.8904825
TAGACTA909.22619E-510.5879685
ATTACCC1303.684072E-710.2621843
TTAGACT750.002581668610.1644494
GTCTATA1053.9570645E-59.9858821
GCTACGA1002.668265E-49.5305752
ACGAACA800.00456117659.48866514
CTACACT3200.09.2313854
TAATACT2600.09.1626644
GCTTTAG1151.0708156E-49.1175441
CAGCGTT1151.1252811E-49.0761149
GTCCTAA1701.6003105E-78.9712731
CGAGTGC850.00748707638.9337826
CGTATCG850.0075096178.93050810
TATCCGC850.0075096178.93050810
GTTCTAG2252.4556357E-108.8965121
CTAGAGT1304.2144497E-58.7961584
TAGGCAG1752.504894E-78.7123855
Produced by FastQC (version 0.11.5)