FastQCFastQC Report
Wed 25 May 2016
SRR1294871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4406751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTG658.016093E-411.6944715
GGTATCA26150.011.4091181
CGCCTAA1002.4179686E-510.443377514
TCGACCG1054.1153977E-59.9509399
GTATTAG7550.09.9420111
CTAGACT3550.09.9033544
GCACCGT1451.6323611E-79.8222516
GTATCAA54450.09.7196681
TCGTCCG1002.761428E-49.4986249
GTCCGCA1002.76269E-49.49819211
GGAACGA1856.7811925E-99.23749115
GTCTCGC3100.09.195041
TAGACTG5450.09.0658985
GTATTAA11250.09.0370221
TATTCCG2054.134563E-98.8065075
CGTCCGC1201.7676794E-48.707170510
TCTATAC5050.08.6545743
GACATCG1107.1599986E-48.6350157
GAACCGT1356.846877E-58.439866
TGCGTTG5550.08.38579112
Produced by FastQC (version 0.11.5)