FastQCFastQC Report
Wed 25 May 2016
SRR1294871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4406751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156550.35525038741694276No Hit
GGTATCAACGCAGAGTACTTTTTTT94890.21532870815709806No Hit
TATCAACGCAGAGTACTTTTTTTTT90690.20579787693926888No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44410.10077719390090341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.013.5293691
ACGGGTC855.1402567E-511.215855
CGAATTA1252.2859422E-710.62551215
GTATCAA73850.010.4728551
ACTGCGT1409.908763E-810.164028
CCGTTCA856.685404E-410.0444439
AACCGTG951.6668178E-49.9871777
AGACCGT2201.8189894E-129.9243766
TTACGCT800.00438900349.5334734
CTAATAC5400.09.5334723
GTCGTAC850.0072081068.9756481
ACCGGGC850.0075270798.9283938
TACATCG1502.5789632E-68.8979075
TAACCCG1502.5789632E-68.8979075
ACCGTCT2151.0713848E-98.8245748
TACGGCC1106.903036E-48.6667934
GTCTCGC3200.08.6425671
GTATTGG6050.08.512031
GACTGTC3850.08.3788527
GTCTAAT3650.08.3608781
Produced by FastQC (version 0.11.5)