FastQCFastQC Report
Wed 25 May 2016
SRR1294870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856632
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC79320.20567168451643816No Hit
CCCATGTACTCTGCGTTGATACCAC70470.18272420080526222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT701.0894715E-412.2169694
GGACCGC658.0562965E-411.6874296
CGCGATC1001.9430645E-611.39450417
GTCTAGA3600.011.085481
TAACGCT700.001489537210.8595284
TCGTCTA1201.5369405E-610.28563514
ATCGTCG1303.8594044E-710.2282299
AGTTCGC750.002654553410.13081710
CGCGCTT750.00265627110.13002712
TAACCCG856.585921E-410.0607715
CCCGTAC856.5874954E-410.060513
TTGCGTG2151.0913936E-119.71548716
TAGCTCG900.00111683389.49764210
GATCGCC1106.866205E-59.49690111
CTTGCGT2151.07320375E-109.27387315
GTTCTAG3900.09.2582031
TCGTCGC1451.7138209E-69.17013610
GTCCTAA2800.09.1624881
TATACTG8000.09.1455215
CGGTACT1252.7491844E-59.12200454
Produced by FastQC (version 0.11.5)