FastQCFastQC Report
Wed 25 May 2016
SRR1294870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3856632
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85690.22218868691645977No Hit
GTACATGGGAAGCAGTGGTATCAAC78050.2023786557804841No Hit
CCCATGTACTCTGCGTTGATACCAC75090.19470356518329984No Hit
GGTATCAACGCAGAGTACTTTTTTT52200.13535125985574978No Hit
TATCAACGCAGAGTACTTTTTTTTT51160.13265460640268503No Hit
GAGTACATGGGAAGCAGTGGTATCA38670.10026883560578245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT2500.011.3904576
CCGTCTG1751.8189894E-1211.3846989
ACCGTCT2350.010.496538
TAGACAG4600.010.1539025
CCGACTA750.002678772210.1197319
GCGGGAC1701.4733814E-910.0929563
TACACCG1252.4636665E-69.9135255
TCTCACG2500.09.87262619
TCGTCCG1252.6172274E-69.8667389
CGGGACT1856.1118044E-109.7898634
GAGACCG2401.8189894E-129.5322355
GCTCGTC2002.728484E-109.487370512
CGTCTGG1951.7262209E-99.24398610
ACTGCGT1651.0892654E-79.1997568
GCTCGTT2401.0913936E-119.09206411
CTAGACA4100.09.0672484
TTAGACA4100.09.0672484
GAGCGCG950.00184716888.987929
CGACCGG850.0075205248.92929112
TTGCGGC850.0075205248.92929111
Produced by FastQC (version 0.11.5)