FastQCFastQC Report
Wed 25 May 2016
SRR1294869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926211
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64970.2220277348420876No Hit
CCCATGTACTCTGCGTTGATACCAC62240.21269826406913242No Hit
GAGTACATGGGAAGCAGTGGTATCA29950.1023507874175854No Hit
ATTCCATTCCATTCCATTCCATTCC29710.10153061416282011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG707.2651346E-613.5719061
ACGGGTC752.0739979E-411.4005965
TACGACC951.3596547E-511.0011414
TCGATCA700.001495699610.85399159
CGACCAT700.001498907710.8512046
GGTACGA909.518984E-510.5557462
ACCGTCT1002.408056E-510.4469678
GCACCGA951.6540523E-49.994536
GTATTAG4300.09.942211
AGCACCG1356.4435153E-79.8523675
GTCCTAA3100.09.8067961
GTCTATA3000.09.5003341
CGGTTTA900.00111954389.494966515
CTTGCGT1701.6809281E-89.494966515
GTACCGT900.00111971919.4948036
GTGTAGA4700.09.2981991
TTGCGTG1752.6959242E-89.22352416
TCGTCTA1151.1178182E-49.08198614
TACGTCG850.0074348318.9416455
GTACGAC850.00743692048.9413383
Produced by FastQC (version 0.11.5)