FastQCFastQC Report
Wed 25 May 2016
SRR1294869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926211
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81150.27732108176751435No Hit
GTACATGGGAAGCAGTGGTATCAAC66770.2281790342528273No Hit
CCCATGTACTCTGCGTTGATACCAC63740.2178243469114155No Hit
GGTATCAACGCAGAGTACTTTTTTT48580.1660167363187412No Hit
TATCAACGCAGAGTACTTTTTTTTT46170.15778082988547307No Hit
GAGTACATGGGAAGCAGTGGTATCA30650.10474295941065084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC752.1031004E-411.38350411
AAGACCG855.127976E-511.2181885
ACGTCTA600.0057169811.1247025
AGACCGT1408.4892235E-910.84662156
ACCGTCT1053.5297217E-610.8414338
ACCGTGT1605.5842975E-1010.6720358
GTCTACG1105.7548823E-610.40714551
GTATTAA4050.09.8932131
TCTATAC3600.09.8003333
TAGGACC1856.0754246E-109.7931754
CCGGACT800.00438071379.53545954
TACGTCT800.00438071379.53545954
AATCGGA800.00457173739.48625417
TAATCGG900.00112898459.48625316
CTAGGAC2800.09.1949073
CCGTCTG1554.353933E-79.1802459
ATTATAC4900.09.1462573
GTATAGA4750.09.0377861
CTAATAC3700.09.020033
GGACCGA950.00184062398.9912796
Produced by FastQC (version 0.11.5)