FastQCFastQC Report
Wed 25 May 2016
SRR1294868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888105
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA64270.1652990338481085No Hit
GTATCAACGCAGAGTACTTTTTTTT42730.10989929541511867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA602.5837142E-514.24208514
TCGTCGC700.001494699610.85502910
CTAGGAC3000.010.4502553
TCGACCG1201.5292535E-610.2896629
GTCGTAT856.591511E-410.0598481
CGTAGGG856.593856E-410.0594592
TATAGCG856.594637E-410.0593295
TAGGACC2950.09.9839374
GTCCGGT1106.834164E-59.5009671
ACGGGTC1401.0590247E-69.5004785
GCACCGT1701.6807462E-89.4953366
GTCTAGA3800.09.2509421
TAGACAG5250.09.2290355
ACCGTCT1054.504061E-49.0458578
CTAATAC4350.08.9542423
GTATACG850.00743273468.9420871
GTATTGG5100.08.9420861
TTCGAAT850.00745319588.9390913
CGGGTCT1502.7223668E-68.8623136
AGCACCG1401.0853641E-58.8218725
Produced by FastQC (version 0.11.5)