FastQCFastQC Report
Wed 25 May 2016
SRR1294868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888105
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT149950.3856634530188871No Hit
GGTATCAACGCAGAGTACTTTTTTT92310.23741642779708882No Hit
TATCAACGCAGAGTACTTTTTTTTT86230.22177899002213158No Hit
GAGTAAGCAGTGGTATCAACGCAGA66670.17147170665401268No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40820.10498687664041995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC1052.6105045E-711.800453
AGTACCG750.002578159610.1665425
GACAGCG1409.87402E-810.1663577
CGCCTAA951.6664E-49.98735714
CGTCCGC1054.1654333E-59.93992610
ATCGTCC1201.7273347E-59.4881118
CCGCCTA1304.311707E-69.48798913
GTCCGCA1201.7275977E-59.48798811
TAGGACA5300.09.1714674
TACGACA1252.6520398E-59.1498884
TAAGACA6200.09.0699494
TCGACCG1054.551924E-49.0362969
GGTATCA49900.09.0362721
GTGCTAG3600.09.003371
TAGGACG3300.08.9534884
ATAAGAC4650.08.8137363
GTCTAAG3300.08.6663461
GTCTTAG4750.08.6298551
GCGCCCC1107.2389323E-48.62544412
CCCTTAG3800.08.5295091
Produced by FastQC (version 0.11.5)