FastQCFastQC Report
Wed 25 May 2016
SRR1294867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787416
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC63950.16884862925012728No Hit
CCCATGTACTCTGCGTTGATACCAC56970.1504191776134441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG350.00216797516.2890835
TCGACCG602.5757574E-514.2467259
TAACGCT802.8624514E-511.8776144
TCGTTAC600.005889522411.08049313
CTGCGCT3000.010.7690374
ATCGACC909.5433614E-510.5532698
GCGCTGA2350.010.5050276
GCTGCGC2400.010.2928423
GTATCGT750.002660832610.1279236
TGCGCTG2850.010.0020695
GCACCGT1901.0004442E-109.9946616
GTATTAG4950.09.78951551
TACATCG1106.82619E-59.50196555
CGCCTAA800.00454039649.494314
GTATTAA6100.09.3458251
CACCGAC1054.5043803E-49.0457797
CCGCCTA950.00182897618.99769413
GTCTAAT2900.08.8463061
TGCTGCG2900.08.8458382
GTGTAGA4350.08.7370921
Produced by FastQC (version 0.11.5)