FastQCFastQC Report
Wed 25 May 2016
SRR1294867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787416
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115930.30609259716915177No Hit
GGTATCAACGCAGAGTACTTTTTTT71560.18894148411476322No Hit
TATCAACGCAGAGTACTTTTTTTTT67200.17742967764829634No Hit
GTACATGGGAAGCAGTGGTATCAAC63810.16847898408835998No Hit
CCCATGTACTCTGCGTTGATACCAC59290.15654472600844482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATC752.1003754E-411.3852758
TAGACCG700.001452067610.8927315
TAGACAG5150.010.3639575
GAACGAT750.00267674710.120645516
GTCTCGC1909.276846E-1110.0355731
GTCGTCC1304.3131004E-69.4877298
TGCGCTG2850.09.3639275
CGTATCC1054.350558E-49.0772753
TTAGACT3300.08.9534944
GACCGGG1951.6849299E-88.7588287
TCGCTCT2303.8016879E-108.7023444
AGGACCG1651.0027234E-68.6646725
TCTACAC3750.08.6415663
TGCACCG1000.00282155288.5780255
ATCGTCC1000.0029405988.5389578
AGGTCGT1451.7082551E-58.5101736
GTGCTAG3250.08.5070781
GTATCAA63600.08.4694871
CTGCGCT3500.08.4418664
GTCTAGG5200.08.4337421
Produced by FastQC (version 0.11.5)