FastQCFastQC Report
Wed 25 May 2016
SRR1294866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948771
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC40180.10175317839398638No Hit
GTACATGGGAAGCAGTGGTATCAAC39750.10066423198509106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC752.0738751E-411.4008763
TATTCCG2100.010.4062225
GTCTTAG4350.09.8284661
TACACCG900.00111288349.5013335
GGACCGG900.00111910189.4955466
TAGACTG4750.09.2012915
AGACCGT1356.894643E-69.143866
AAAGGCG950.0018224239.0012645
TACGACC850.0074296558.9425444
CCGTCCC850.0074482458.939829
CTAGACA3650.08.8506694
CACGGGT1304.3643005E-58.7705734
ACTCGCG1107.159257E-48.6350558
GACCGGG1000.00291077758.5485957
CCGTGTC1451.6937895E-58.515959
GTACTAG4250.08.4948781
ACTATAC4500.08.4450933
GGTATCA27800.08.3388761
GTCCTAT3900.08.2827931
GTATCAA45050.08.2671111
Produced by FastQC (version 0.11.5)