FastQCFastQC Report
Wed 25 May 2016
SRR1294866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3948771
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132220.33483835856776706No Hit
GGTATCAACGCAGAGTACTTTTTTT80700.20436738418105277No Hit
TATCAACGCAGAGTACTTTTTTTTT77610.19654216463806082No Hit
GTACATGGGAAGCAGTGGTATCAAC40430.1023862867712511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG550.002991165512.1329154
ACCGTTC550.003101196612.0745368
CTACACG951.3102041E-511.0382174
TATACGG1053.954923E-59.9869585
GGTATCA33700.09.9323211
TATGTCG900.00107951729.5330055
TAACCCG1701.5756086E-89.5330045
GTATCAA62600.09.4904591
GTCCTAG4150.09.4212671
TCTACAC4850.09.238173
TAGACAG5700.09.1985135
CTGTGCG1356.972574E-69.135769
TAATTCG950.00176805979.0312685
TAAACGG850.00723032568.97223955
TAACACC3400.08.97223954
GTACTAC3500.08.7187751
TTGCGTG1000.00294198288.5385316
CGAATTA1000.00294198288.5385315
TACACTG8500.08.4114745
CTAGAAC3750.08.3890443
Produced by FastQC (version 0.11.5)