FastQCFastQC Report
Wed 25 May 2016
SRR1294865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703754
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC78920.29189046044869466No Hit
CCCATGTACTCTGCGTTGATACCAC70860.2620800560997783No Hit
GAGTACATGGGAAGCAGTGGTATCA36040.13329615046339274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC250.00604079418.9958658
GCGGCTT350.002174453616.28156711
GTCGTAA550.00306891212.0911931
CGTGCGT550.003074458612.08827810
CGAATTA1001.9413746E-611.39498215
AGTACGG1408.361894E-910.8569953
GTATTAG3600.010.8196981
TGGACGG803.771148E-410.6879485
ATTTTCG909.5626885E-510.5509115
GTACGGT750.002647727710.13375954
AGTACCG1106.8365516E-59.50045
GTCTAGG3600.09.5002231
CGCTCAC900.00111629269.49793210
GCGTCTC800.0045253849.4979329
GTGCCGA800.0045267699.4975811
GGACTGT5550.09.4109696
TAGACAG3950.09.3801395
TAAGACC3000.09.183724
AGGACCG1356.847169E-69.1485325
CCGAATT1356.8891713E-69.14412214
Produced by FastQC (version 0.11.5)