FastQCFastQC Report
Wed 25 May 2016
SRR1294865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703754
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93920.3473688804528814No Hit
GTACATGGGAAGCAGTGGTATCAAC82640.305649108609733No Hit
CCCATGTACTCTGCGTTGATACCAC73730.272694927127246No Hit
GGTATCAACGCAGAGTACTTTTTTT56650.20952349954914537No Hit
TATCAACGCAGAGTACTTTTTTTTT55060.20364278702870156No Hit
GAGTACATGGGAAGCAGTGGTATCA37070.13710566863701357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT905.1629286E-712.7155664
GTCGTAC700.001441010910.9025021
GTGTTAG3950.010.1434681
ACACCGC750.002665943710.1253886
AAGACCG800.00437592959.53667455
CGTACCC900.001075499.53667453
GGAGCGT800.00454659349.4925516
ATACCGT900.00112209489.4925516
GGCACGC800.0045730339.4858818
CGACGTA800.00457442839.48552914
GTATTAG3400.09.259111
ATAGAAC2501.8189894E-129.1552073
TAGACTG2304.0017767E-119.1220365
GTAGGAC2002.4792826E-99.059843
CGTTAAT950.00175643899.0376011
GACCGTG1054.556435E-49.0351747
GTCCTAG2651.8189894E-128.9997071
GACTCCG950.0018473568.9876217
TAGGACT3650.08.8834774
TAGGACC2159.695214E-108.87132554
Produced by FastQC (version 0.11.5)