FastQCFastQC Report
Wed 25 May 2016
SRR1294864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012569
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48990.16261868192894502No Hit
CCCATGTACTCTGCGTTGATACCAC43240.14353198217202662No Hit
ATTCCATTCCATTCCATTCCATTCC38390.12743276585532148No Hit
GTATCAACGCAGAGTACTTTTTTTT35750.11866948109736243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT400.00527402814.2526841
TACCGAT500.001503256613.29630857
CGCCTTA855.318058E-511.1782042
TGTAACG600.00587104411.0850522
ATCGACC856.6115946E-410.0563668
TGTGCGC900.00111647299.49783710
TGGTCGT800.0045260149.49783710
TCGACCG800.0045266359.4976799
CTGTGCG1201.7064436E-59.4976799
GTATTAT5200.09.3190621
GTATAAG4100.09.0382871
TATCCCG850.00742563478.9430095
ACTGTCG850.0074530518.93899258
TACCCGC850.0074581388.93824911
CGCAGTA850.00746322758.93750618
CGCCGCA850.00746933938.93661315
GTATTAG4600.08.8821071
CCTAATA2607.2759576E-128.7705912
TACTATC3250.08.7667977
TGCGTTA1304.3887634E-58.76621313
Produced by FastQC (version 0.11.5)