FastQCFastQC Report
Wed 25 May 2016
SRR1294864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012569
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133110.44184880080754996No Hit
GGTATCAACGCAGAGTACTTTTTTT79830.26498978114692145No Hit
TATCAACGCAGAGTACTTTTTTTTT76460.2538033153763449No Hit
GTACATGGGAAGCAGTGGTATCAAC48110.15969758700962533No Hit
CCCATGTACTCTGCGTTGATACCAC45780.1519633243255175No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36370.12072752524506492No Hit
ATTCCATTCCATTCCATTCCATTCC36140.11996405725478818No Hit
GAATGGAATGGAATGGAATGGAATG30870.10247068199931686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC400.00532505514.2320138
TCTACGC550.002995138312.1306113
CGCAAGT658.112756E-411.67754812
TAGACGT600.005736120511.1197274
GGTATCA32550.09.6368941
GGCTACG800.0043848969.5343741
TAACGTG1002.667464E-49.5311965
TGTACCG800.0043969799.5311965
AGCGCAA1002.791952E-49.48800910
ATCGCTC1002.791952E-49.4880098
GTATCAA58000.09.2220421
ATATCGC850.0074880128.9338946
GTATAGG3100.08.9192531
ACCGTCT1502.7500864E-68.8554758
GTCCTAA2850.08.6980251
CTAATAC4500.08.6839783
TGGACCG1106.917864E-48.6647225
CTAGTAC1651.0023123E-68.6647223
TATATCG1000.00282105648.5780755
ATGCGTT1554.2819265E-68.5698157
Produced by FastQC (version 0.11.5)