FastQCFastQC Report
Wed 25 May 2016
SRR1294863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568363
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC162532.8596161256098656No Hit
CCCATGTACTCTGCGTTGATACCAC115332.029160941159083No Hit
GAGTACATGGGAAGCAGTGGTATCA73281.2893168626388416No Hit
CATGTACTCTGCGTTGATACCACTG45250.7961461249236843No Hit
GTATCAACGCAGAGTACATGGGAAG39580.6963859364525841No Hit
GCGTTGATACCACTGCTTCCCATGT37880.6664754743007549No Hit
GCTTCCCATGTACTCTGCGTTGATA34870.6135163619025166No Hit
ACGCAGAGTACATGGGAAGCAGTGG34070.5994408503016557No Hit
TATCAACGCAGAGTACATGGGAAGC26660.46906642409868343No Hit
GGTATCAACGCAGAGTACATGGGAA24350.4284233843511981No Hit
ACTCTGCGTTGATACCACTGCTTCC22340.3930586614540355No Hit
GTGGTATCAACGCAGAGTACATGGG19900.3501283510714103No Hit
CAGTGGTATCAACGCAGAGTACATG18000.31669901101936615No Hit
GCAGTGGTATCAACGCAGAGTACAT15350.270073878841515No Hit
CATGGGAAGCAGTGGTATCAACGCA14250.2507200503903315No Hit
GGGAAGCAGTGGTATCAACGCAGAG13620.23963558500465373No Hit
ACATGGGAAGCAGTGGTATCAACGC12590.22151336381854553No Hit
GTATCAACGCAGAGTACTTTTTTTT12400.21817042981334112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11930.20990106674783543No Hit
ATACCACTGCTTCCCATGTACTCTG11040.1942420600918779No Hit
AAGCAGTGGTATCAACGCAGAGTAC11040.1942420600918779No Hit
GTACTCTGCGTTGATACCACTGCTT10900.19177884556172728No Hit
CTGCTTCCCATGTACTCTGCGTTGA10800.1900194066116197No Hit
GCAGAGTACATGGGAAGCAGTGGTA10380.18262976302116782No Hit
ATGGGAAGCAGTGGTATCAACGCAG10050.17682361448581277No Hit
GATACCACTGCTTCCCATGTACTCT9300.16362782236000586No Hit
GTACATGGTAAGCAGTGGTATCAAC8910.15676601045458624No Hit
TACCACTGCTTCCCATGTACTCTGC8430.1483207034940698No Hit
CCATGTACTCTGCGTTGATACCACT8360.14708909622899452No Hit
GGTATCAACGCAGAGTACTTTTTTT8080.14216266716869325No Hit
GTTGATACCACTGCTTCCCATGTAC7830.1377640697934243No Hit
CCACTGCTTCCCATGTACTCTGCGT7560.13301358462813379No Hit
CTTCCCATGTACTCTGCGTTGATAC7440.13090225788800466No Hit
AAAAAGTACTCTGCGTTGATACCAC7010.12333667040254202No Hit
TATCAACGCAGAGTACTTTTTTTTT6990.12298478261252052No Hit
ATTCCATTCCATTCCATTCCATTCC6970.122632894822499No Hit
GGAAGCAGTGGTATCAACGCAGAGT6210.10926115880168133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG250.0060279418.9997359
ATCGAGA250.006031012618.99805810
GCCACCG350.002177075516.27543615
GTCCTAG508.6928725E-515.2011291
GTGTAGA508.6928725E-515.2011291
CAGGACT400.00526177914.2548324
ATAGGGC400.005271029714.2510593
CAAGACC954.8530637E-914.0047474
AATACAG551.9525376E-413.8216475
GGTGACA551.9598595E-413.81555112
TCGAGAC500.001499648713.29746811
TCTGTCA957.355629E-812.9998198
TTAGGGT604.0732318E-412.6709624
AGAGATG604.0762662E-412.6698455
TCTATAC751.4735371E-512.6676073
GTGACAG604.094514E-412.66313913
TGTACTG853.919831E-612.2972015
CCCACTC550.003062607712.0918063
GTGATCA550.003064632612.090749
CGAGACC658.022433E-411.69008312
Produced by FastQC (version 0.11.5)