FastQCFastQC Report
Wed 25 May 2016
SRR1294863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568363
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC175413.0862318623837233No Hit
CCCATGTACTCTGCGTTGATACCAC119762.1071040866488495No Hit
GAGTACATGGGAAGCAGTGGTATCA83481.4687796355498157No Hit
GTATCAACGCAGAGTACTTTTTTTT47330.8327424550859223No Hit
CATGTACTCTGCGTTGATACCACTG46460.8174353362199861No Hit
GTATCAACGCAGAGTACATGGGAAG41450.729287444819596No Hit
GCGTTGATACCACTGCTTCCCATGT39160.688996292862132No Hit
ACGCAGAGTACATGGGAAGCAGTGG37370.6575023356552062No Hit
GCTTCCCATGTACTCTGCGTTGATA35910.6318145269836355No Hit
GGTATCAACGCAGAGTACTTTTTTT31950.5621407445593749No Hit
TATCAACGCAGAGTACATGGGAAGC29730.5230811998669864No Hit
GGTATCAACGCAGAGTACATGGGAA26120.4595654537681024No Hit
TATCAACGCAGAGTACTTTTTTTTT25340.4458418299572632No Hit
ACTCTGCGTTGATACCACTGCTTCC23620.4155794800154127No Hit
GTGGTATCAACGCAGAGTACATGGG21620.3803907010132609No Hit
CAGTGGTATCAACGCAGAGTACATG20570.36191659203713117No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19930.3506561827564426No Hit
GCAGTGGTATCAACGCAGAGTACAT18750.3298948031451731No Hit
CATGGGAAGCAGTGGTATCAACGCA16700.2938263046679675No Hit
GGGAAGCAGTGGTATCAACGCAGAG16400.28854798781764474No Hit
ACATGGGAAGCAGTGGTATCAACGC15020.26426773030615996No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14080.24772900417514865No Hit
AAGCAGTGGTATCAACGCAGAGTAC12910.22714356845888983No Hit
GCAGAGTACATGGGAAGCAGTGGTA11790.20743785221768482No Hit
ATACCACTGCTTCCCATGTACTCTG11400.20057604031226522No Hit
CTGCTTCCCATGTACTCTGCGTTGA11150.19617744293699627No Hit
GTACTCTGCGTTGATACCACTGCTT10980.19318639672181334No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10950.19265856503678108No Hit
ATGGGAAGCAGTGGTATCAACGCAG10450.18386137028624314No Hit
GTACATGGTAAGCAGTGGTATCAAC10330.18175004354611402No Hit
GATACCACTGCTTCCCATGTACTCT9320.16397971015002735No Hit
CCATGTACTCTGCGTTGATACCACT8770.15430279592443563No Hit
TACCACTGCTTCCCATGTACTCTGC8530.1500801424441774No Hit
CCACTGCTTCCCATGTACTCTGCGT8170.14374616222379008No Hit
GGAAGCAGTGGTATCAACGCAGAGT8000.14075511600860718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7820.13758812589841352No Hit
GTTGATACCACTGCTTCCCATGTAC7720.13582868694830594No Hit
ATTCCATTCCATTCCATTCCATTCC7700.1354767991582844No Hit
CTTCCCATGTACTCTGCGTTGATAC7600.13371736020817682No Hit
AAAAAGTACTCTGCGTTGATACCAC6920.1217531753474452No Hit
GTGGTATCAACGCAGAGTACTTTTT6540.11506730733703636No Hit
ATCAACGCAGAGTACATGGGAAGCA6260.1101408782767351No Hit
TTCCCATGTACTCTGCGTTGATACC6260.1101408782767351No Hit
ACCATGTACTCTGCGTTGATACCAC5960.10486256142641234No Hit
GAATGGAATGGAATGGAATGGAATG5910.10398284195135855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATT400.00531629114.2327137
GTCCAGA551.9015197E-413.8648461
CCAGACC500.001463821713.3401983
CTATACT801.9266026E-613.1019794
TATGACA603.9822003E-412.704954
TCTACAC603.9822003E-412.704953
TAGGACT603.9822003E-412.704954
TCTGGGT701.0582991E-412.2512033
CTCACTC1353.5106495E-1011.999123
GTCTCAC1209.469659E-911.9151021
GTAATAC657.809354E-411.7276463
TAGACAG907.1913273E-611.6462045
CATACTG752.0121754E-411.4344555
TCACTCT1451.1496013E-911.1715944
GTCTCGC600.005722407711.1207621
ATTAGAC600.0057374911.1168323
GTTGTCT600.005921143611.0698888
ACACTGT951.3648927E-510.9934256
TTAGACT700.001451847410.8899574
TTAGACA803.6592095E-410.7198024
Produced by FastQC (version 0.11.5)