FastQCFastQC Report
Wed 25 May 2016
SRR1294862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036877
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC43450.10763270716447391No Hit
CCCATGTACTCTGCGTTGATACCAC43030.10659229894792435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1600.013.0601959
CGGACGT957.417657E-812.99738510
ACCGTCT1900.011.9978838
CGACCGC1603.6379788E-1211.8726110
CGTCGTA658.0401805E-411.6902459
AAGACCG1001.9285017E-611.40166955
CGCCTAA1501.7826096E-1011.393887514
TCGGACG855.33709E-511.1744999
TGTGCGA855.33844E-511.17422110
ACGGGTC2250.010.9793845
TCGTTCA2200.010.79247813
GTATTAA6550.010.5885481
CGGGACT2700.010.5572324
GCGGGAC2700.010.5561833
ATCGACC1909.094947E-1210.4981488
CTTATAC3600.010.2922783
GTACCGT1303.868954E-710.2264256
CCGTCTG2650.010.0359659
GCTCGTT2750.010.01554411
TGCTCGT2850.09.99798810
Produced by FastQC (version 0.11.5)