FastQCFastQC Report
Wed 25 May 2016
SRR1294862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036877
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC44020.10904468974407691No Hit
GTACATGGGAAGCAGTGGTATCAAC42050.10416467977597535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT2650.012.531318
ACGGGTC1404.9112714E-1112.2536375
CCGTCTG2450.011.6179499
GAACCGT907.5308526E-611.602476
TCGTCTA1553.2014214E-1011.01831314
CGTGCGC700.001508123710.84341910
TAAGACG909.217228E-510.5895635
GTACGAC1105.823891E-610.3970253
TCGACCG2053.6379788E-1210.1822359
CCGCCTA2002.5465852E-119.96239213
TTAGGAC2350.09.7333843
CGACCGC2151.0913936E-119.70864310
ATCGACC2550.09.674038
GCTCGTT2750.09.660501511
TACGACC1304.072688E-69.5306074
CGGGACT2106.184564E-119.5306064
ACGACAG1106.605363E-59.5306065
GACGCGT1201.7170372E-59.492936
ACGCGTG1106.929764E-59.4889327
ACCGCGT800.00456546859.4879918
Produced by FastQC (version 0.11.5)