FastQCFastQC Report
Wed 25 May 2016
SRR1294861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386538
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC263446.815371321836404No Hit
CCCATGTACTCTGCGTTGATACCAC253846.567012816333712No Hit
GAGTACATGGGAAGCAGTGGTATCA127283.2928198521232064No Hit
CATGTACTCTGCGTTGATACCACTG106192.7472072603469773No Hit
GCGTTGATACCACTGCTTCCCATGT89212.307923153739089No Hit
ACGCAGAGTACATGGGAAGCAGTGG84732.1920225178378323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77261.998768555743549No Hit
TATCAACGCAGAGTACATGGGAAGC76251.9726391713104534No Hit
GGTATCAACGCAGAGTACATGGGAA76151.9700521035448004No Hit
GTATCAACGCAGAGTACATGGGAAG75541.9542709901743165No Hit
ACTCTGCGTTGATACCACTGCTTCC73331.8970967925533841No Hit
GTGGTATCAACGCAGAGTACATGGG50741.3126781842923594No Hit
GTACTCTGCGTTGATACCACTGCTT48211.2472253698213371No Hit
GCTTCCCATGTACTCTGCGTTGATA47121.2190263311757188No Hit
ATACCACTGCTTCCCATGTACTCTG42841.108299830805768No Hit
GATACCACTGCTTCCCATGTACTCT40671.0521604602910968No Hit
GGGAAGCAGTGGTATCAACGCAGAG38671.0004191049780358No Hit
CAGTGGTATCAACGCAGAGTACATG36540.9453145615696258No Hit
CTGCTTCCCATGTACTCTGCGTTGA36430.9424687870274074No Hit
GCAGAGTACATGGGAAGCAGTGGTA32800.8485582271342015No Hit
CATGGGAAGCAGTGGTATCAACGCA32580.8428666780497649No Hit
TACCACTGCTTCCCATGTACTCTGC31380.8118218648619282No Hit
GTTGATACCACTGCTTCCCATGTAC31310.810010917425971No Hit
ACATGGGAAGCAGTGGTATCAACGC30250.7825879991100487No Hit
GCAGTGGTATCAACGCAGAGTACAT30080.7781899839084384No Hit
CTTCCCATGTACTCTGCGTTGATAC27410.7091152745655019No Hit
CCACTGCTTCCCATGTACTCTGCGT26830.6941102815247142No Hit
CCATGTACTCTGCGTTGATACCACT24610.6366773771272165No Hit
ATGGGAAGCAGTGGTATCAACGCAG24250.6273639331708655No Hit
AAGCAGTGGTATCAACGCAGAGTAC24040.621931090862994No Hit
TGATACCACTGCTTCCCATGTACTC21220.548975779871578No Hit
TTCCCATGTACTCTGCGTTGATACC20950.5419906969043148No Hit
ATCAACGCAGAGTACATGGGAAGCA17780.4599806487331129No Hit
GTACATGGTAAGCAGTGGTATCAAC17230.4457517760220211No Hit
GTATCAACGCAGAGTACTTTTTTTT16100.4165179102701417No Hit
AAACAAAAAAAAAAAAAAAAAAAAA12970.33554268920520103No Hit
ACCATGTACTCTGCGTTGATACCAC12710.32881631301450315No Hit
GGAAGCAGTGGTATCAACGCAGAGT12250.3169158012924991No Hit
GGTATCAACGCAGAGTACTTTTTTT11640.3011346879220154No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10130.26206996466065435No Hit
CTGCGTTGATACCACTGCTTCCCAT9940.25715453590591353No Hit
TATCAACGCAGAGTACTTTTTTTTT9890.255861002023087No Hit
AAAAAGTACTCTGCGTTGATACCAC9850.2548261749168258No Hit
TCAACGCAGAGTACATGGGAAGCAG9070.23464704634473194No Hit
CTCTGCGTTGATACCACTGCTTCCC9040.23387092601503603No Hit
TGGGAAGCAGTGGTATCAACGCAGA8570.22171170751646668No Hit
AAAGTACTCTGCGTTGATACCACTG8150.21084602290072385No Hit
TCCATGTACTCTGCGTTGATACCAC8010.2072241280288096No Hit
GGTAAGCAGTGGTATCAACGCAGAG7890.20411964671002594No Hit
CCCCATGTACTCTGCGTTGATACCA7590.19635844341306677No Hit
TCCCATGTACTCTGCGTTGATACCA7080.18316439780823618No Hit
AGTGGTATCAACGCAGAGTACATGG7060.1826469842551056No Hit
AACGCAGAGTACATGGGAAGCAGTG6840.17695543517066886No Hit
GTACATGGGTAAGCAGTGGTATCAA6680.17281612674562397No Hit
GAGTACATGGTAAGCAGTGGTATCA6560.1697116454268403No Hit
ATGTACTCTGCGTTGATACCACTGC6540.1691942318737097No Hit
GTACATGGAAAGCAGTGGTATCAAC6220.16091561502361995No Hit
TTGATACCACTGCTTCCCATGTACT6170.1596220811407934No Hit
ACTGCTTCCCATGTACTCTGCGTTG5960.154189238832922No Hit
GCTTACCATGTACTCTGCGTTGATA5690.14720415586565874No Hit
ACTCTGCGTTGATACCACTGCTTAC5340.13814941868587305No Hit
CAACGCAGAGTACATGGGAAGCAGT5300.13711459157961184No Hit
AAAAACAAAAAAAAAAAAAAAAAAA5100.13194045604830573No Hit
GAACAAAAAAAAAAAAAAAAAAAAA5050.13064692216547918No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5020.12987080183578328No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4920.12728373407013024No Hit
GCGTTGATACCACTGCTTACCATGT4530.11719416978408333No Hit
ACGCAGAGTACATGGTAAGCAGTGG4490.1161593426778221No Hit
CGTTGATACCACTGCTTCCCATGTA4380.11331356813560375No Hit
CGCAGAGTACATGGGAAGCAGTGGT4380.11331356813560375No Hit
GAGTACATGGAAAGCAGTGGTATCA4320.1117613274762119No Hit
CTGCTTACCATGTACTCTGCGTTGA4020.10400012417925275No Hit
GCTTTCCATGTACTCTGCGTTGATA3890.1006369360839038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA456.744973E-414.7780821
GAACAAA3050.013.7051981
CGCAAAA905.3683834E-712.6669272
GAGAACA907.4181244E-611.611351
AAAAGTA1855.456968E-1210.7840042
AAAAAGT1855.456968E-1210.7840041
GAGAAAA1158.747247E-710.7393511
TACAAAA909.469669E-510.5557732
GAAACAA1002.3850198E-510.4502131
ACGCAAA1106.0045422E-610.3638491
AGAACAA750.002639407310.133542
AATCAAC2750.09.01474519
GAAAACA1856.4877895E-88.7299081
ATGGGTA1909.978976E-88.5001745
GCAAAAA1803.9867155E-78.4446183
GGAAAAA2159.558789E-98.395521
GGGTAAG1701.5979786E-68.3814377
GAAAAAA9350.08.3317211
GAATCAA1150.00110816788.25889618
AGTACTC2605.14774E-108.0386265
Produced by FastQC (version 0.11.5)