##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294861_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386538
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74778417645872	33.0	31.0	34.0	25.0	34.0
2	30.914789749002686	33.0	31.0	34.0	26.0	34.0
3	31.24233063760872	34.0	31.0	34.0	26.0	34.0
4	34.058617781434165	37.0	35.0	37.0	27.0	37.0
5	34.82734685852361	37.0	35.0	37.0	31.0	37.0
6	34.58494378301745	37.0	36.0	37.0	30.0	37.0
7	34.81008852945894	37.0	36.0	37.0	31.0	37.0
8	34.677982501073636	37.0	37.0	37.0	31.0	37.0
9	36.18023842416528	39.0	38.0	39.0	28.0	39.0
10	36.40260465982646	39.0	38.0	39.0	31.0	39.0
11	36.37592164289151	39.0	39.0	39.0	30.0	39.0
12	36.471143846141906	39.0	38.0	39.0	31.0	39.0
13	36.14911082480894	39.0	38.0	39.0	30.0	39.0
14	37.68915604675349	41.0	39.0	41.0	30.0	41.0
15	37.562935597535045	41.0	39.0	41.0	30.0	41.0
16	37.51395981766346	41.0	38.0	41.0	30.0	41.0
17	37.626740449839346	41.0	39.0	41.0	30.0	41.0
18	37.59020587885279	41.0	39.0	41.0	30.0	41.0
19	37.54424920706373	41.0	39.0	41.0	30.0	41.0
20	37.60037046810404	41.0	39.0	41.0	30.0	41.0
21	37.55860225902757	41.0	39.0	41.0	30.0	41.0
22	37.63627379455578	41.0	39.0	41.0	30.0	41.0
23	37.652484361175354	41.0	39.0	41.0	29.0	41.0
24	37.78947218643445	41.0	39.0	41.0	30.0	41.0
25	37.02101733852817	41.0	38.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32452180126647434
1101	2	0.16112275534724674
1101	3	0.13198854597220233
1101	4	0.4050005671032295
1101	5	0.3154969471761788
1101	6	0.17157081220955916
1101	7	0.4863686349250713
1101	8	0.35713923472666664
1101	9	0.5618267224936986
1101	10	0.3513885449723517
1101	11	0.428461935084151
1101	12	0.48845700056324404
1101	13	0.3271300156408685
1101	14	0.5387765384388459
1101	15	0.382951176036471
1101	16	0.5500474917071188
1101	17	0.3821711585830698
1101	18	0.5188131482472684
1101	19	0.3119782999078211
1101	20	0.13522434561767938
1101	21	0.23127177207271643
1101	22	0.43976351789588364
1101	23	0.3154775330168178
1101	24	0.3000337828901749
1101	25	0.3037089054601907
1102	1	-0.17345830399703033
1102	2	-0.24462584871740134
1102	3	-0.07088739160241886
1102	4	-0.1521698138799934
1102	5	0.04369271504693728
1102	6	0.05442345966187645
1102	7	-0.18326490546791518
1102	8	-0.05703241216629351
1102	9	-0.08416996062652515
1102	10	-0.06166439098962684
1102	11	-0.1968647644896251
1102	12	-0.2551815576735095
1102	13	-0.2661531392548042
1102	14	-0.17514094518119805
1102	15	-0.10274600657837141
1102	16	0.22291940584769065
1102	17	0.07423452603381264
1102	18	0.09524694640172271
1102	19	-0.05072283090612473
1102	20	-0.07415450812885638
1102	21	0.029334635776436357
1102	22	0.057563451819973466
1102	23	-0.09858289699995737
1102	24	0.018749947608910134
1102	25	-0.276974031581247
1103	1	0.06787196377213434
1103	2	0.003123608377986642
1103	3	0.0169914059396028
1103	4	-0.009327704253124125
1103	5	-0.049706011319578636
1103	6	-0.1057501422590974
1103	7	0.20234246209046347
1103	8	-0.10215169173746119
1103	9	0.11786389876357362
1103	10	-0.09597978907184057
1103	11	-0.09979884104156866
1103	12	-0.03356805473497104
1103	13	0.12243900784125827
1103	14	-0.0688623848908847
1103	15	-0.016713563045229307
1103	16	0.051804801155590496
1103	17	-0.05814809974307167
1103	18	-0.16292372813932587
1103	19	-0.057932859255153346
1103	20	-0.14020869973511907
1103	21	-0.11699290551141672
1103	22	-0.114680011799841
1103	23	-0.42308838344568045
1103	24	-0.28875249984653095
1103	25	-0.0084044616637442
1104	1	-0.5267063971952304
1104	2	-0.37209750789617146
1104	3	-0.5414690967533957
1104	4	-0.22640448110389855
1104	5	-1.0137333637327117
1104	6	-0.7673036325959828
1104	7	-0.8597714094904845
1104	8	-0.7845132135454662
1104	9	-1.0020240249876124
1104	10	-1.3411251317690684
1104	11	-0.9461758343882778
1104	12	-0.836880178507279
1104	13	-0.8061437358486785
1104	14	-0.9855741548771562
1104	15	-0.8192039263359661
1104	16	-0.7718434193534378
1104	17	-1.1215391653255011
1104	18	-1.1119283748813231
1104	19	-0.9340879442694074
1104	20	-1.0518050932296816
1104	21	-1.0477439087852574
1104	22	-1.0180654009644812
1104	23	-1.1366471013673234
1104	24	-1.0677385332185878
1104	25	-0.860530478138557
1105	1	0.3893697482888676
1105	2	0.41572442139367993
1105	3	0.09745577371292669
1105	4	0.509600801838296
1105	5	0.3489228386446115
1105	6	0.21316472054837732
1105	7	0.1516421585273946
1105	8	0.15140253876582221
1105	9	0.4038767420549547
1105	10	0.1731711955701627
1105	11	0.0331146512360192
1105	12	0.39967752747666907
1105	13	0.4738775991101747
1105	14	0.32826880458642194
1105	15	0.46411607264808197
1105	16	0.289405691689943
1105	17	0.39138880378960295
1105	18	0.3816076405611213
1105	19	0.279229895819995
1105	20	0.3203978682658004
1105	21	0.20667230070371545
1105	22	0.42573666649462893
1105	23	0.21114447640368184
1105	24	0.3683359613460766
1105	25	0.16301099803740726
1106	1	-0.3696756915119437
1106	2	-0.4183146622706104
1106	3	-0.1172939698498432
1106	4	-0.219785589892588
1106	5	-0.33401553751593127
1106	6	-0.611660156931606
1106	7	-0.41449234608847263
1106	8	-0.2923171599971397
1106	9	-0.32695621867727453
1106	10	-0.15270134855019535
1106	11	-0.09360479328147875
1106	12	-0.32745572344606444
1106	13	-0.28377387419843103
1106	14	-0.3086774444163183
1106	15	-0.41683568208395627
1106	16	-0.17789746533731687
1106	17	-0.36044132517421446
1106	18	-0.3896854167843813
1106	19	-0.41100269494125286
1106	20	-0.03011018430164114
1106	21	-0.4111266349721774
1106	22	-0.41950675161641726
1106	23	-0.37298008633796087
1106	24	-0.32309772004052917
1106	25	-0.10342947783995982
1107	1	-0.22422524453180515
1107	2	-0.38118948785847806
1107	3	-0.11935695510078048
1107	4	-0.34747381946318967
1107	5	-0.2579675666887695
1107	6	-0.19501417718481662
1107	7	-0.1257885456872927
1107	8	-0.36789717842989944
1107	9	-0.2067067201567454
1107	10	-0.5396266445158133
1107	11	-0.3964070862482103
1107	12	-0.2420173176799807
1107	13	-0.3171297491410101
1107	14	-0.345437387691355
1107	15	-0.4376538568984145
1107	16	-0.30565702911437853
1107	17	-0.2416413377290354
1107	18	-0.12734103235577976
1107	19	0.023610944729696826
1107	20	-0.18720522714496468
1107	21	-0.17889099240791495
1107	22	-0.24142803859963635
1107	23	0.08817558487412214
1107	24	0.13071338127402043
1107	25	-0.013847981362438588
1108	1	0.6173089904167668
1108	2	0.4220810078810011
1108	3	0.47136202907442026
1108	4	0.5812503689155832
1108	5	0.5006316537974769
1108	6	0.433806612826686
1108	7	0.3153235457101431
1108	8	0.271717308464666
1108	9	0.41311280789123117
1108	10	0.48321992082150445
1108	11	0.4086279248637652
1108	12	0.37754814248999224
1108	13	0.6407787417516317
1108	14	0.3814033480445431
1108	15	0.4089198562896641
1108	16	0.5937097555589119
1108	17	0.34554303179175605
1108	18	0.4351596656328951
1108	19	0.5777692820648355
1108	20	0.427633453712275
1108	21	0.3738937032131844
1108	22	0.45367933997825105
1108	23	0.5136499379782222
1108	24	0.49182759455702296
1108	25	0.591590188008702
1109	1	0.083399960009924
1109	2	0.03163892489770248
1109	3	-0.4414896898341212
1109	4	-0.3429370908529066
1109	5	-0.02631937012868235
1109	6	-0.18897724438007657
1109	7	-0.20261289122321102
1109	8	-0.21579592020308525
1109	9	-0.494231966648627
1109	10	-0.2656726896719661
1109	11	-0.5342869643427335
1109	12	-0.28739016009463825
1109	13	-0.11942506155853039
1109	14	-0.4658259961408433
1109	15	-0.2879418127590725
1109	16	-0.19946479739395073
1109	17	-0.005273054268229771
1109	18	-0.004904922996452399
1109	19	0.06643899632560846
1109	20	-0.11620389640827966
1109	21	0.06782869999466357
1109	22	0.22102935143400515
1109	23	0.18504710349749587
1109	24	0.27802232054630593
1109	25	0.19636396415236135
1110	1	0.6207478092064314
1110	2	0.6366510758069737
1110	3	0.7164562644023036
1110	4	0.8371872477224613
1110	5	0.1708691289497537
1110	6	0.6662484399184123
1110	7	0.5120271694916738
1110	8	0.5004545657010695
1110	9	0.4594438486787098
1110	10	0.4505936624902134
1110	11	0.4232681510710705
1110	12	0.5740524529722819
1110	13	0.42974050548830434
1110	14	0.5231390214045959
1110	15	0.6031918578203914
1110	16	0.4895459678621208
1110	17	0.34609845151899066
1110	18	0.5061789804203514
1110	19	0.5594856087473019
1110	20	0.3029271086398353
1110	21	0.42105920234683225
1110	22	0.2925491538462097
1110	23	0.38054320172020795
1110	24	0.28486332213029186
1110	25	0.5610155279043099
1111	1	0.11828660000364621
1111	2	0.3083471699749758
1111	3	0.32499915721485806
1111	4	0.37303529480646347
1111	5	0.2697633682934324
1111	6	0.4826029967224059
1111	7	0.14174002841801325
1111	8	0.11884229532475388
1111	9	0.23336929900865044
1111	10	0.6271230008173063
1111	11	0.2905947071616737
1111	12	0.4113807925478099
1111	13	-0.0817273026087122
1111	14	0.2507295332647672
1111	15	0.11375008860333935
1111	16	-0.012576379204048749
1111	17	0.3542311427977438
1111	18	0.3779642488348287
1111	19	0.277338292892118
1111	20	0.2737492951923528
1111	21	0.35626519485619923
1111	22	0.16813324516063233
1111	23	0.5231684915365094
1111	24	0.2937985816273496
1111	25	0.476531846791417
1112	1	-0.17547114811857156
1112	2	0.14136693224435604
1112	3	0.26720241932623523
1112	4	0.16261452619274053
1112	5	0.16520321855717413
1112	6	0.2554195454960748
1112	7	0.11727176229584302
1112	8	0.1481308050114265
1112	9	0.401292680714576
1112	10	0.15687335935785995
1112	11	0.20131932370558303
1112	12	-0.17643855162681632
1112	13	0.08960889904908242
1112	14	0.12967165631102517
1112	15	0.3983243368835616
1112	16	0.2729385822680328
1112	17	0.17386214987132576
1112	18	0.33110603643297054
1112	19	0.24367194222730149
1112	20	0.3146399342453208
1112	21	0.10830340677189554
1112	22	0.2384120560213745
1112	23	0.2769811753645044
1112	24	0.3008065302892007
1112	25	0.12900030737626622
1113	1	0.00866147335724321
1113	2	0.18656398861732626
1113	3	0.07954154154447579
1113	4	0.11822273148326445
1113	5	0.06983091304182665
1113	6	0.11080292917765888
1113	7	-0.04177905297341056
1113	8	-0.011823020446946941
1113	9	-0.17449966417945717
1113	10	-0.07210023457614767
1113	11	0.22429953661118418
1113	12	-0.03448209161294358
1113	13	0.09672592265302171
1113	14	-0.13049302668119367
1113	15	0.22060588056551467
1113	16	0.07816999026572802
1113	17	0.07652090976836234
1113	18	0.1674610404592798
1113	19	0.2072606164314692
1113	20	0.2757080928958331
1113	21	0.3920301391317764
1113	22	0.16958545725114504
1113	23	0.34143934597757664
1113	24	0.20453819662228057
1113	25	0.5699462338228258
1114	1	0.2683938501108969
1114	2	0.5502605118883181
1114	3	0.32208195429765496
1114	4	0.3335672553384299
1114	5	0.4874810860111509
1114	6	0.42086983498924724
1114	7	0.4729712596492419
1114	8	0.12553470201715555
1114	9	0.3855786512180046
1114	10	0.6449694186637203
1114	11	0.262151978718947
1114	12	0.3518784330454494
1114	13	0.41930229842088806
1114	14	0.4781855607207959
1114	15	0.4330980079512585
1114	16	0.34229277566510774
1114	17	0.45985104841765434
1114	18	0.35488402575460753
1114	19	0.3768663924202187
1114	20	0.41223063367369406
1114	21	0.5248194634104664
1114	22	0.556507333534725
1114	23	0.7350518034198217
1114	24	0.5487106365394041
1114	25	0.2637905340501021
1115	1	0.5992821402136883
1115	2	0.5802361068185959
1115	3	0.2967106615547621
1115	4	0.3677842517910719
1115	5	0.4438150634931617
1115	6	0.5673773697143005
1115	7	0.38104852120082455
1115	8	0.3359717251430041
1115	9	0.3647844431126188
1115	10	0.41699975715931714
1115	11	0.4910742544690194
1115	12	0.1893216164100835
1115	13	0.21140557995014575
1115	14	0.25725617211769247
1115	15	0.45287155793629097
1115	16	0.27522873277025184
1115	17	0.4151144689378725
1115	18	0.4437629348752097
1115	19	0.5682556668910692
1115	20	0.47127003561340075
1115	21	0.5358652524018765
1115	22	0.46995552492853676
1115	23	0.45031086913206053
1115	24	0.40107810975279534
1115	25	0.2898105117317158
1116	1	-0.01202306987657309
1116	2	-0.1714366453916618
1116	3	-0.07866234828713914
1116	4	-0.17597142444564895
1116	5	-0.19070016272224422
1116	6	-0.16661795955376135
1116	7	-0.1313001897142314
1116	8	-0.48553495659851365
1116	9	-0.13770383372092
1116	10	-0.2732908661609841
1116	11	-0.2763251487152374
1116	12	-0.11920154263575
1116	13	-0.10017116277036564
1116	14	-0.04082271135495574
1116	15	-0.0909582569025531
1116	16	-0.13682898413149758
1116	17	-0.13931253234102314
1116	18	-0.3714465993490208
1116	19	-0.23977632391574843
1116	20	-0.29590695176450765
1116	21	-0.49497640607865634
1116	22	-0.26794134827591876
1116	23	-0.21701068851997007
1116	24	-0.192952806044282
1116	25	-0.40447896244025117
1201	1	0.06259756021954388
1201	2	0.11306554727104867
1201	3	0.008690866683533471
1201	4	0.09615708465586437
1201	5	0.13194090231740319
1201	6	-0.022196631843215187
1201	7	0.08746418035788395
1201	8	0.2744413783461397
1201	9	0.25846433397571644
1201	10	0.4356485804580572
1201	11	0.2544001684077202
1201	12	0.5141723540231027
1201	13	0.3234669749804411
1201	14	0.3724381313901901
1201	15	0.3886376426499467
1201	16	0.5236587851846792
1201	17	0.19739297791570465
1201	18	0.16142090998801706
1201	19	0.3038610287384387
1201	20	0.26872332380440866
1201	21	0.11767374365048511
1201	22	0.32050259021736593
1201	23	0.07448447192012964
1201	24	0.1660802913862085
1201	25	-0.17231467536406342
1202	1	0.06657515971575734
1202	2	-0.044268111638547936
1202	3	-0.13230828831754238
1202	4	-0.3570257981720246
1202	5	-0.2901858603728442
1202	6	-0.06997601965274214
1202	7	-0.13894733733086184
1202	8	-0.42059754622405165
1202	9	0.004524486332556421
1202	10	-0.38308147933445014
1202	11	0.08245734559724838
1202	12	-0.3807329129226602
1202	13	0.040620453130955525
1202	14	-0.04191978188015355
1202	15	-0.34865122810377613
1202	16	-0.36528650836408616
1202	17	-0.46049812324962147
1202	18	-0.07206225268551947
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2210	4	0.37248158495042816
2210	5	0.39028754695714696
2210	6	0.3640721735404213
2210	7	0.2177459346564774
2210	8	0.26166252419149316
2210	9	0.28881411259300194
2210	10	0.5043470687390439
2210	11	0.32318976068695804
2210	12	0.4186528486570751
2210	13	0.48401651429789183
2210	14	0.5162518662289628
2210	15	0.5334990734686045
2210	16	0.3134848980200218
2210	17	0.21923667989630502
2210	18	0.32233486529847255
2210	19	0.43034678310991126
2210	20	0.4825717388985211
2210	21	0.4417579966280698
2210	22	0.40076521267632614
2210	23	0.379609982861723
2210	24	0.16046524829401676
2210	25	0.30632143704612247
2211	1	0.26765397980581085
2211	2	0.20846219182912762
2211	3	0.29921125751391386
2211	4	0.12838381102454832
2211	5	0.1304719029150121
2211	6	0.09053950465891347
2211	7	0.2570379350202643
2211	8	0.018014137974851963
2211	9	0.06695568042111688
2211	10	-0.008901843903196038
2211	11	0.07157009248488322
2211	12	0.14754366349328762
2211	13	0.12572611354035246
2211	14	0.20343254683734813
2211	15	0.004778383979463285
2211	16	0.14187496655164722
2211	17	0.15441517776439184
2211	18	0.15387867257533827
2211	19	0.23938108102186817
2211	20	0.030501078031093698
2211	21	0.13434202945477125
2211	22	0.30192666591057815
2211	23	0.061769825790015886
2211	24	0.16748158139730407
2211	25	0.31819276236537775
2212	1	-0.09103600227670228
2212	2	-0.04841562904134378
2212	3	-0.05526017656560356
2212	4	-0.10125489075132776
2212	5	0.02171954982707547
2212	6	0.12027346397034222
2212	7	-0.1017654488902835
2212	8	-0.16971124815837157
2212	9	-0.14637583848296742
2212	10	-0.05836772129313772
2212	11	0.06839484129983475
2212	12	-4.4572209560556075E-4
2212	13	-0.20665464443746373
2212	14	0.050997809589382825
2212	15	-0.3202539355415297
2212	16	-1.1235533521528538
2212	17	-0.5235689913404116
2212	18	-0.4011325542056312
2212	19	-0.5496234777316218
2212	20	-0.4194320148903614
2212	21	-0.45936190105258845
2212	22	-0.41572546264173127
2212	23	-0.2162234154610303
2212	24	-0.06544577339165158
2212	25	-0.11450325269438366
2213	1	-0.3512517496873748
2213	2	-0.47100579258409425
2213	3	-0.19232787537934826
2213	4	-0.47018535223097757
2213	5	-0.10640287428502404
2213	6	-0.4188752536108069
2213	7	-0.29778499278639714
2213	8	-0.34148728179872023
2213	9	-0.565346319554294
2213	10	-0.27070809090692194
2213	11	-0.15463732967341315
2213	12	-0.3835180321220051
2213	13	-0.5585236190996881
2213	14	-0.3400616941676802
2213	15	-0.26270695731935234
2213	16	-0.4166233924113669
2213	17	-0.47593970416698994
2213	18	-0.3450334489720248
2213	19	-0.3801196012069923
2213	20	-0.2912331354389295
2213	21	-0.47081586657601093
2213	22	-0.29397750244629606
2213	23	-0.23116326661204312
2213	24	-0.25665579859802534
2213	25	-0.3371722608058221
2214	1	0.2502842771232743
2214	2	0.4780190631224812
2214	3	0.4996894398319718
2214	4	0.32004458632795263
2214	5	0.2264029346890979
2214	6	0.1860679599434718
2214	7	0.36301374115514307
2214	8	0.3911951795678803
2214	9	0.2492761902204208
2214	10	0.40596059276465013
2214	11	0.3328324152530442
2214	12	0.40747061058885237
2214	13	0.10517261996194094
2214	14	0.3712676672175377
2214	15	0.42124621713605137
2214	16	0.209684557691034
2214	17	0.4018647084191187
2214	18	0.4274367614170984
2214	19	0.5312097601782213
2214	20	0.4857218077746239
2214	21	0.31107065362507313
2214	22	0.29394141395660967
2214	23	0.4740056963371302
2214	24	0.42244260021038826
2214	25	0.4422403253047733
2215	1	0.2518225675821668
2215	2	0.10145760563860406
2215	3	0.2436752801462987
2215	4	0.14405859774466023
2215	5	0.2522017337105211
2215	6	0.29469350668525607
2215	7	0.11931517195060337
2215	8	0.1097575266655113
2215	9	0.17656416560777188
2215	10	0.33215508457278986
2215	11	0.26381321442273276
2215	12	0.2907458719128897
2215	13	0.03137637858007025
2215	14	0.3375920797017855
2215	15	0.354344482389223
2215	16	0.18759248266694328
2215	17	0.09171847815742495
2215	18	0.17202031097600212
2215	19	0.029449066279489955
2215	20	0.2741883360569304
2215	21	0.012090929405339068
2215	22	0.3367587095307769
2215	23	0.3900715648225628
2215	24	0.529766160148121
2215	25	0.38909516999304117
2216	1	0.19472770276053808
2216	2	0.3253897764912672
2216	3	0.06276022655487523
2216	4	0.04095509430521105
2216	5	-0.03692000681099472
2216	6	0.15770742445841535
2216	7	0.2899438713586946
2216	8	0.22151564536652302
2216	9	0.03303513025343108
2216	10	0.18184313448480793
2216	11	0.10822210899960538
2216	12	0.21029474409333915
2216	13	0.04275055871124778
2216	14	0.012300126414309887
2216	15	0.09027473354903748
2216	16	0.34063999506496145
2216	17	0.2599955538253198
2216	18	0.276627573813947
2216	19	0.46246778328477234
2216	20	0.36414194347974416
2216	21	0.23771882894141072
2216	22	0.39586925184401167
2216	23	0.20779672879632471
2216	24	0.13114194002334045
2216	25	0.20861204729266802
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	329.0
3	49.0
4	21.0
5	33.0
6	25.0
7	115.0
8	99.0
9	215.0
10	241.0
11	295.0
12	447.0
13	751.0
14	1499.0
15	2819.0
16	3886.0
17	4179.0
18	4342.0
19	3970.0
20	3680.0
21	3571.0
22	2992.0
23	2559.0
24	2565.0
25	2100.0
26	1890.0
27	1748.0
28	1574.0
29	1591.0
30	1801.0
31	2172.0
32	2756.0
33	3628.0
34	5142.0
35	8089.0
36	15452.0
37	39065.0
38	260848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.576632987049955	22.169553798983145	10.364438464623465	22.889374749343443
2	10.902823672718318	29.3330223276186	30.806411901152014	28.95774209851106
3	22.39040788767732	35.96420064434693	24.08577123351226	17.559620234463488
4	13.884418320776867	26.740994720842178	24.243955662330862	35.13063129605009
5	9.943466799381012	44.15188534724761	32.29028152548919	13.614366327882196
6	30.76141763702978	32.29456544833626	17.14031787489182	19.803699039742135
7	18.57861759081227	39.43680585427539	21.471290936134707	20.51328561877763
8	27.42446497915873	36.35628851878045	20.31615515539632	15.90309134666449
9	26.164262523611733	22.425770409736458	17.782194226429077	33.62777284022273
10	18.085790106563834	36.56160592113447	27.056122237425882	18.29648173487581
11	23.597802180721676	32.508762522052024	14.997707366735835	28.895727930490466
12	24.153011127375414	38.21495653976191	24.0312447374898	13.600787595372877
13	29.567060970173237	24.916283939993985	19.494956301771772	26.021698788061006
14	20.78866977076807	35.00612787965	21.790636392609233	22.414565956972698
15	36.75566011825489	22.999331512698667	19.102154187373362	21.142854181673084
16	15.459395314973584	31.270128746246307	33.03770148178664	20.232774456993468
17	26.731752311809988	34.40030262752201	23.490424999935225	15.377520060732783
18	26.46027719550082	25.770527314043484	27.82759880498631	19.94159668546939
19	22.580461318467247	36.329606449339934	28.112500777153755	12.977431455039065
20	24.699477340630352	28.109330907903633	24.583129270738592	22.608062480727423
21	18.005540166204987	33.236592046311436	29.054518034044296	19.70334975343928
22	22.65105061228508	31.82146715266066	17.171183353928495	28.356298881125763
23	16.02015831271295	32.02502947311145	26.537200896500796	25.417611317674798
24	21.52802431736966	40.59736147210266	19.932002580999594	17.942611629528084
25	22.159935636230138	26.691697021613415	18.004931431234656	33.143435910921795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8366.0
1	8366.0
2	7210.0
3	6054.0
4	6054.0
5	6054.0
6	5584.0
7	5114.0
8	5114.0
9	5114.0
10	4844.0
11	4574.0
12	4574.0
13	4574.0
14	4263.0
15	3952.0
16	3952.0
17	3952.0
18	4308.5
19	4665.0
20	4665.0
21	4665.0
22	5326.0
23	5987.0
24	5987.0
25	5987.0
26	7020.5
27	8054.0
28	8054.0
29	8054.0
30	8921.0
31	9788.0
32	9788.0
33	9788.0
34	10418.5
35	11049.0
36	11049.0
37	11049.0
38	11682.5
39	12316.0
40	12316.0
41	12316.0
42	17781.0
43	23246.0
44	23246.0
45	23246.0
46	82632.5
47	142019.0
48	142019.0
49	142019.0
50	117171.0
51	92323.0
52	92323.0
53	92323.0
54	60761.5
55	29200.0
56	29200.0
57	29200.0
58	18554.5
59	7909.0
60	7909.0
61	7909.0
62	6756.0
63	5603.0
64	5603.0
65	5603.0
66	4387.0
67	3171.0
68	3171.0
69	3171.0
70	2451.5
71	1732.0
72	1732.0
73	1732.0
74	1301.0
75	870.0
76	870.0
77	870.0
78	614.5
79	359.0
80	359.0
81	359.0
82	245.5
83	132.0
84	132.0
85	132.0
86	79.5
87	27.0
88	27.0
89	27.0
90	23.0
91	19.0
92	19.0
93	19.0
94	11.5
95	4.0
96	4.0
97	4.0
98	4.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013711459157961183
2	0.17928379615975662
3	0.18652758590358515
4	0.17617931484097293
5	0.1932539620942831
6	0.1552240659391832
7	0.163502682789273
8	0.13582105769678532
9	0.15703501337514034
10	0.17229871319249337
11	0.1342688170373935
12	0.14306484744061385
13	0.1836818113613668
14	0.1544479456094873
15	0.15315441172666075
16	0.15134346429070364
17	0.15134346429070364
18	0.1554827727157485
19	0.13297528315456697
20	0.16220914890644644
21	0.16272656245957706
22	0.13582105769678532
23	0.1534131185032261
24	0.16583104377836075
25	0.6392644448928695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	386538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.03276262618423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26025949627194	17.46322483171124
2	12.008454177185463	5.291588407866755
3	3.859566723419245	2.551107523710476
4	1.5992485175835143	1.4094345187277835
5	0.7761404332765808	0.8550258965483343
6	0.4673281277520108	0.6177917824379492
7	0.3170316444548817	0.48895580770842706
8	0.2172253860153819	0.38288602931665194
9	0.1455997181940938	0.2887167626468808
>10	1.0509011918041449	4.509776529086404
>50	0.10685140609405272	1.6112258044487218
>100	0.11507074502436446	5.245797308414697
>500	0.02935478189397053	4.620761736232919
>1k	0.032877355721247	19.635844341306676
>5k	0.00939353020607057	15.605451469195783
>10k+	0.004696765103035285	19.422411250640298
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	26344	6.815371321836404	No Hit
CCCATGTACTCTGCGTTGATACCAC	25384	6.567012816333712	No Hit
GAGTACATGGGAAGCAGTGGTATCA	12728	3.2928198521232064	No Hit
CATGTACTCTGCGTTGATACCACTG	10619	2.7472072603469773	No Hit
GCGTTGATACCACTGCTTCCCATGT	8921	2.307923153739089	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	8473	2.1920225178378323	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7726	1.998768555743549	No Hit
TATCAACGCAGAGTACATGGGAAGC	7625	1.9726391713104534	No Hit
GGTATCAACGCAGAGTACATGGGAA	7615	1.9700521035448004	No Hit
GTATCAACGCAGAGTACATGGGAAG	7554	1.9542709901743165	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7333	1.8970967925533841	No Hit
GTGGTATCAACGCAGAGTACATGGG	5074	1.3126781842923594	No Hit
GTACTCTGCGTTGATACCACTGCTT	4821	1.2472253698213371	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4712	1.2190263311757188	No Hit
ATACCACTGCTTCCCATGTACTCTG	4284	1.108299830805768	No Hit
GATACCACTGCTTCCCATGTACTCT	4067	1.0521604602910968	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	3867	1.0004191049780358	No Hit
CAGTGGTATCAACGCAGAGTACATG	3654	0.9453145615696258	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	3643	0.9424687870274074	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	3280	0.8485582271342015	No Hit
CATGGGAAGCAGTGGTATCAACGCA	3258	0.8428666780497649	No Hit
TACCACTGCTTCCCATGTACTCTGC	3138	0.8118218648619282	No Hit
GTTGATACCACTGCTTCCCATGTAC	3131	0.810010917425971	No Hit
ACATGGGAAGCAGTGGTATCAACGC	3025	0.7825879991100487	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3008	0.7781899839084384	No Hit
CTTCCCATGTACTCTGCGTTGATAC	2741	0.7091152745655019	No Hit
CCACTGCTTCCCATGTACTCTGCGT	2683	0.6941102815247142	No Hit
CCATGTACTCTGCGTTGATACCACT	2461	0.6366773771272165	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	2425	0.6273639331708655	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2404	0.621931090862994	No Hit
TGATACCACTGCTTCCCATGTACTC	2122	0.548975779871578	No Hit
TTCCCATGTACTCTGCGTTGATACC	2095	0.5419906969043148	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1778	0.4599806487331129	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1723	0.4457517760220211	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1610	0.4165179102701417	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1297	0.33554268920520103	No Hit
ACCATGTACTCTGCGTTGATACCAC	1271	0.32881631301450315	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	1225	0.3169158012924991	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1164	0.3011346879220154	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1013	0.26206996466065435	No Hit
CTGCGTTGATACCACTGCTTCCCAT	994	0.25715453590591353	No Hit
TATCAACGCAGAGTACTTTTTTTTT	989	0.255861002023087	No Hit
AAAAAGTACTCTGCGTTGATACCAC	985	0.2548261749168258	No Hit
TCAACGCAGAGTACATGGGAAGCAG	907	0.23464704634473194	No Hit
CTCTGCGTTGATACCACTGCTTCCC	904	0.23387092601503603	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	857	0.22171170751646668	No Hit
AAAGTACTCTGCGTTGATACCACTG	815	0.21084602290072385	No Hit
TCCATGTACTCTGCGTTGATACCAC	801	0.2072241280288096	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	789	0.20411964671002594	No Hit
CCCCATGTACTCTGCGTTGATACCA	759	0.19635844341306677	No Hit
TCCCATGTACTCTGCGTTGATACCA	708	0.18316439780823618	No Hit
AGTGGTATCAACGCAGAGTACATGG	706	0.1826469842551056	No Hit
AACGCAGAGTACATGGGAAGCAGTG	684	0.17695543517066886	No Hit
GTACATGGGTAAGCAGTGGTATCAA	668	0.17281612674562397	No Hit
GAGTACATGGTAAGCAGTGGTATCA	656	0.1697116454268403	No Hit
ATGTACTCTGCGTTGATACCACTGC	654	0.1691942318737097	No Hit
GTACATGGAAAGCAGTGGTATCAAC	622	0.16091561502361995	No Hit
TTGATACCACTGCTTCCCATGTACT	617	0.1596220811407934	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	596	0.154189238832922	No Hit
GCTTACCATGTACTCTGCGTTGATA	569	0.14720415586565874	No Hit
ACTCTGCGTTGATACCACTGCTTAC	534	0.13814941868587305	No Hit
CAACGCAGAGTACATGGGAAGCAGT	530	0.13711459157961184	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	510	0.13194045604830573	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	505	0.13064692216547918	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	502	0.12987080183578328	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	492	0.12728373407013024	No Hit
GCGTTGATACCACTGCTTACCATGT	453	0.11719416978408333	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	449	0.1161593426778221	No Hit
CGTTGATACCACTGCTTCCCATGTA	438	0.11331356813560375	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	438	0.11331356813560375	No Hit
GAGTACATGGAAAGCAGTGGTATCA	432	0.1117613274762119	No Hit
CTGCTTACCATGTACTCTGCGTTGA	402	0.10400012417925275	No Hit
GCTTTCCATGTACTCTGCGTTGATA	389	0.1006369360839038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.587067765653054E-4	0.0
12	0.0	0.0	0.0	2.587067765653054E-4	0.0
13	0.0	0.0	0.0	2.587067765653054E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	45	6.744973E-4	14.778082	1
GAACAAA	305	0.0	13.705198	1
CGCAAAA	90	5.3683834E-7	12.666927	2
GAGAACA	90	7.4181244E-6	11.61135	1
AAAAGTA	185	5.456968E-12	10.784004	2
AAAAAGT	185	5.456968E-12	10.784004	1
GAGAAAA	115	8.747247E-7	10.739351	1
TACAAAA	90	9.469669E-5	10.555773	2
GAAACAA	100	2.3850198E-5	10.450213	1
ACGCAAA	110	6.0045422E-6	10.363849	1
AGAACAA	75	0.0026394073	10.13354	2
AATCAAC	275	0.0	9.014745	19
GAAAACA	185	6.4877895E-8	8.729908	1
ATGGGTA	190	9.978976E-8	8.500174	5
GCAAAAA	180	3.9867155E-7	8.444618	3
GGAAAAA	215	9.558789E-9	8.39552	1
GGGTAAG	170	1.5979786E-6	8.381437	7
GAAAAAA	935	0.0	8.331721	1
GAATCAA	115	0.0011081678	8.258896	18
AGTACTC	260	5.14774E-10	8.038626	5
>>END_MODULE