FastQCFastQC Report
Wed 25 May 2016
SRR1294861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386538
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC266776.90152067843265No Hit
CCCATGTACTCTGCGTTGATACCAC264276.836843984291324No Hit
GAGTACATGGGAAGCAGTGGTATCA135913.516083800299065No Hit
CATGTACTCTGCGTTGATACCACTG114252.955724922258614No Hit
GCGTTGATACCACTGCTTCCCATGT93152.4098536237058195No Hit
ACGCAGAGTACATGGGAAGCAGTGG92662.3971769916541192No Hit
TATCAACGCAGAGTACATGGGAAGC78022.0184302707625124No Hit
GGTATCAACGCAGAGTACATGGGAA77442.0034252777217247No Hit
ACTCTGCGTTGATACCACTGCTTCC75731.9591864189290573No Hit
GTATCAACGCAGAGTACATGGGAAG75361.9496142681961413No Hit
GTATCAACGCAGAGTACTTTTTTTT58501.5134346429070362No Hit
GTGGTATCAACGCAGAGTACATGGG51441.3307876586519307No Hit
GCTTCCCATGTACTCTGCGTTGATA49361.2769766491263472No Hit
GTACTCTGCGTTGATACCACTGCTT47811.236877098758725No Hit
GGTATCAACGCAGAGTACTTTTTTT45621.180220314690923No Hit
GATACCACTGCTTCCCATGTACTCT42141.0901903564461968No Hit
ATACCACTGCTTCCCATGTACTCTG42071.0883794090102397No Hit
GGGAAGCAGTGGTATCAACGCAGAG40901.0581107161520988No Hit
TATCAACGCAGAGTACTTTTTTTTT39811.0299116775064807No Hit
CAGTGGTATCAACGCAGAGTACATG39801.0296529707299154No Hit
CATGGGAAGCAGTGGTATCAACGCA38280.990329540691989No Hit
CTGCTTCCCATGTACTCTGCGTTGA36180.9360011176132746No Hit
GCAGTGGTATCAACGCAGAGTACAT33620.8697721828125566No Hit
ACATGGGAAGCAGTGGTATCAACGC33500.8666677014937729No Hit
GCAGAGTACATGGGAAGCAGTGGTA33460.8656328743875117No Hit
GTTGATACCACTGCTTCCCATGTAC31950.8265681511261506No Hit
TACCACTGCTTCCCATGTACTCTGC31680.8195830681588874No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29660.7673242992926957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28520.7378317267642509No Hit
CCACTGCTTCCCATGTACTCTGCGT27900.7217919066172019No Hit
CTTCCCATGTACTCTGCGTTGATAC27670.7158416507561999No Hit
CCATGTACTCTGCGTTGATACCACT26860.6948864018544102No Hit
AAGCAGTGGTATCAACGCAGAGTAC25900.6700505513041408No Hit
ATGGGAAGCAGTGGTATCAACGCAG24850.6428863397647838No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23920.6188266095442104No Hit
TGATACCACTGCTTCCCATGTACTC20670.5347469071604861No Hit
TTCCCATGTACTCTGCGTTGATACC19290.49904537199447396No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18900.48895580770842706No Hit
ATCAACGCAGAGTACATGGGAAGCA18660.4827468450708598No Hit
GTACATGGTAAGCAGTGGTATCAAC16920.43773186594849667No Hit
ACCATGTACTCTGCGTTGATACCAC13880.3590850058726438No Hit
GGAAGCAGTGGTATCAACGCAGAGT13210.3417516518427684No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10200.26388091209661146No Hit
AAAAAGTACTCTGCGTTGATACCAC10090.2610351375543931No Hit
CTGCGTTGATACCACTGCTTCCCAT10080.2607764307778278No Hit
TCAACGCAGAGTACATGGGAAGCAG9750.2522391071511727No Hit
CTCTGCGTTGATACCACTGCTTCCC9680.2504281597152156No Hit
GTGGTATCAACGCAGAGTACTTTTT9680.2504281597152156No Hit
TGGGAAGCAGTGGTATCAACGCAGA9250.23930376832290745No Hit
GGTAAGCAGTGGTATCAACGCAGAG9050.23412963279160134No Hit
CCCCATGTACTCTGCGTTGATACCA8610.22274653462272792No Hit
TCCATGTACTCTGCGTTGATACCAC8110.20981119579446267No Hit
AGTGGTATCAACGCAGAGTACATGG8100.2095524890178973No Hit
AAAGTACTCTGCGTTGATACCACTG7900.20437835348659122No Hit
TCCCATGTACTCTGCGTTGATACCA7570.19584102985993618No Hit
AACGCAGAGTACATGGGAAGCAGTG7470.1932539620942831No Hit
GAGTACATGGTAAGCAGTGGTATCA7040.18212957070197497No Hit
ATCAACGCAGAGTACTTTTTTTTTT6970.18031862326601783No Hit
GTACATGGGTAAGCAGTGGTATCAA6930.17928379615975662No Hit
ACTGCTTCCCATGTACTCTGCGTTG6390.16531363022523013No Hit
GTACATGGAAAGCAGTGGTATCAAC6340.16402009634240358No Hit
ATGTACTCTGCGTTGATACCACTGC6270.16220914890644644No Hit
TTGATACCACTGCTTCCCATGTACT6180.1598807879173587No Hit
CAACGCAGAGTACATGGGAAGCAGT5890.15237829139696485No Hit
GCTTACCATGTACTCTGCGTTGATA5830.15082605073757302No Hit
ACTCTGCGTTGATACCACTGCTTAC5150.13323398993113225No Hit
GCGTTGATACCACTGCTTACCATGT4870.12599020018730372No Hit
CGTTGATACCACTGCTTCCCATGTA4800.12417925275134657No Hit
GAGTACATGGAAAGCAGTGGTATCA4690.12133347820912821No Hit
ACGCAGAGTACATGGTAAGCAGTGG4620.11952253077317106No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4570.11822899689034455No Hit
CGCAGAGTACATGGGAAGCAGTGGT4560.11797029011377924No Hit
ATGGTAAGCAGTGGTATCAACGCAG4320.1117613274762119No Hit
TATCAACGCAGAGTACATGGTAAGC4120.1065871919449058No Hit
CTGCTTACCATGTACTCTGCGTTGA3930.10167176319016501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1051.2933015E-913.6151441
AAAAAGT1850.012.87918951
AAAAGTA1900.012.5386352
TTGGGAG900.00112037039.48990818
AAAGTAC2855.456968E-128.3590893
AAGTACT2907.2759576E-128.2149684
AACAAAA2907.2759576E-128.2149682
AGTACTC2907.2759576E-128.2149685
TGGGTAA1553.901434E-57.96236856
GAAAAAA3450.07.734981
GGGTAAG1605.8331818E-57.7095537
ACAAAAA4300.07.53484443
TGGGAGG1300.00371471147.30276419
CCATGTA74850.07.29502252
CATGGTA5400.07.2352564
GAAGCAG75250.07.174839
CAGTGGT83800.07.042910613
AGTGGTA83950.07.0416314
TGCGTTG81500.07.03210212
GGAAGCA77050.06.99498
Produced by FastQC (version 0.11.5)