FastQCFastQC Report
Wed 25 May 2016
SRR1294860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689937
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC71790.26688357385321665No Hit
CCCATGTACTCTGCGTTGATACCAC63200.23494974045860556No Hit
GAGTAAGCAGTGGTATCAACGCAGA52030.1934246043680577No Hit
GAGTACATGGGAAGCAGTGGTATCA32560.1210437270463955No Hit
GCTTACTCTGCGTTGATACCACTGC28510.1059876123492855No Hit
GTATCAACGCAGAGTACTTTTTTTT27810.10338532092015539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT550.003065456912.0930084
TTGCGTG1251.8473656E-811.39433516
TACTGCG855.337796E-511.1740377
TAGCGTG600.005869955311.0852585
ATCGTCC1053.4777295E-610.8547798
TAACCCG1002.395562E-510.4518145
TCGTCCG750.002653430710.1311279
TGGACCG856.585755E-410.060575
CGTCCGC856.608356E-410.05682110
GTCTAGC1054.1010826E-59.9537381
TTAGACT2201.8189894E-129.9335424
CTAGCAC2550.09.6874143
TGTACCG900.00111230089.501655
CGGGACT1106.826686E-59.5016494
CTAGGGC1901.0513759E-99.5011193
TGATCGT800.004524689.49810910
GATCTAG1651.064127E-79.2133771
CTTGCGT1554.292615E-79.18915115
TTCGCCT2304.1836756E-119.08514810
ACACCGG1054.5039452E-49.045659
Produced by FastQC (version 0.11.5)