FastQCFastQC Report
Wed 25 May 2016
SRR1294860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689937
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96380.35829835419937345No Hit
GTACATGGGAAGCAGTGGTATCAAC72640.2700434991600175No Hit
CCCATGTACTCTGCGTTGATACCAC63570.23632523735686004No Hit
GGTATCAACGCAGAGTACTTTTTTT59110.21974492339411666No Hit
GAGTAAGCAGTGGTATCAACGCAGA53310.19818308012418134No Hit
TATCAACGCAGAGTACTTTTTTTTT52500.19517185718475935No Hit
GAGTACATGGGAAGCAGTGGTATCA35230.1309696100689347No Hit
GCTTACTCTGCGTTGATACCACTGC28670.10658242181880097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG603.9894317E-412.70548255
TACGTCG657.823338E-411.7281375
ACCGGTC600.005929722511.0701818
ACCGTTC600.005929722511.0701818
ACCGACT700.001506867110.8442588
TAATCGG700.001506867110.84425816
TCGACCG803.8142235E-410.6748179
ATCGACC803.8142235E-410.6748178
TGGACCG1409.2724804E-810.2097625
CGCCTAA856.668673E-410.04688614
ACGGGTC951.5948228E-410.0306435
TACACCG951.5948228E-410.0306435
GTACCGC951.6568728E-49.9927766
TGCTCGG1252.6109246E-69.86827610
TGGACGG1752.4429028E-99.8013725
TTAGACC1304.0789546E-69.5291124
AGGACCG1201.6407841E-59.5291125
ACACCGT1002.776593E-49.4931376
ATACCGT1002.776593E-49.4931376
CTGGACG2355.456968E-129.3263654
Produced by FastQC (version 0.11.5)