FastQCFastQC Report
Wed 25 May 2016
SRR1294859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185389
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC37890.1733787440130796No Hit
CCCATGTACTCTGCGTTGATACCAC33970.15544143399641894No Hit
GTATCAACGCAGAGTACTTTTTTTT27790.1271627156538264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT350.002174236616.28160911
ACGACAG456.756822E-414.7798115
TCCCGTA700.001490678310.8581392
ACCGTCT700.001494675810.8546538
GTCTTAC2200.010.36531
GTCTAGC1752.566594E-99.7729981
TAGACAG2950.09.6623465
TAGAACC1304.2322354E-69.50130754
GTTCTAG2201.6734703E-109.0696371
CCAGGAC3250.09.0623693
GTCTAGG1804.208232E-88.9736621
GTATTAC2651.8189894E-128.9637021
CCGTCTG850.0074507658.9391279
CTTACAC3350.08.7918513
TAGACTG3050.08.7225115
CTTAGAC1201.7622912E-48.7091323
GTAGTAC1201.7622912E-48.7091323
GTGCTAC1752.618999E-78.6871091
GTGTAGA3500.08.6871091
CAGCGTG1107.161563E-48.6343849
Produced by FastQC (version 0.11.5)