FastQCFastQC Report
Wed 25 May 2016
SRR1294859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185389
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95950.4390522694129055No Hit
GGTATCAACGCAGAGTACTTTTTTT63750.2917100799903358No Hit
TATCAACGCAGAGTACTTTTTTTTT55530.2540966390880525No Hit
GTACATGGGAAGCAGTGGTATCAAC40280.18431501210997217No Hit
CCCATGTACTCTGCGTTGATACCAC36500.16701832030819228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27420.12546965322878445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCT350.002188276716.26592611
CCATGCG400.005324384714.2320349
CAGGACG600.005737199411.1192244
CCACGCA700.001507603210.8434559
GTCCTAC1605.83168E-910.1289961
CAGAACG750.00267616510.1205589
TAACCCG856.4020586E-410.0913955
GCGTGAT1151.0416914E-59.90099916
AGGACGG800.00439780629.5307625
CTAGTAC1606.314258E-89.5307623
CTAGGAC1356.5734184E-69.1777723
GAACCTA1356.922828E-69.1403796
GACAATC1151.1253617E-49.0761227
GTCTTAG1909.966243E-99.0314261
CTTATAC2850.09.0291433
ATACCGT950.00183822988.9923576
TATACTG5800.08.8734685
CTAGACA2159.786163E-108.8658264
GTCTAGG2053.8889993E-98.8356241
GTCTAGA2053.8889993E-98.8356241
Produced by FastQC (version 0.11.5)