FastQCFastQC Report
Wed 25 May 2016
SRR1294858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327128
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC37270.2808319920911924No Hit
CCCATGTACTCTGCGTTGATACCAC31800.23961516899650975No Hit
GTATCAACGCAGAGTACTTTTTTTT20250.15258513120060763No Hit
GAGTACATGGGAAGCAGTGGTATCA17420.13126088817355974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT500.001504858113.293526
GTCCAGA1603.6379788E-1211.87459851
TAGGGGT1052.7143506E-711.7632864
CGGGACT752.0711106E-411.4013394
GTCTTAT1104.9676055E-711.2268921
TGCGGGA600.005878007511.082542
CCGTGTG700.001492980510.8555459
TCTCACG1201.4348007E-610.33939419
CCTACAC1752.3646862E-1010.3135473
GCACTGT2500.010.2550016
TTAGGGA1303.8318285E-710.231974
CTAGTAC750.002648699610.13260653
ACTCGCT750.002649530310.13222410
TAAGTGC750.002657850310.1283956
GTTCTAC856.593612E-410.0584831
CCTAAAC1054.1061838E-59.9516682
TCTAGCA1151.0266418E-59.91233352
GTCTTAG1252.5690315E-69.8796651
CTAGACA1356.426344E-79.8530084
GTGTAGA2151.0913936E-119.7206021
Produced by FastQC (version 0.11.5)