FastQCFastQC Report
Wed 25 May 2016
SRR1294858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327128
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73260.5520190968768649No Hit
GGTATCAACGCAGAGTACTTTTTTT45790.3450307732185592No Hit
TATCAACGCAGAGTACTTTTTTTTT40840.307732185591744No Hit
GTACATGGGAAGCAGTGGTATCAAC38810.29243599713064605No Hit
CCCATGTACTCTGCGTTGATACCAC33460.25212338222085584No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19940.15024926005630204No Hit
GAGTACATGGGAAGCAGTGGTATCA18590.1400769179762615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCC456.840094E-414.7552418
CTAGTAC653.2370008E-614.6727073
GTACGAC550.002979380812.13832953
ATGCGGG855.0994135E-511.2232811
TTAGTAC855.113214E-511.2203053
CGATCAG600.00594200311.06643110
TCCTAGA1303.0770025E-811.0053632
ATTAGAC803.6359305E-410.7294163
GCGGGAC803.6359305E-410.7294163
CGGGACT803.6359305E-410.7294164
ATTACAC1355.3645635E-810.5969543
GCTACAC1355.3645635E-810.5969543
GACTGCG909.648988E-510.5406497
GAACTGT1909.094947E-1210.4903116
CTACACC2100.010.445574
TCGAACT1002.4131383E-510.44390119
CTCGAAC1106.150158E-610.34822218
GTGTAAG2400.010.3347721
TATACAG2800.010.2184925
TAAGACT1605.7789293E-910.1333374
Produced by FastQC (version 0.11.5)