FastQCFastQC Report
Wed 25 May 2016
SRR1294857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012830
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC43390.2155671368173169No Hit
CCCATGTACTCTGCGTTGATACCAC38320.19037872050794155No Hit
GTATCAACGCAGAGTACTTTTTTTT20290.10080334653199724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCG400.005274394514.2522055
CTAGGAC1700.013.4131733
TAGGACC1301.9826984E-1012.4253084
ACGGGTC550.003065427312.092785
CGGTGAA550.003072872312.0888649
TCGTGGG1103.8275175E-812.08826212
TCGTCTA550.003084356412.08284514
CTGTCGC1604.5474735E-1111.2793429
ACCGAGC855.3336003E-511.1745818
GGCTCGG951.370352E-510.99293717
CCCCGCG700.001494457710.85476612
CACGGGT951.6413858E-410.0017974
GTCCTAC1451.6142985E-79.8288621
TACTCCG800.0045106559.501475
TCTGTCG1401.0611075E-69.4983938
TGTCGCC1804.1964086E-99.49815710
GTCGCCC1606.658047E-89.4979211
GACAATG2357.2759576E-129.2958377
GTTCTAG1951.6789272E-99.2576121
TCGCCCA1856.722985E-99.241219512
Produced by FastQC (version 0.11.5)