FastQCFastQC Report
Wed 25 May 2016
SRR1294857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012830
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73530.3653065584276864No Hit
GGTATCAACGCAGAGTACTTTTTTT46380.23042184387156392No Hit
GTACATGGGAAGCAGTGGTATCAAC43280.21502064257786302No Hit
TATCAACGCAGAGTACTTTTTTTTT40160.19952007869517047No Hit
CCCATGTACTCTGCGTTGATACCAC38580.1916704341648326No Hit
GAGTACATGGGAAGCAGTGGTATCA21580.10721223352195665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG250.005908284319.0686594
ACCGTCT500.001516272713.2807518
CGTAGCA701.0540874E-412.2596472
GCGGCTC855.3994576E-511.16112715
CCGGGTT700.001508926910.842236517
TAGACAG3000.010.1699525
GATAGTC750.002677948710.1196737
TCCGAAT951.6677396E-49.98602313
TAGGACT3500.09.8067394
GTCCTAA1201.6252005E-59.5364721
TATTCCG800.0043839929.5343295
CCTATAC1909.858923E-109.5343293
GTGCGGC900.0011281529.48672213
TTCGGTT800.0045688319.48672112
CGGGTTC800.00456976759.48648618
GTATTAG3100.09.2288441
GGACCAT1554.307094E-79.1866326
GGCTATG1356.5227014E-69.183271
TTATACT3650.09.14250854
GTATTAA4100.09.0712781
Produced by FastQC (version 0.11.5)