FastQCFastQC Report
Wed 25 May 2016
SRR1294856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868237
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC204291.0934908151374798No Hit
CCCATGTACTCTGCGTTGATACCAC193501.0357358300900796No Hit
GAGTACATGGGAAGCAGTGGTATCA96140.5146028046762803No Hit
CATGTACTCTGCGTTGATACCACTG75470.403963736934875No Hit
GCGTTGATACCACTGCTTCCCATGT67160.35948329896046377No Hit
GTATCAACGCAGAGTACATGGGAAG60260.32255008331384083No Hit
ACGCAGAGTACATGGGAAGCAGTGG58720.3143070177927104No Hit
TATCAACGCAGAGTACATGGGAAGC56170.3006577859233063No Hit
GGTATCAACGCAGAGTACATGGGAA53020.2837969700846306No Hit
ACTCTGCGTTGATACCACTGCTTCC51910.2778555397414782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47210.25269813198218427No Hit
GTGGTATCAACGCAGAGTACATGGG42790.22903946340855041No Hit
GTATCAACGCAGAGTACTTTTTTTT41850.22400798185669163No Hit
GCTTCCCATGTACTCTGCGTTGATA35170.18825234699880153No Hit
GTACTCTGCGTTGATACCACTGCTT33920.18156154706281913No Hit
ATACCACTGCTTCCCATGTACTCTG30330.1623455696466776No Hit
GGTATCAACGCAGAGTACTTTTTTT29750.15924103847638174No Hit
GGTATCAACGCAGAGTACATGGGGG26720.14302253943156035No Hit
CAGTGGTATCAACGCAGAGTACATG26450.14157732664538814No Hit
GATACCACTGCTTCCCATGTACTCT26170.14007858745972807No Hit
GGGAAGCAGTGGTATCAACGCAGAG24970.13365541952118495No Hit
TATCAACGCAGAGTACTTTTTTTTT24680.13210315393603703No Hit
CTGCTTCCCATGTACTCTGCGTTGA24510.13119320514474342No Hit
CATGGGAAGCAGTGGTATCAACGCA23660.12664346118827535No Hit
TACCACTGCTTCCCATGTACTCTGC23220.12428829961080956No Hit
GCAGTGGTATCAACGCAGAGTACAT22550.12070203084512297No Hit
GCAGAGTACATGGGAAGCAGTGGTA21760.11647344528558207No Hit
GTTGATACCACTGCTTCCCATGTAC21510.11513528529838558No Hit
ACATGGGAAGCAGTGGTATCAACGC20620.1103714357439661No Hit
CTTCCCATGTACTCTGCGTTGATAC20100.10758806297059742No Hit
CCACTGCTTCCCATGTACTCTGCGT18710.10014789344178496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATT400.005137468714.3087419
CGTAGCA1155.329639E-912.390642
GTAGCAC1459.822543E-1111.7924713
CCTATAC1053.4690474E-610.856563
CGTTACC1002.4118937E-510.44495614
AGGACTA1106.044209E-610.3639145
CACCGGA1106.073302E-610.35974417
CAGGACT2053.6379788E-1210.1953954
GTACCGT750.002657440310.1289846
GTACAAG1606.171831E-910.0937511
CGAGCGT856.6203275E-410.0545076
GTCGTTA1054.1175208E-59.94971212
ACGATTC1151.029112E-59.9109168
TAGGACA1356.4403866E-79.8521164
TAGACTG1752.572051E-99.7716915
TAACACT1201.6998752E-59.5002544
GTTCTAG1106.836646E-59.51
GTATTAG2106.730261E-119.51
GTAACAC1201.7014956E-59.499493
CGGACTA900.00111592119.4979619
Produced by FastQC (version 0.11.5)