FastQCFastQC Report
Wed 25 May 2016
SRR1294856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868237
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC205851.101840933457586No Hit
CCCATGTACTCTGCGTTGATACCAC201871.0805374264614178No Hit
GTATCAACGCAGAGTACTTTTTTTT160090.8569041294011414No Hit
GGTATCAACGCAGAGTACTTTTTTT116930.6258841892115401No Hit
GAGTACATGGGAAGCAGTGGTATCA100330.5370303660616934No Hit
TATCAACGCAGAGTACTTTTTTTTT97630.5225782381999714No Hit
CATGTACTCTGCGTTGATACCACTG79300.4244643479387251No Hit
GCGTTGATACCACTGCTTCCCATGT69330.3710985276493293No Hit
ACGCAGAGTACATGGGAAGCAGTGG64060.3428901151192274No Hit
GTATCAACGCAGAGTACATGGGAAG59970.32099781772869285No Hit
TATCAACGCAGAGTACATGGGAAGC59210.31692981136761555No Hit
GGTATCAACGCAGAGTACATGGGAA54600.2922541412037124No Hit
ACTCTGCGTTGATACCACTGCTTCC53600.28690150125492647No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46790.2504500232036942No Hit
GTGGTATCAACGCAGAGTACATGGG43230.23139462498601623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39840.21324917555963188No Hit
GCTTCCCATGTACTCTGCGTTGATA36350.19456846213836895No Hit
GTACTCTGCGTTGATACCACTGCTT32600.17449606233042167No Hit
ATACCACTGCTTCCCATGTACTCTG30680.16421899362875267No Hit
CAGTGGTATCAACGCAGAGTACATG28530.1527108177388629No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28260.1512656049526907No Hit
GGGAAGCAGTGGTATCAACGCAGAG27940.1495527601690792No Hit
GGTATCAACGCAGAGTACATGGGGG27660.14805402098341913No Hit
GATACCACTGCTTCCCATGTACTCT26750.14318311863002392No Hit
CATGGGAAGCAGTGGTATCAACGCA25900.13863337467355588No Hit
CTGCTTCCCATGTACTCTGCGTTGA25180.13477947391043No Hit
GCAGAGTACATGGGAAGCAGTGGTA23880.12782104197700828No Hit
GCAGTGGTATCAACGCAGAGTACAT23840.12760693637905685No Hit
TACCACTGCTTCCCATGTACTCTGC23530.1259476179949332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22770.12187961163385588No Hit
ACATGGGAAGCAGTGGTATCAACGC22620.12107671564153798No Hit
GTTGATACCACTGCTTCCCATGTAC22050.11802571087073No Hit
CCATGTACTCTGCGTTGATACCACT19700.10544700699108304No Hit
CCACTGCTTCCCATGTACTCTGCGT19390.10378768860695939No Hit
CTTCCCATGTACTCTGCGTTGATAC19270.10314537181310508No Hit
GCAGAGTACTTTTTTTTTTTTTTTT18810.10068315743666356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAA854.0036884E-612.27775210
GTCTAGC550.002985285812.13555051
GTCTTAG959.948435E-712.0443061
CGAGCGT1052.7414353E-711.7550566
ACGATAA1001.961247E-611.38482514
GTTCGAT600.00593495611.0685811
CAGGACT1900.011.0367554
TTACTCG700.001500584910.84936919
GCCTAAA1002.4346067E-510.43608911
AATCGTA750.002675636410.12065718
TAATATA1909.276846E-1110.0334134
AGGACTC2300.09.9461655
ATTCGCC1054.165936E-59.93913311
GACGATA1054.165936E-59.93913313
ACGAGCG1252.4627407E-69.91301255
GCGTCTC1554.1476596E-89.7933979
AGTACTG1856.0936145E-109.7893575
GTCCTAA1304.044112E-69.5350751
GGTCTAG800.0043809149.5350751
TCAGGAC1201.6345748E-59.5317433
Produced by FastQC (version 0.11.5)