FastQCFastQC Report
Wed 25 May 2016
SRR1294855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438952
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64260.26347381990297475No Hit
CCCATGTACTCTGCGTTGATACCAC59840.24535128202605055No Hit
ATTCCATTCCATTCCATTCCATTCC38220.15670665105340326No Hit
GAGTACATGGGAAGCAGTGGTATCA29820.12226562884386409No Hit
GAATGGAATGGAATGGAATGGAATG28970.11878052540599406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG402.677401E-416.6928719
TTTACGC402.7594157E-416.626983
TAAGGCG500.001498163313.302135
ACGGAAA550.003074130312.08837313
TCGCATC658.065413E-411.68553114
ACGACTG752.0716495E-411.4018265
CGGTCGT700.001493773210.85553410
GACTGTC1951.8189894E-1210.71591957
ACCCCCG803.766748E-410.6892115
GATAGTC803.7789307E-410.6854767
CTCGCAT803.7796484E-410.68525813
CCGGTCG803.7796484E-410.6852589
CGAATTA1002.4153047E-510.44394415
GCCTTAC1553.6579877E-910.4205951
TCGTGGG1106.0637612E-610.36146312
CGTGGGG951.6481771E-49.99790113
GTCTTAG2107.2759576E-129.9535661
GTATTAG4450.09.8213941
GTGTTAG4450.09.6078851
AGGACGG1002.7518455E-49.5015215
Produced by FastQC (version 0.11.5)