FastQCFastQC Report
Wed 25 May 2016
SRR1294855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438952
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88220.36171273563399364No Hit
GTACATGGGAAGCAGTGGTATCAAC66630.2731911083120947No Hit
CCCATGTACTCTGCGTTGATACCAC62970.2581846629207955No Hit
GGTATCAACGCAGAGTACTTTTTTT56910.23333792546962792No Hit
TATCAACGCAGAGTACTTTTTTTTT52330.21455936812204585No Hit
ATTCCATTCCATTCCATTCCATTCC37210.15256552814487534No Hit
GAATGGAATGGAATGGAATGGAATG31560.12939984058726864No Hit
GAGTACATGGGAAGCAGTGGTATCA31420.12882582355044297No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26440.10840721752621617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGG500.00151550313.28183313
AGGACGG600.005727729811.1217755
CATGCGG600.005936473611.0684210
TGACCGA700.0015087810.84253410
CGAATTA803.8202782E-410.67290115
AACCCGT909.598049E-510.5469746
TTAGACT2900.010.5191174
TCGTGTG1106.1480696E-610.3494813
TGCTCGT856.6779513E-410.04528910
TAGGACG951.5882139E-410.0346844
TCTGTCG2107.2759576E-129.9389918
GGAACGA1054.1689498E-59.938786515
CTGTCGC2201.8189894E-129.9184549
GTTAGAC1252.4599158E-69.9142683
GTGCTAC1356.123828E-79.8888741
CCTAGTC1451.5351725E-79.8630942
TAGACTG4150.09.6478035
GTGTTAG4300.09.53571
GCACCGT800.0045474059.4922776
TAATCGG800.0045674479.48721816
Produced by FastQC (version 0.11.5)