FastQCFastQC Report
Wed 25 May 2016
SRR1294854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999644
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89730.2243449667020365No Hit
CCCATGTACTCTGCGTTGATACCAC82900.20726844689177337No Hit
GAGTACATGGGAAGCAGTGGTATCA42020.10505935028217511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1052.7321948E-711.7591129
TAATCGG600.00590198911.0773716
CCGGTAC700.001491969810.8574223
TGGACCG1106.0480725E-610.3641625
TAGGACT5050.09.9713044
TCTATAC5050.09.5943073
AGGGTCG900.00111380169.5004835
TCGTCGT1304.2557713E-69.4977458
CGAGCCT800.00452692539.49774510
CCTACAC4400.09.284333
ATTAGAC3400.09.2208253
ACGATCT1356.886543E-69.14471518
GTATTAG5650.09.0799931
TAGACAG5650.08.9119575
CGCGGAG1606.7850306E-78.90290918
TCCTATA3850.08.8833462
GTCGTCG1401.08934455E-58.8193347
TTAGGAC3400.08.6619873
GTATTAC4500.08.6558871
GTATTAA7800.08.6477671
Produced by FastQC (version 0.11.5)