FastQCFastQC Report
Wed 25 May 2016
SRR1294854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999644
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130020.32507893202495025No Hit
GTACATGGGAAGCAGTGGTATCAAC91650.22914539394006064No Hit
CCCATGTACTCTGCGTTGATACCAC87380.21846944378049646No Hit
GGTATCAACGCAGAGTACTTTTTTT79590.19899271035122126No Hit
TATCAACGCAGAGTACTTTTTTTTT75940.1898668981539357No Hit
GAGTACATGGGAAGCAGTGGTATCA42960.10740955945079111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAG951.3821264E-510.98513413
AATACCG700.001449224410.8953175
ATACCGT856.6466077E-410.0507576
CGAATTA1054.1695246E-59.939054515
TATTCCG1659.66611E-99.8222945
GTCTTAG3600.09.8006821
GTTCTAA4050.09.6535251
CGGATGT800.0045687499.48716214
ACCGCCG1106.9438785E-59.48716111
GTCGTGC900.0011283169.4870428
ATCGCTC800.004569229.4870428
CTAGGAC3150.09.3820793
TAGGACT5650.09.2803034
AGATCCG1356.5553722E-69.1803145
GTATAGG4400.09.1023541
GTAGGAC3950.08.9300233
CTGTCGC3100.08.8749759
TCTATAC4300.08.8682813
CCATCCG1201.79133E-48.6964559
TAGGACC2201.5043042E-98.666734
Produced by FastQC (version 0.11.5)