FastQCFastQC Report
Wed 25 May 2016
SRR1294853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246963
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC147085.955547997068387No Hit
CCCATGTACTCTGCGTTGATACCAC141855.743775383357021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA86363.4968800994480955No Hit
GAGTACATGGGAAGCAGTGGTATCA74953.0348675712556132No Hit
CATGTACTCTGCGTTGATACCACTG61102.4740548179281916No Hit
GCGTTGATACCACTGCTTCCCATGT52252.1157015423363013No Hit
ACGCAGAGTACATGGGAAGCAGTGG51832.0986949462065168No Hit
GGTATCAACGCAGAGTACATGGGAA50002.0245947773553126No Hit
ACTCTGCGTTGATACCACTGCTTCC44981.8213254617088388No Hit
TATCAACGCAGAGTACATGGGAAGC43081.744390860169337No Hit
GTATCAACGCAGAGTACATGGGAAG42711.7294088588169079No Hit
GTGGTATCAACGCAGAGTACATGGG32541.3176062811028373No Hit
GTACTCTGCGTTGATACCACTGCTT30741.244720869118046No Hit
GCTTCCCATGTACTCTGCGTTGATA28851.168191186534015No Hit
GATACCACTGCTTCCCATGTACTCT26261.06331717706701No Hit
ATACCACTGCTTCCCATGTACTCTG24460.9904317650822188No Hit
GGGAAGCAGTGGTATCAACGCAGAG22670.9179512720528986No Hit
CTGCTTCCCATGTACTCTGCGTTGA22480.9102578118989485No Hit
CAGTGGTATCAACGCAGAGTACATG21940.888392188303511No Hit
CATGGGAAGCAGTGGTATCAACGCA21660.8770544575503213No Hit
GTTGATACCACTGCTTCCCATGTAC20340.8236051554281411No Hit
GCAGAGTACATGGGAAGCAGTGGTA19740.7993100180998772No Hit
GCAGTGGTATCAACGCAGAGTACAT18990.7689410964395477No Hit
TACCACTGCTTCCCATGTACTCTGC18410.745455797022226No Hit
ACATGGGAAGCAGTGGTATCAACGC17880.7239950923822597No Hit
CTTCCCATGTACTCTGCGTTGATAC17120.6932212517664589No Hit
CCACTGCTTCCCATGTACTCTGCGT15900.6438211391989893No Hit
CCATGTACTCTGCGTTGATACCACT14850.6013046488745278No Hit
ATGGGAAGCAGTGGTATCAACGCAG14080.5701258893032559No Hit
AAGCAGTGGTATCAACGCAGAGTAC13580.5498799415297028No Hit
GTATCAACGCAGAGTACTTTTTTTT12380.5012896668731753No Hit
TTCCCATGTACTCTGCGTTGATACC12360.5004798289622332No Hit
TGATACCACTGCTTCCCATGTACTC12320.4988601531403489No Hit
GGTATCAACGCAGAGTACTTTTTTT11090.4490551216174083No Hit
ATCAACGCAGAGTACATGGGAAGCA10900.44136166146345807No Hit
AAACAAAAAAAAAAAAAAAAAAAAA8450.3421565173730478No Hit
GTACATGGTAAGCAGTGGTATCAAC8190.33162862453080016No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8110.3283892728870317No Hit
GGAAGCAGTGGTATCAACGCAGAGT8110.3283892728870317No Hit
TATCAACGCAGAGTACTTTTTTTTT8050.3259597591542053No Hit
CTCTGCGTTGATACCACTGCTTCCC6670.2700809432991987No Hit
CTGCGTTGATACCACTGCTTCCCAT6410.25955305045695104No Hit
ACCATGTACTCTGCGTTGATACCAC6330.2563136988131825No Hit
TCAACGCAGAGTACATGGGAAGCAG5780.2340431562622741No Hit
AAAAAGTACTCTGCGTTGATACCAC5770.23363823730680305No Hit
TGGGAAGCAGTGGTATCAACGCAGA5590.22634969610832392No Hit
AGTGGTATCAACGCAGAGTACATGG4880.1976004502698785No Hit
AAAGTACTCTGCGTTGATACCACTG4650.18828731429404405No Hit
ATGTACTCTGCGTTGATACCACTGC4500.18221352996197812No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4470.18099877309556492No Hit
AACGCAGAGTACATGGGAAGCAGTG4400.1781643404072675No Hit
TCCCATGTACTCTGCGTTGATACCA4220.17087579920878837No Hit
TCCATGTACTCTGCGTTGATACCAC4190.16966104234237517No Hit
TTGATACCACTGCTTCCCATGTACT4120.16682660965407775No Hit
CCCCATGTACTCTGCGTTGATACCA4080.16520693383219348No Hit
ACTGCTTCCCATGTACTCTGCGTTG3840.15548887890088797No Hit
GGTAAGCAGTGGTATCAACGCAGAG3790.15346428412353266No Hit
CAACGCAGAGTACATGGGAAGCAGT3510.1421265533703429No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3450.13969703963751656No Hit
GTACATGGAAAGCAGTGGTATCAAC3380.1368626069492191No Hit
GAGTACATGGTAAGCAGTGGTATCA3370.13645768799374805No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3190.12916914679526892No Hit
GCTTACCATGTACTCTGCGTTGATA3060.12390520037414512No Hit
ACACAAAAAAAAAAAAAAAAAAAAA2970.12026092977490556No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA2920.11823633499755025No Hit
GTACATGGGTAAGCAGTGGTATCAA2830.11459206439831068No Hit
GAACAAAAAAAAAAAAAAAAAAAAA2820.11418714544283962No Hit
CGTTGATACCACTGCTTCCCATGTA2780.11256746962095537No Hit
CGCAGAGTACATGGGAAGCAGTGGT2600.10527892842247624No Hit
TGTACTCTGCGTTGATACCACTGCT2570.10406417155606307No Hit
ACTCTGCGTTGATACCACTGCTTAC2530.10244449573417881No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2520.10203957677870776No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA2490.10082481991229456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1750.016.8395371
GTACAAA702.4534529E-816.2963281
GCACTTT400.00526804414.24771913
GAGAAAA802.824599E-511.8827381
GAAAACA1201.2446799E-711.0905541
GAAAAAA7650.09.9411131
TACAAAA1304.159645E-69.5042612
AAAAGTA1401.0650585E-58.8253862
TGGTAAG2502.2919266E-108.3552726
ATAAAAA1502.5322397E-58.2386991
AAAAAGT1502.5322397E-58.2386991
CATGGTA2553.3833203E-108.1997554
AAATCAA1050.0045075188.13825318
ACATGGT2751.8735591E-107.94901853
ATGGTAA2657.403287E-107.890335
AAGTACT1701.4222525E-57.8270394
TAAAAAA1951.3218814E-67.7983682
AGTACTC1605.685741E-57.72221235
AAAGTAC1605.685741E-57.72221233
AGTGGTA47300.06.703093514
Produced by FastQC (version 0.11.5)