##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294853_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246963
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.398310678117774	33.0	31.0	34.0	19.0	34.0
2	30.62674975603633	33.0	31.0	34.0	23.0	34.0
3	30.967812992229604	34.0	31.0	34.0	25.0	34.0
4	33.65408178553063	37.0	35.0	37.0	24.0	37.0
5	34.509112701092874	37.0	35.0	37.0	31.0	37.0
6	34.26400715896713	37.0	35.0	37.0	27.0	37.0
7	34.51907370739747	37.0	35.0	37.0	30.0	37.0
8	34.331272295849985	37.0	36.0	37.0	28.0	37.0
9	35.7508493175091	39.0	38.0	39.0	27.0	39.0
10	36.030125970287045	39.0	38.0	39.0	30.0	39.0
11	35.92469722185105	39.0	38.0	39.0	28.0	39.0
12	36.12967934467916	39.0	38.0	39.0	29.0	39.0
13	35.73981527597251	39.0	38.0	39.0	27.0	39.0
14	37.268412677202654	41.0	39.0	41.0	27.0	41.0
15	37.122840263521255	41.0	38.0	41.0	27.0	41.0
16	37.09362131169446	41.0	38.0	41.0	27.0	41.0
17	37.17618023752546	41.0	38.0	41.0	28.0	41.0
18	37.09619659625126	41.0	38.0	41.0	28.0	41.0
19	37.058798281523956	41.0	38.0	41.0	28.0	41.0
20	37.067609317995	41.0	38.0	41.0	27.0	41.0
21	37.03028793786923	41.0	38.0	41.0	27.0	41.0
22	37.10242020059685	41.0	38.0	41.0	27.0	41.0
23	37.11017439859412	41.0	38.0	41.0	27.0	41.0
24	37.28124050971198	41.0	38.0	41.0	28.0	41.0
25	36.52132910597944	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20637815191577147
1101	2	0.14180433429892148
1101	3	0.04299390141038728
1101	4	0.049757035338821254
1101	5	-0.04348512542760119
1101	6	0.09170757016536868
1101	7	-0.07066230469777679
1101	8	-0.012148930324229923
1101	9	0.16038554637454894
1101	10	0.20944964933228505
1101	11	0.20268385123281263
1101	12	0.22903154538588666
1101	13	-0.05711981376391151
1101	14	0.11306798318092603
1101	15	0.08276735423851278
1101	16	-0.4028956956822256
1101	17	-0.08183883260872449
1101	18	0.0502338356654235
1101	19	0.03642949443915455
1101	20	0.1202767637761113
1101	21	0.15275018095798032
1101	22	0.11578285823603096
1101	23	0.3985657895258683
1101	24	0.32627393610408006
1101	25	0.7225756460116202
1102	1	-0.09320397601737085
1102	2	0.26151779345307347
1102	3	-0.009194186939705418
1102	4	-0.1695798797550836
1102	5	0.30757333074174653
1102	6	-0.0418507712039613
1102	7	0.013945308839446113
1102	8	-0.14329647130784195
1102	9	0.09625825611418293
1102	10	0.07680970810989152
1102	11	0.21200561272429042
1102	12	0.33386692215955094
1102	13	0.1654085418406268
1102	14	0.31598849564820597
1102	15	0.28639962681968
1102	16	0.2781668932877892
1102	17	0.1967578519667441
1102	18	0.14999505162721505
1102	19	-0.1191245304494899
1102	20	-0.20995696906128813
1102	21	0.0705304078028135
1102	22	0.03287428497066003
1102	23	0.09347326071106465
1102	24	-0.0599822056969046
1102	25	-0.13013593042223448
1103	1	-0.2243073298240965
1103	2	-0.1362993600089908
1103	3	0.20968699374165922
1103	4	0.4088818173270852
1103	5	0.11279625504911905
1103	6	-0.08629570661704378
1103	7	-0.2412135440614449
1103	8	-0.005859622148129517
1103	9	0.20303656904777512
1103	10	0.12869281829798496
1103	11	0.49521595841530797
1103	12	-0.012604731766479915
1103	13	-0.10077501169213576
1103	14	0.08933773636522346
1103	15	0.09666725382098917
1103	16	0.22386821743018714
1103	17	0.3127491912800764
1103	18	0.08442864566489305
1103	19	0.3667503020074605
1103	20	0.19870813632513062
1103	21	-0.02313904265599831
1103	22	0.05204415520764627
1103	23	0.0636469163162019
1103	24	-0.06820838512629734
1103	25	0.3310529992170501
1104	1	-0.508396772138358
1104	2	-0.6396135849814684
1104	3	-0.7006049465795137
1104	4	-0.8589895618916188
1104	5	-0.6012237138207084
1104	6	-0.8861438520936744
1104	7	-0.7160246749980104
1104	8	-0.7905526861927754
1104	9	-0.8850162763263612
1104	10	-1.1410691497366443
1104	11	-0.9484346243264312
1104	12	-0.8954565597153064
1104	13	-0.5825942288820656
1104	14	-0.9127654487834107
1104	15	-0.7269537172886871
1104	16	-1.1648793623883478
1104	17	-1.1121777287305576
1104	18	-0.7432012848616623
1104	19	-1.320733088904042
1104	20	-0.7010906564314041
1104	21	-1.1332639430014666
1104	22	-0.5841579631629514
1104	23	-0.8097823384577225
1104	24	-0.797125282738385
1104	25	-0.9521708723140208
1105	1	-0.09303050043405747
1105	2	-0.026885363802541917
1105	3	-0.08196719413894371
1105	4	0.04275423421837132
1105	5	-0.04753118829719227
1105	6	-0.05659619578545261
1105	7	0.1742801167152379
1105	8	0.23123731306201023
1105	9	0.08957944615669078
1105	10	0.27045182798151757
1105	11	-0.07167033488607899
1105	12	0.02672840190627568
1105	13	-0.10551695039634268
1105	14	-0.05811159976336455
1105	15	-0.16264192058252291
1105	16	0.05026334571211066
1105	17	0.5017279274286182
1105	18	0.3823222265773438
1105	19	0.3666504717189554
1105	20	0.39976633738333334
1105	21	0.4255481890501045
1105	22	0.17538447666117918
1105	23	0.33009395634815064
1105	24	0.15820670921332436
1105	25	0.20965936838945964
1106	1	-0.6882685956721524
1106	2	-0.6300599669771465
1106	3	-0.531702643874393
1106	4	-0.8249706335064957
1106	5	-0.37292801369402184
1106	6	-0.3343739735632312
1106	7	-0.8627569203218002
1106	8	-0.5941595123348122
1106	9	-0.733171876594632
1106	10	-0.7930402355105457
1106	11	-0.9552682184839654
1106	12	-0.8462874711095978
1106	13	-0.9547232996026906
1106	14	-1.0845666262183897
1106	15	-0.8240175819581879
1106	16	-1.2671969717482057
1106	17	-0.8950974693967737
1106	18	-0.9351380908829796
1106	19	-0.91377280870433
1106	20	-0.9520855144685214
1106	21	-0.9739227104207941
1106	22	-1.0706472693705678
1106	23	-1.323345197091001
1106	24	-1.2650737140670998
1106	25	-1.2400760813459897
1107	1	0.23267394154945364
1107	2	0.14451859829909353
1107	3	0.2359237865427346
1107	4	0.008608793096186673
1107	5	-0.05651359262843414
1107	6	-0.10969081864752184
1107	7	0.013458165195608274
1107	8	0.20748931256982672
1107	9	0.22696101377081135
1107	10	0.2107742101643666
1107	11	0.2122597746254229
1107	12	0.39414565304445404
1107	13	0.3670436500712384
1107	14	0.09643497338786489
1107	15	0.3715650438569966
1107	16	0.5236237493050737
1107	17	0.2632635494293609
1107	18	0.47928297470087955
1107	19	0.353976668347471
1107	20	0.2101514733498675
1107	21	0.08304788143351516
1107	22	0.10334067205923247
1107	23	-0.12222547271546347
1107	24	0.05521667599072799
1107	25	-0.18691993516659267
1108	1	-0.008252315655873588
1108	2	0.1357926988755196
1108	3	0.25230224013048996
1108	4	0.1625344539985889
1108	5	0.249986109220103
1108	6	0.07828373909448061
1108	7	0.01961996205508143
1108	8	0.06733824916294395
1108	9	0.3345936911709302
1108	10	-0.1298737723440837
1108	11	0.139033775818028
1108	12	0.15900353418140867
1108	13	-0.052077796957185285
1108	14	0.008758567106802673
1108	15	-0.04302450096510313
1108	16	0.36202565747441895
1108	17	0.385366511067204
1108	18	0.21573695999207132
1108	19	0.1533008583693416
1108	20	0.15658359420059043
1108	21	0.15359051709243232
1108	22	0.07835557963228013
1108	23	-0.05041072415652792
1108	24	-0.22677497576836458
1108	25	-0.3047891460590577
1109	1	-0.19568422744011826
1109	2	0.11032438480616946
1109	3	0.06805884966948383
1109	4	-0.2902090028899451
1109	5	-0.4490307633719013
1109	6	-0.6375924399941368
1109	7	-0.6872353587898772
1109	8	-0.7677620564036829
1109	9	-0.9051432558199011
1109	10	-0.8221681791462601
1109	11	-0.603320415758958
1109	12	-0.8271795400845718
1109	13	-0.6532867779589111
1109	14	-0.4465755633568591
1109	15	-0.6425196517555918
1109	16	-0.5627119537916911
1109	17	-0.6293272580307132
1109	18	-0.6020789029015106
1109	19	-0.688973620174572
1109	20	-0.7476279110471822
1109	21	-0.579742816240902
1109	22	-0.8131151258280624
1109	23	-0.7958072964961076
1109	24	-0.8118154965601789
1109	25	-0.5565494755395903
1110	1	-0.3231346841464884
1110	2	-0.14105107590639676
1110	3	-0.5403755288359378
1110	4	-0.23277350264957164
1110	5	-0.03199908771355098
1110	6	-0.5613144633903175
1110	7	-0.22502714341676722
1110	8	-0.3661008203585965
1110	9	-0.5771826767910042
1110	10	-0.03995836129465147
1110	11	-0.3217248813323792
1110	12	-0.0970102115230631
1110	13	-0.33695752204310025
1110	14	-0.5179926885104322
1110	15	-0.2587527589614069
1110	16	-0.2977020718409378
1110	17	-0.14513837923149708
1110	18	-0.2490528893338606
1110	19	-0.31978928173111143
1110	20	-0.13855254114173476
1110	21	-0.2550005481996678
1110	22	-0.24008216818690897
1110	23	-0.14026005008832954
1110	24	-0.03582863688387761
1110	25	0.12925790654469438
1111	1	0.4483852996024318
1111	2	0.2695933809520632
1111	3	0.5638091240511685
1111	4	0.5271548910328221
1111	5	0.5191537232153038
1111	6	-0.26084724486408817
1111	7	-0.1419942857660672
1111	8	0.06584778560351623
1111	9	0.20443075066316396
1111	10	0.2294921490908095
1111	11	-0.02797392912071217
1111	12	0.10645837818797332
1111	13	0.20473665296071175
1111	14	0.3167287578356195
1111	15	0.22943981632679566
1111	16	0.29994406091006454
1111	17	0.36735463788291867
1111	18	0.500731276020872
1111	19	0.6101279393343475
1111	20	0.7356539491330167
1111	21	0.6714887108526923
1111	22	0.6360668338952564
1111	23	0.8360235312432422
1111	24	0.8675663151246553
1111	25	1.027028461620617
1112	1	-1.48254267141823E-4
1112	2	-0.040943217276453225
1112	3	-0.029037830990603197
1112	4	0.24522208267162426
1112	5	0.02202429001740569
1112	6	-0.25207373797832844
1112	7	-0.05655447178358486
1112	8	0.31145204291177464
1112	9	0.09025530449677888
1112	10	-0.1537554867367419
1112	11	-0.18789865914360604
1112	12	-0.4565682419743098
1112	13	-0.43991234683553415
1112	14	-0.2309382324440108
1112	15	-0.4739808925054376
1112	16	-0.1848581156867226
1112	17	-0.5032541140497742
1112	18	-0.2731831223586667
1112	19	-0.4832703563040681
1112	20	-0.3984416725035089
1112	21	-0.288988558333358
1112	22	0.0669574886721449
1112	23	0.15043149931343436
1112	24	0.3038445263839833
1112	25	0.3319317972312277
1113	1	0.19072078331722508
1113	2	0.18869385177667297
1113	3	0.1595535473817975
1113	4	-0.11209542063128453
1113	5	0.15290301213700275
1113	6	0.0780254388361783
1113	7	0.006956949392069589
1113	8	-0.13909405726936086
1113	9	-0.014206157628919414
1113	10	0.05921461674430617
1113	11	0.12940736619162152
1113	12	-0.39791702273915064
1113	13	-0.2031015479809355
1113	14	0.0744235327717746
1113	15	-0.12048937842998697
1113	16	0.016281311730075743
1113	17	-0.08857666287597254
1113	18	0.14049598475109804
1113	19	0.13917624424472308
1113	20	0.15869409631109477
1113	21	0.033279296781209666
1113	22	0.31719511847181536
1113	23	0.42581305206724807
1113	24	0.06133061233722259
1113	25	0.29106544979553917
1114	1	-0.008602804495570382
1114	2	0.02214208090835612
1114	3	0.06536256110448235
1114	4	0.17242192863426453
1114	5	0.2992390145881174
1114	6	0.08719353433521349
1114	7	0.025522931986827757
1114	8	0.026220374232124755
1114	9	-0.3155631064678772
1114	10	0.19272353442588752
1114	11	0.05259215988000676
1114	12	0.3124807524068274
1114	13	0.18581189129221087
1114	14	0.25006090984799556
1114	15	0.12960047597641733
1114	16	0.5350564635987496
1114	17	0.31170851438762526
1114	18	-0.3260634458371072
1114	19	0.2325482297030419
1114	20	-0.04384068176325684
1114	21	0.21761929407085034
1114	22	0.17901966374959954
1114	23	0.2976246936630602
1114	24	0.3435441535435757
1114	25	0.43732485504774843
1115	1	0.3367209365116217
1115	2	0.23557133750308168
1115	3	0.3938997313682684
1115	4	0.30191833151196334
1115	5	0.4215411728614953
1115	6	0.4325172753424056
1115	7	0.023124850452056478
1115	8	0.35955370756545335
1115	9	0.11129540672156679
1115	10	0.4685029109246912
1115	11	0.6257336466905628
1115	12	0.686581471831289
1115	13	0.300919804961282
1115	14	0.5378306940206556
1115	15	0.3574182217797741
1115	16	0.47750250676421757
1115	17	0.17022170383606294
1115	18	0.2542722855777626
1115	19	0.17499427286850988
1115	20	0.09284996571876292
1115	21	0.16006533723631833
1115	22	0.2581563543970802
1115	23	0.4870731349100623
1115	24	0.5042156163733083
1115	25	0.37497473514367385
1116	1	0.05325479988547244
1116	2	0.20901067893946745
1116	3	0.13447890617023717
1116	4	0.15667902173728976
1116	5	-0.08522798394020015
1116	6	-0.22964052897648912
1116	7	0.08751539180870083
1116	8	0.17530013243045062
1116	9	0.06761983373252889
1116	10	0.021891007293561415
1116	11	-0.15471583235700592
1116	12	0.23153817150821965
1116	13	0.30260842961168066
1116	14	0.028575506483647928
1116	15	0.15055583882550394
1116	16	0.4384471984436402
1116	17	0.5270316104340154
1116	18	0.15391638447956524
1116	19	0.3704408495924625
1116	20	0.43622123447392624
1116	21	0.18431586346569873
1116	22	0.2244718105895842
1116	23	0.215321831796075
1116	24	0.3946719554585201
1116	25	0.2260239691061372
1201	1	-0.3172476918643632
1201	2	-0.8587007857818989
1201	3	-0.4955329847539147
1201	4	-0.4154815190993517
1201	5	-0.3782190189850283
1201	6	-0.14294366737883024
1201	7	-0.16365031957585785
1201	8	-0.22526368442259326
1201	9	-0.21521715372188055
1201	10	-0.10625040117972873
1201	11	-0.07487963317734625
1201	12	-0.24810029095520747
1201	13	-0.23349856198451135
1201	14	-0.15122461910588925
1201	15	-0.23272605624043052
1201	16	0.1934674397358762
1201	17	-0.36335143765074207
1201	18	-0.26530549482087196
1201	19	-0.22021742662435884
1201	20	-0.40121379966237924
1201	21	-0.5387592096835263
1201	22	-0.6419891030074822
1201	23	-0.6824830234419466
1201	24	-0.43703138602477054
1201	25	-0.42248565719819453
1202	1	0.045234449151646317
1202	2	-0.06621849747529751
1202	3	-0.059854785234080055
1202	4	-0.14637424736294236
1202	5	-0.5637536101799938
1202	6	-0.16176740473024864
1202	7	-0.3409440022824697
1202	8	-0.28079223670339104
1202	9	-0.2632707085802579
1202	10	-0.36456863722762733
1202	11	-0.054649270695207974
1202	12	-0.3387502843271264
1202	13	-0.0846025049535939
1202	14	-0.09125525554852487
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2210	2	-0.006255926074658191
2210	3	0.005882683866374805
2210	4	-0.12440589408165437
2210	5	0.004337112275770494
2210	6	-0.07282560061589294
2210	7	0.02519987437074178
2210	8	0.08975089696419758
2210	9	-0.21602408483544622
2210	10	-0.24444797189237022
2210	11	-0.3235276696449745
2210	12	-0.10956295977369024
2210	13	0.04360441375093416
2210	14	-0.15901079651747807
2210	15	-0.21246783828409121
2210	16	-0.09898138384350119
2210	17	-0.012746947149878451
2210	18	-0.1150322707771565
2210	19	-0.23301507095177243
2210	20	-0.12845692659122676
2210	21	-0.05642675406473785
2210	22	0.10657399334291995
2210	23	-0.018605223957244732
2210	24	0.0327440889320485
2210	25	-0.04877745481420703
2211	1	0.41561058860904154
2211	2	0.2907211874723501
2211	3	0.18003062564782013
2211	4	0.5633333201240944
2211	5	0.37973742992619464
2211	6	0.7080737146131
2211	7	0.5464850567593729
2211	8	0.4711006509152966
2211	9	0.5232291762888295
2211	10	0.5973907554229569
2211	11	0.41566049917842207
2211	12	0.7804440701948039
2211	13	0.6241366084029494
2211	14	0.6658388402674831
2211	15	0.559513831723983
2211	16	0.2537277577192327
2211	17	0.009345379769690965
2211	18	0.4000431061072689
2211	19	0.4302754750424498
2211	20	0.3702802827716525
2211	21	0.22678984707567906
2211	22	0.4909570485151562
2211	23	0.5034740845021517
2211	24	0.5839066374258124
2211	25	0.4448565181594688
2212	1	0.285237851135264
2212	2	0.10076655996588713
2212	3	0.3473234002001959
2212	4	0.2187828981111153
2212	5	0.4551143143483074
2212	6	0.4613058949321669
2212	7	0.23115842359354133
2212	8	0.419080843138552
2212	9	0.26047121170864784
2212	10	0.42653648406617606
2212	11	0.004368602803339172
2212	12	0.22615563774127878
2212	13	-0.015993654562365123
2212	14	0.12083225393777042
2212	15	0.2572866765305406
2212	16	-0.7527366029189082
2212	17	-0.1942575865180558
2212	18	-0.03736236786453162
2212	19	0.3866715330021506
2212	20	0.5416346273098753
2212	21	0.383849416768804
2212	22	0.6586767148077826
2212	23	0.6934653027292086
2212	24	0.6301207175350996
2212	25	0.7182379886185615
2213	1	0.03746375688946557
2213	2	0.06299720168977885
2213	3	0.22203332205669568
2213	4	-0.04528293186757537
2213	5	0.20389480215318656
2213	6	0.06521306992625142
2213	7	-0.004207430064994355
2213	8	-0.04304323926731968
2213	9	-0.3734738510429523
2213	10	-0.14419522325089673
2213	11	-0.09329893212443352
2213	12	0.025670200848075808
2213	13	0.012149845319235908
2213	14	0.11801435197234866
2213	15	-0.08686440090257008
2213	16	0.005302705629517845
2213	17	0.24721766804031375
2213	18	-0.06381275858203139
2213	19	0.049861208588225736
2213	20	0.11787189939133214
2213	21	0.056842549612753146
2213	22	0.09918109557974475
2213	23	0.019382896856605214
2213	24	0.12963528064189234
2213	25	0.3998387463737103
2214	1	-0.3424487498523092
2214	2	-0.020151357068534992
2214	3	0.23572062354887535
2214	4	0.18028787175200733
2214	5	0.19712905636304612
2214	6	0.13699349780424086
2214	7	0.3214532638883796
2214	8	0.06483714666184426
2214	9	0.2953761519533913
2214	10	-0.17174361421392348
2214	11	0.14906338584764
2214	12	0.2679306431085138
2214	13	0.20967486479547404
2214	14	0.20218766053589832
2214	15	-0.16717242511303
2214	16	-0.14588085043208565
2214	17	-0.2433678176671279
2214	18	-0.21085536660024928
2214	19	-0.10652400145551866
2214	20	-0.2958513582343656
2214	21	-0.06745780395588952
2214	22	0.04938185065854839
2214	23	-0.267061640807448
2214	24	-0.23105268004606927
2214	25	-0.31827115954106944
2215	1	0.8345102980744805
2215	2	0.6536587038382997
2215	3	0.42384897296754787
2215	4	0.7368934283575683
2215	5	0.740474115837138
2215	6	0.6950744752400553
2215	7	0.5729698379856032
2215	8	0.6520363467642696
2215	9	0.8246681328582781
2215	10	0.7652461614855284
2215	11	1.0013993672158747
2215	12	0.8231888112699082
2215	13	0.9068965620171738
2215	14	0.9590480625575921
2215	15	1.0365453915080138
2215	16	0.9137212736861642
2215	17	0.8976080329861489
2215	18	0.9097816250044843
2215	19	0.7665349361195481
2215	20	0.8062940987498024
2215	21	0.9401083003844093
2215	22	0.9148320064312898
2215	23	0.8555446108310605
2215	24	0.8760372657535598
2215	25	0.8399585793338318
2216	1	-0.08145018696695416
2216	2	0.07225859147348856
2216	3	-0.05075775019365025
2216	4	-0.15715591852197264
2216	5	0.1036992200086857
2216	6	-0.06843856375989787
2216	7	-0.0683698782932538
2216	8	0.05074415143677413
2216	9	0.08005813239009285
2216	10	-0.0828974781979781
2216	11	-0.05161692568172782
2216	12	0.1016135970744898
2216	13	-0.12975075293202565
2216	14	0.3352946096805809
2216	15	0.006370757864111454
2216	16	0.009582503144471843
2216	17	-0.10706212947464167
2216	18	0.007609238656804962
2216	19	-0.04834403761518047
2216	20	-0.1365344512759492
2216	21	-0.20474281624090196
2216	22	0.10292016598931752
2216	23	0.26243397831081694
2216	24	-0.16423264388640746
2216	25	-0.10897395496084528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	222.0
3	37.0
4	20.0
5	19.0
6	23.0
7	78.0
8	49.0
9	141.0
10	180.0
11	245.0
12	385.0
13	654.0
14	1145.0
15	2173.0
16	3081.0
17	3252.0
18	3208.0
19	2962.0
20	2675.0
21	2539.0
22	2191.0
23	1927.0
24	1857.0
25	1599.0
26	1411.0
27	1268.0
28	1249.0
29	1127.0
30	1249.0
31	1542.0
32	1899.0
33	2376.0
34	3342.0
35	5200.0
36	10009.0
37	25321.0
38	160308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63420629553287	23.932015048372662	10.47068685535177	21.9630918007427
2	11.41012015349792	30.620482074331285	30.240793775708063	27.72860399646273
3	21.521873174531056	37.39290582202895	24.23573700266113	16.849484000778865
4	13.911717222524198	28.891070313070426	23.819799939965762	33.37741252443961
5	9.93561526571381	45.23078296218462	31.438980554755425	13.394621217346147
6	28.98275043904655	35.07424246725909	16.211261493285527	19.731745600408832
7	17.56452535380946	42.189014768933724	20.473692963562392	19.772766913694422
8	25.947208558231583	38.33685465524163	19.625861236936977	16.090075549589812
9	24.813021188228042	25.078685226645902	17.666050163859957	32.442243421266106
10	17.98420495430632	38.38715303833563	25.776670871648015	17.851971135710027
11	22.667402024396573	34.02665131066701	14.993187126914092	28.31275953802232
12	22.984086816876733	40.472772397683585	23.207941992311063	13.335198793128619
13	28.428329742822022	27.530499060787157	19.46746834467286	24.573702851717968
14	20.33720260547214	37.09228660193545	21.31668816262299	21.253822629969417
15	34.79645184971872	25.687991531163938	18.9879578667294	20.527598752387945
16	15.139151383402416	33.61736816917174	31.609386533423102	19.634093914002744
17	25.186257690607423	36.67400747058609	22.594670008557515	15.545064830248979
18	24.492105273831204	27.931877821355712	27.568878596024447	20.007138308788637
19	22.03906015555934	37.67244945132485	27.327104471317227	12.961385921798584
20	23.715998604654935	29.65513884494634	24.630679743970404	21.998182806428325
21	18.12676444819418	34.55397994613363	28.350423467566603	18.96883213810559
22	22.57240411675493	33.012708737155414	17.82791704852353	26.58697009756612
23	16.550868184417702	32.74724294156537	26.55253113985455	24.149357734162376
24	21.19775294570971	40.64977995903385	20.228761483704798	17.923705611551643
25	21.551769801526344	28.81148058657909	18.88209336533251	30.754656246562057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9195.0
1	9195.0
2	6956.0
3	4717.0
4	4717.0
5	4717.0
6	4285.5
7	3854.0
8	3854.0
9	3854.0
10	3585.0
11	3316.0
12	3316.0
13	3316.0
14	3111.0
15	2906.0
16	2906.0
17	2906.0
18	3226.0
19	3546.0
20	3546.0
21	3546.0
22	4134.0
23	4722.0
24	4722.0
25	4722.0
26	5355.5
27	5989.0
28	5989.0
29	5989.0
30	6603.5
31	7218.0
32	7218.0
33	7218.0
34	7504.5
35	7791.0
36	7791.0
37	7791.0
38	7898.5
39	8006.0
40	8006.0
41	8006.0
42	11023.5
43	14041.0
44	14041.0
45	14041.0
46	49217.5
47	84394.0
48	84394.0
49	84394.0
50	69953.5
51	55513.0
52	55513.0
53	55513.0
54	37050.5
55	18588.0
56	18588.0
57	18588.0
58	12103.0
59	5618.0
60	5618.0
61	5618.0
62	4788.0
63	3958.0
64	3958.0
65	3958.0
66	2981.0
67	2004.0
68	2004.0
69	2004.0
70	1507.5
71	1011.0
72	1011.0
73	1011.0
74	695.0
75	379.0
76	379.0
77	379.0
78	256.0
79	133.0
80	133.0
81	133.0
82	87.5
83	42.0
84	42.0
85	42.0
86	29.5
87	17.0
88	17.0
89	17.0
90	10.5
91	4.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010527892842247625
2	0.1801889351846228
3	0.18261844891744916
4	0.1769495835408543
5	0.19274142280422574
6	0.16439709592125137
7	0.174924988763499
8	0.1498200135242931
9	0.16642169069860668
10	0.17411515085255685
11	0.15022493247976418
12	0.15184460830164842
13	0.19436109862610998
14	0.16399217696578028
15	0.16682660965407775
16	0.1607528253220118
17	0.16034790636654075
18	0.16439709592125137
19	0.14941509456882204
20	0.1737102318970858
21	0.17290039398614368
22	0.14617574292505353
23	0.16763644756501986
24	0.1692561233869041
25	0.6235751914254362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	246963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.69155298566992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68679381426392	19.922822447087217
2	11.203857065547156	5.532812607556598
3	3.4569277948146087	2.560707474398999
4	1.4906771183522196	1.4722853220927832
5	0.7674773282605487	0.947510355802286
6	0.39521802587776117	0.5855128096111564
7	0.3214221289296315	0.5555488069062977
8	0.21646796438118038	0.42759441697744194
9	0.12955279686449434	0.28789737733992543
>10	1.0233031043473984	4.961471961386929
>50	0.10823398219059019	1.8646517899442427
>100	0.12463307040128568	6.816810615355337
>500	0.018038997031765036	3.13650222907885
>1k	0.045917446989947354	26.008349428861816
>5k	0.008199544105347742	13.220198977174718
>10k+	0.003279817642139097	11.699323380425408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	14708	5.955547997068387	No Hit
CCCATGTACTCTGCGTTGATACCAC	14185	5.743775383357021	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8636	3.4968800994480955	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7495	3.0348675712556132	No Hit
CATGTACTCTGCGTTGATACCACTG	6110	2.4740548179281916	No Hit
GCGTTGATACCACTGCTTCCCATGT	5225	2.1157015423363013	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5183	2.0986949462065168	No Hit
GGTATCAACGCAGAGTACATGGGAA	5000	2.0245947773553126	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4498	1.8213254617088388	No Hit
TATCAACGCAGAGTACATGGGAAGC	4308	1.744390860169337	No Hit
GTATCAACGCAGAGTACATGGGAAG	4271	1.7294088588169079	No Hit
GTGGTATCAACGCAGAGTACATGGG	3254	1.3176062811028373	No Hit
GTACTCTGCGTTGATACCACTGCTT	3074	1.244720869118046	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2885	1.168191186534015	No Hit
GATACCACTGCTTCCCATGTACTCT	2626	1.06331717706701	No Hit
ATACCACTGCTTCCCATGTACTCTG	2446	0.9904317650822188	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2267	0.9179512720528986	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2248	0.9102578118989485	No Hit
CAGTGGTATCAACGCAGAGTACATG	2194	0.888392188303511	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2166	0.8770544575503213	No Hit
GTTGATACCACTGCTTCCCATGTAC	2034	0.8236051554281411	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1974	0.7993100180998772	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1899	0.7689410964395477	No Hit
TACCACTGCTTCCCATGTACTCTGC	1841	0.745455797022226	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1788	0.7239950923822597	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1712	0.6932212517664589	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1590	0.6438211391989893	No Hit
CCATGTACTCTGCGTTGATACCACT	1485	0.6013046488745278	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1408	0.5701258893032559	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1358	0.5498799415297028	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1238	0.5012896668731753	No Hit
TTCCCATGTACTCTGCGTTGATACC	1236	0.5004798289622332	No Hit
TGATACCACTGCTTCCCATGTACTC	1232	0.4988601531403489	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1109	0.4490551216174083	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1090	0.44136166146345807	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	845	0.3421565173730478	No Hit
GTACATGGTAAGCAGTGGTATCAAC	819	0.33162862453080016	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	811	0.3283892728870317	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	811	0.3283892728870317	No Hit
TATCAACGCAGAGTACTTTTTTTTT	805	0.3259597591542053	No Hit
CTCTGCGTTGATACCACTGCTTCCC	667	0.2700809432991987	No Hit
CTGCGTTGATACCACTGCTTCCCAT	641	0.25955305045695104	No Hit
ACCATGTACTCTGCGTTGATACCAC	633	0.2563136988131825	No Hit
TCAACGCAGAGTACATGGGAAGCAG	578	0.2340431562622741	No Hit
AAAAAGTACTCTGCGTTGATACCAC	577	0.23363823730680305	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	559	0.22634969610832392	No Hit
AGTGGTATCAACGCAGAGTACATGG	488	0.1976004502698785	No Hit
AAAGTACTCTGCGTTGATACCACTG	465	0.18828731429404405	No Hit
ATGTACTCTGCGTTGATACCACTGC	450	0.18221352996197812	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	447	0.18099877309556492	No Hit
AACGCAGAGTACATGGGAAGCAGTG	440	0.1781643404072675	No Hit
TCCCATGTACTCTGCGTTGATACCA	422	0.17087579920878837	No Hit
TCCATGTACTCTGCGTTGATACCAC	419	0.16966104234237517	No Hit
TTGATACCACTGCTTCCCATGTACT	412	0.16682660965407775	No Hit
CCCCATGTACTCTGCGTTGATACCA	408	0.16520693383219348	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	384	0.15548887890088797	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	379	0.15346428412353266	No Hit
CAACGCAGAGTACATGGGAAGCAGT	351	0.1421265533703429	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	345	0.13969703963751656	No Hit
GTACATGGAAAGCAGTGGTATCAAC	338	0.1368626069492191	No Hit
GAGTACATGGTAAGCAGTGGTATCA	337	0.13645768799374805	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	319	0.12916914679526892	No Hit
GCTTACCATGTACTCTGCGTTGATA	306	0.12390520037414512	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	297	0.12026092977490556	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	292	0.11823633499755025	No Hit
GTACATGGGTAAGCAGTGGTATCAA	283	0.11459206439831068	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	282	0.11418714544283962	No Hit
CGTTGATACCACTGCTTCCCATGTA	278	0.11256746962095537	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	260	0.10527892842247624	No Hit
TGTACTCTGCGTTGATACCACTGCT	257	0.10406417155606307	No Hit
ACTCTGCGTTGATACCACTGCTTAC	253	0.10244449573417881	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	252	0.10203957677870776	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	249	0.10082481991229456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.0491895547106245E-4	0.0	0.0	0.0	0.0
9	4.0491895547106245E-4	0.0	0.0	0.0	0.0
10	4.0491895547106245E-4	0.0	0.0	8.098379109421249E-4	0.0
11	4.0491895547106245E-4	0.0	0.0	8.098379109421249E-4	0.0
12	4.0491895547106245E-4	0.0	0.0	0.0016196758218842498	0.0
13	4.0491895547106245E-4	0.0	0.0	0.0016196758218842498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	175	0.0	16.839537	1
GTACAAA	70	2.4534529E-8	16.296328	1
GCACTTT	40	0.005268044	14.247719	13
GAGAAAA	80	2.824599E-5	11.882738	1
GAAAACA	120	1.2446799E-7	11.090554	1
GAAAAAA	765	0.0	9.941113	1
TACAAAA	130	4.159645E-6	9.504261	2
AAAAGTA	140	1.0650585E-5	8.825386	2
TGGTAAG	250	2.2919266E-10	8.355272	6
ATAAAAA	150	2.5322397E-5	8.238699	1
AAAAAGT	150	2.5322397E-5	8.238699	1
CATGGTA	255	3.3833203E-10	8.199755	4
AAATCAA	105	0.004507518	8.138253	18
ACATGGT	275	1.8735591E-10	7.9490185	3
ATGGTAA	265	7.403287E-10	7.89033	5
AAGTACT	170	1.4222525E-5	7.827039	4
TAAAAAA	195	1.3218814E-6	7.798368	2
AGTACTC	160	5.685741E-5	7.7222123	5
AAAGTAC	160	5.685741E-5	7.7222123	3
AGTGGTA	4730	0.0	6.7030935	14
>>END_MODULE