FastQCFastQC Report
Wed 25 May 2016
SRR1294853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246963
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC153696.223199426634759No Hit
CCCATGTACTCTGCGTTGATACCAC147715.981057891263063No Hit
GAGTACATGGGAAGCAGTGGTATCA81053.2818681340929614No Hit
CATGTACTCTGCGTTGATACCACTG62672.537627093937149No Hit
ACGCAGAGTACATGGGAAGCAGTGG56062.2699756643707762No Hit
GCGTTGATACCACTGCTTCCCATGT53542.1679360875920684No Hit
GTATCAACGCAGAGTACTTTTTTTT50852.059012888570353No Hit
GGTATCAACGCAGAGTACATGGGAA49331.997465207338751No Hit
TATCAACGCAGAGTACATGGGAAGC47041.9047387665358777No Hit
GTATCAACGCAGAGTACATGGGAAG45911.8589829245676477No Hit
ACTCTGCGTTGATACCACTGCTTCC45791.854123897101995No Hit
GGTATCAACGCAGAGTACTTTTTTT43201.7492498876349898No Hit
TATCAACGCAGAGTACTTTTTTTTT37561.5208755967493106No Hit
GTGGTATCAACGCAGAGTACATGGG33691.3641719609820093No Hit
GCTTCCCATGTACTCTGCGTTGATA30141.2204257317897822No Hit
GTACTCTGCGTTGATACCACTGCTT29741.2042289735709397No Hit
GATACCACTGCTTCCCATGTACTCT27381.1086681000797691No Hit
ATACCACTGCTTCCCATGTACTCTG24660.9985301441916401No Hit
GGGAAGCAGTGGTATCAACGCAGAG24230.9811186291063845No Hit
CATGGGAAGCAGTGGTATCAACGCA23750.9616825192437733No Hit
CAGTGGTATCAACGCAGAGTACATG23530.95277430222341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22970.9300988407170304No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22160.8973004053238745No Hit
CTGCTTCCCATGTACTCTGCGTTGA21200.8584281855986524No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21110.8547839149994128No Hit
GCAGAGTACATGGGAAGCAGTGGTA20900.8462806169345206No Hit
ACATGGGAAGCAGTGGTATCAACGC20700.8381822378250994No Hit
GCAGTGGTATCAACGCAGAGTACAT20180.8171264521406041No Hit
GTTGATACCACTGCTTCCCATGTAC19310.7818985030146216No Hit
TACCACTGCTTCCCATGTACTCTGC17950.7268295250705571No Hit
CTTCCCATGTACTCTGCGTTGATAC17640.7142770374509542No Hit
CCACTGCTTCCCATGTACTCTGCGT17580.7118475237181278No Hit
AAGCAGTGGTATCAACGCAGAGTAC16130.6531342751748237No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15570.6304588136684443No Hit
ATGGGAAGCAGTGGTATCAACGCAG15310.6199309208261967No Hit
CCATGTACTCTGCGTTGATACCACT15230.6166915691824282No Hit
TGATACCACTGCTTCCCATGTACTC13130.531658588533505No Hit
TTCCCATGTACTCTGCGTTGATACC12420.5029093426950596No Hit
ATCAACGCAGAGTACATGGGAAGCA10680.43245344444309475No Hit
GTGGTATCAACGCAGAGTACTTTTT9490.38426808874203827No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9000.36442705992395624No Hit
GTACATGGTAAGCAGTGGTATCAAC8680.3514696533488822No Hit
GGAAGCAGTGGTATCAACGCAGAGT8280.3352728951300397No Hit
TCAACGCAGAGTACATGGGAAGCAG6660.26967602434372756No Hit
ACCATGTACTCTGCGTTGATACCAC6490.2627924021007195No Hit
CTGCGTTGATACCACTGCTTCCCAT6470.26198256418977744No Hit
ATCAACGCAGAGTACTTTTTTTTTT6400.25914813150148No Hit
TGGGAAGCAGTGGTATCAACGCAGA6250.25307434716941407No Hit
CTCTGCGTTGATACCACTGCTTCCC5940.24052185954981112No Hit
AAAAAGTACTCTGCGTTGATACCAC5840.2364726699951005No Hit
AGTGGTATCAACGCAGAGTACATGG5130.20772342415665504No Hit
TCCATGTACTCTGCGTTGATACCAC4640.18788239533857298No Hit
AACGCAGAGTACATGGGAAGCAGTG4620.18707255742763085No Hit
CCCCATGTACTCTGCGTTGATACCA4560.18464304369480447No Hit
GGTAAGCAGTGGTATCAACGCAGAG4530.1834282868283913No Hit
AAAGTACTCTGCGTTGATACCACTG4450.1801889351846228No Hit
TCCCATGTACTCTGCGTTGATACCA4320.174924988763499No Hit
ATGTACTCTGCGTTGATACCACTGC4080.16520693383219348No Hit
ACTGCTTCCCATGTACTCTGCGTTG3950.15994298741106966No Hit
TTGATACCACTGCTTCCCATGTACT3830.15508395994541693No Hit
CAACGCAGAGTACATGGGAAGCAGT3760.1522495272571195No Hit
GTACATGGAAAGCAGTGGTATCAAC3410.1380773638156323No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3170.1283593088843268No Hit
CGCAGAGTACATGGGAAGCAGTGGT3150.12754947097338468No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3130.12673963306244254No Hit
GCTTACCATGTACTCTGCGTTGATA3090.1251199572405583No Hit
GAGTACATGGTAAGCAGTGGTATCA3080.12471503828508723No Hit
AACGCAGAGTACTTTTTTTTTTTTT2970.12026092977490556No Hit
GTACATGGGTAAGCAGTGGTATCAA2960.11985601081943449No Hit
CGTTGATACCACTGCTTCCCATGTA2940.11904617290849236No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2910.11783141604207918No Hit
CAACGCAGAGTACTTTTTTTTTTTT2870.11621174022019493No Hit
GAGTACATGGAAAGCAGTGGTATCA2810.11378222648736856No Hit
ACGCAGAGTACATGGTAAGCAGTGG2770.1121625506654843No Hit
GCGTTGATACCACTGCTTACCATGT2530.10244449573417881No Hit
ACTCTGCGTTGATACCACTGCTTAC2500.10122973886776561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA952.8740033E-1015.0420421
AAAGAAA752.080682E-411.38797310
AAGAAAA700.001496844710.8456911
AAAAGTA900.00107854369.5266262
AAAAAGT900.00107854369.5266261
GAAAAAA2252.401066E-108.8915181
AACAAAA2409.276846E-118.7327392
CAAAAAA3551.8189894E-127.51243164
ACAAAAA3602.0008883E-117.14351753
CATGGGT1656.632246E-46.92704734
CCATGTA45200.06.78666732
TGCGTTG49100.06.513487312
CTGCGTT49350.06.48049111
AGTGGTA47900.06.419108414
GAAGCAG44800.06.4184459
TACTCTG50050.06.40881637
CAGTGGT48150.06.3857813
ACATGGA1800.0017788996.3497933
TGATACC50350.06.332934417
TCTGCGT50800.06.29551710
Produced by FastQC (version 0.11.5)