##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294853_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246963
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.174009871924135	33.0	31.0	34.0	30.0	34.0
2	32.24754720342723	33.0	31.0	34.0	30.0	34.0
3	32.3794252580346	34.0	31.0	34.0	30.0	34.0
4	35.706705052983644	37.0	35.0	37.0	35.0	37.0
5	35.82240254613039	37.0	35.0	37.0	35.0	37.0
6	35.71437016881071	37.0	37.0	37.0	35.0	37.0
7	35.82322858079955	37.0	37.0	37.0	35.0	37.0
8	35.82017954106485	37.0	37.0	37.0	35.0	37.0
9	37.568319950761854	39.0	39.0	39.0	37.0	39.0
10	37.652231305904124	39.0	39.0	39.0	35.0	39.0
11	37.66108283427072	39.0	39.0	39.0	37.0	39.0
12	37.7178403242591	39.0	39.0	39.0	37.0	39.0
13	37.55114328867077	39.0	39.0	39.0	35.0	39.0
14	39.038710252143034	41.0	40.0	41.0	36.0	41.0
15	38.95556014463705	41.0	39.0	41.0	36.0	41.0
16	38.81890404635512	41.0	39.0	41.0	36.0	41.0
17	39.0095034478849	41.0	39.0	41.0	36.0	41.0
18	39.01020800686742	41.0	39.0	41.0	36.0	41.0
19	38.92450286075242	41.0	39.0	41.0	35.0	41.0
20	39.02185347602677	41.0	39.0	41.0	36.0	41.0
21	38.946093139458135	41.0	39.0	41.0	36.0	41.0
22	39.04028943606937	41.0	39.0	41.0	35.0	41.0
23	38.97530804209538	41.0	40.0	41.0	35.0	41.0
24	38.98665792041723	41.0	40.0	41.0	35.0	41.0
25	38.30967391876516	41.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.15725441681637875
1101	2	-0.17717087917879581
1101	3	-0.25473463637279536
1101	4	-0.18134580689154944
1101	5	-0.07405806174347163
1101	6	-0.001838475911092985
1101	7	-0.10650325685075757
1101	8	-0.025597558299857326
1101	9	-0.11360251189202586
1101	10	0.029453510939340788
1101	11	-0.036485279307996166
1101	12	0.10414181168664527
1101	13	0.026748342607994857
1101	14	0.1329752417599721
1101	15	-0.08399734614662435
1101	16	-0.03092165723524687
1101	17	0.20887225208041826
1101	18	-0.07729352886028806
1101	19	-0.2089793049571611
1101	20	-0.08407066097259275
1101	21	0.020135916824791877
1101	22	0.10399002867423945
1101	23	-0.03916805974652249
1101	24	0.2113386808603437
1101	25	0.279900453416694
1102	1	-0.3982426153744889
1102	2	-0.35157506708346986
1102	3	-0.3369314495242861
1102	4	-0.37953655860206936
1102	5	-0.2750958539095194
1102	6	-0.24289555448022782
1102	7	-0.20596599276479566
1102	8	0.032169810942924926
1102	9	0.12674280656322878
1102	10	0.0408646327651212
1102	11	-0.09289800833403206
1102	12	0.05431860371493258
1102	13	-0.006222222667297217
1102	14	0.14277916332859775
1102	15	0.006533948338386608
1102	16	0.20880374112081768
1102	17	0.09960559459774743
1102	18	-0.15508202130644122
1102	19	-0.04915471938522131
1102	20	-0.0363138533314995
1102	21	0.03974375996205026
1102	22	-0.1400288903687894
1102	23	-0.19359704445516002
1102	24	-0.2117912268404396
1102	25	-0.2970614788972199
1103	1	-0.20661755829938855
1103	2	-0.1267507111115691
1103	3	-0.08946852993022247
1103	4	0.015261137773109112
1103	5	0.13285489211731516
1103	6	0.13108261705439617
1103	7	0.3221738743790965
1103	8	0.2556243644315046
1103	9	0.44899988755832254
1103	10	0.519860994687555
1103	11	0.3225866513510596
1103	12	0.5185490840295444
1103	13	0.35321100314390463
1103	14	0.5637135748290518
1103	15	0.2738590039405864
1103	16	0.36451198792093464
1103	17	0.4188505830354359
1103	18	0.4154922647024222
1103	19	0.548115985989412
1103	20	0.30607785126249354
1103	21	0.4117114149485701
1103	22	0.408466536258409
1103	23	0.43184298618128736
1103	24	0.49462180543092416
1103	25	0.3552664488714754
1104	1	-0.21064929589477188
1104	2	-0.2245374314335038
1104	3	-0.04842225226422414
1104	4	-0.2489277692539602
1104	5	-0.18824909026756131
1104	6	0.0020594212759519337
1104	7	-0.02362165350518808
1104	8	-0.11144598217644841
1104	9	-0.11107755824859566
1104	10	-0.03732362244922882
1104	11	-0.16534471040437637
1104	12	0.01853010221412177
1104	13	-0.22641771254646414
1104	14	-0.05205651742257089
1104	15	-0.2818905879503859
1104	16	-0.1334189941981947
1104	17	-0.1166416154384251
1104	18	-0.22973760508232033
1104	19	-0.05842894396769083
1104	20	0.019854852894930275
1104	21	0.12143390080711924
1104	22	-0.19378091068742265
1104	23	-0.1549608557381532
1104	24	-0.22974704920633116
1104	25	-0.4013714919811875
1105	1	-0.15849935923779412
1105	2	-0.3569094412703038
1105	3	-0.2402621807238745
1105	4	-0.010944312960639024
1105	5	-0.11731981088757237
1105	6	-0.005884538780684068
1105	7	0.0444460117455705
1105	8	0.048476515774218853
1105	9	-0.14379236561129005
1105	10	-0.2551714501562117
1105	11	-0.0925076882715814
1105	12	-0.08602314318419246
1105	13	0.18329985210068145
1105	14	0.2327262532756933
1105	15	-0.008211476243104698
1105	16	-0.2133057219722474
1105	17	-0.32194007284955717
1105	18	-0.08196206294058328
1105	19	-0.26500171392074634
1105	20	-0.24248958409739174
1105	21	-0.22480650458821572
1105	22	-0.25859945156229713
1105	23	-0.21610550138984053
1105	24	-0.043563279923972686
1105	25	-0.22554616967698848
1106	1	-0.09236179310022408
1106	2	0.016106431745569694
1106	3	-0.14766958813127928
1106	4	-0.31782261983894955
1106	5	-0.39509758866535094
1106	6	-0.06937660227274733
1106	7	-0.20687673957717578
1106	8	-0.11819015089244544
1106	9	-0.10675532857425196
1106	10	-0.22342541841018004
1106	11	-0.1295447253086195
1106	12	-0.10983266699371796
1106	13	-0.11564194684112294
1106	14	-0.27256475201531316
1106	15	-0.21985168788331322
1106	16	-0.2979618066283365
1106	17	-0.05738980829929119
1106	18	-0.17190915288767172
1106	19	-0.22002816894720212
1106	20	-0.5070398486476577
1106	21	-0.2856530654347722
1106	22	-0.20304389600674
1106	23	-0.2822430675274106
1106	24	-0.45361618997687714
1106	25	-0.5615250056558239
1107	1	0.2932048366524498
1107	2	0.23943607367986175
1107	3	0.22171640916169366
1107	4	0.1981734426687396
1107	5	0.1302500180776036
1107	6	-0.09423202247700857
1107	7	0.18201214931170995
1107	8	0.08071473405895091
1107	9	0.09454896575329741
1107	10	0.06198125071742311
1107	11	0.05699397285798824
1107	12	0.1710212766509258
1107	13	0.13555775784695356
1107	14	0.19833471888415488
1107	15	0.060140929318606595
1107	16	0.10034014981315664
1107	17	0.10903076509802645
1107	18	0.11331294628791255
1107	19	0.15618691657021344
1107	20	0.20170876707769736
1107	21	0.2567980235279421
1107	22	0.3045849953430775
1107	23	0.30087493419524236
1107	24	0.4226604188578662
1107	25	0.5222082292401993
1108	1	-0.07709927783771064
1108	2	-0.1414027257635837
1108	3	-0.22390076436245465
1108	4	-0.17272697474330556
1108	5	-0.20002229359005952
1108	6	-0.40397163686778725
1108	7	-0.1718340045344462
1108	8	-0.19519462789692454
1108	9	-0.2746022964212216
1108	10	-0.38683608182083873
1108	11	-0.24598754175143256
1108	12	-0.3177284748895275
1108	13	-0.2311486623478345
1108	14	-0.22645160590217017
1108	15	-0.12204949008111754
1108	16	-0.38892639759292535
1108	17	-0.22267267358216003
1108	18	-0.14411102508954343
1108	19	-0.15218120110023392
1108	20	-0.22828526989307818
1108	21	-0.19102547080718324
1108	22	-0.1775656705285158
1108	23	-0.23466471994905902
1108	24	-0.11645705281774354
1108	25	0.06464512051429949
1109	1	-0.05790172237072255
1109	2	0.1889413688292123
1109	3	0.1999467180342478
1109	4	0.12015199430146595
1109	5	-0.014995058303753694
1109	6	-0.2403473509637024
1109	7	-0.09518992322715292
1109	8	-0.13206190569684395
1109	9	-0.17577142090640763
1109	10	-0.09947059756416365
1109	11	-0.03958392809393274
1109	12	-0.15055695901334332
1109	13	-0.277209509963086
1109	14	0.06419539821633435
1109	15	-0.16520081198891745
1109	16	-0.2210640354300466
1109	17	0.0031277781250267367
1109	18	0.010027409877906734
1109	19	-0.05343003861850093
1109	20	-0.10856957971106596
1109	21	-0.0276137529913143
1109	22	0.004963889721246062
1109	23	0.0010998608932411003
1109	24	-0.15915666753808466
1109	25	-0.21142768405591283
1110	1	-0.3935809061750071
1110	2	-0.2928037302573756
1110	3	-0.29786322801564324
1110	4	-0.07987738447103254
1110	5	-0.013913385316200788
1110	6	-0.22206574207529428
1110	7	-0.4262352733687038
1110	8	-0.5916260315448199
1110	9	-0.4200292062811215
1110	10	-0.7902857249612083
1110	11	-0.565998115370256
1110	12	-0.462666354878948
1110	13	-0.31720470252759014
1110	14	-0.33832049302878886
1110	15	-0.651245895050721
1110	16	-0.474242284455201
1110	17	-0.7031379128102841
1110	18	-0.662799896355736
1110	19	-0.6636271434740095
1110	20	-0.5179627745190416
1110	21	-0.3777820132338263
1110	22	-0.4746579253527301
1110	23	-0.6740911011280204
1110	24	-0.8670782529234771
1110	25	-0.9180405786662433
1111	1	0.15531198905631527
1111	2	0.15403746622833125
1111	3	0.17407826809933624
1111	4	0.11494512327362116
1111	5	-0.0021948336936290502
1111	6	0.06490546304380018
1111	7	-0.05444105265873134
1111	8	-0.09784714779082293
1111	9	0.10570590112835276
1111	10	0.15333064800105944
1111	11	0.0626647255215218
1111	12	0.2509591593153502
1111	13	0.06717140838603086
1111	14	0.24951151144026085
1111	15	0.3647131725436168
1111	16	0.1697448162506987
1111	17	0.18216501746243097
1111	18	0.05049551779286077
1111	19	0.11020354059485271
1111	20	0.2351604797595641
1111	21	0.2934383412428403
1111	22	0.22434161758566518
1111	23	0.17168869674918597
1111	24	0.11981931449310679
1111	25	-0.0430977113664639
1112	1	0.20963102349113427
1112	2	0.2535065012624287
1112	3	0.2525544106328681
1112	4	0.21226273141428464
1112	5	0.30190546235229476
1112	6	0.2211266225370423
1112	7	0.15524815904407063
1112	8	0.0348821080192252
1112	9	0.009994372117056116
1112	10	-0.021498256629001844
1112	11	0.07329001767211452
1112	12	-0.025813820201143756
1112	13	0.14012215928794802
1112	14	0.26808329958164023
1112	15	0.11991195480003825
1112	16	0.22318290429739562
1112	17	-0.11947222439630423
1112	18	-0.11822669044608602
1112	19	-0.009527261366002904
1112	20	0.022678608078095408
1112	21	0.08597247042433764
1112	22	0.10675025685310402
1112	23	0.08947901952314652
1112	24	0.18897060954422784
1112	25	0.37720360825527166
1113	1	0.2527690520306223
1113	2	0.21933563497477593
1113	3	0.27100883054713876
1113	4	0.05892425690792891
1113	5	0.2513605577927933
1113	6	0.09743956454341429
1113	7	0.20903917633873448
1113	8	0.0970185643162651
1113	9	0.17531895350003168
1113	10	-0.07215076713552548
1113	11	0.14441754865365652
1113	12	0.01766496050391453
1113	13	-0.025220356419531242
1113	14	-0.0017744812250413133
1113	15	0.12313415510252668
1113	16	-0.02683353550006018
1113	17	0.04655179564230849
1113	18	0.11666513568661685
1113	19	0.1567837078646761
1113	20	0.050231708623897475
1113	21	-0.02168130146301195
1113	22	0.13032193735909203
1113	23	0.12216133687699227
1113	24	0.1809599445992518
1113	25	0.07913513500431435
1114	1	0.2999529265526206
1114	2	0.16444490382504995
1114	3	0.4473591507823542
1114	4	0.2952678601580061
1114	5	0.15408211519061155
1114	6	0.2727116319018137
1114	7	0.08848696945559453
1114	8	0.07580703231337083
1114	9	0.06171941544005932
1114	10	0.2001404907672395
1114	11	0.1586057067267106
1114	12	0.07480057879060809
1114	13	0.012883803986788678
1114	14	-0.14139349854190186
1114	15	0.13797633541233267
1114	16	0.04964582155483299
1114	17	0.17463409638648386
1114	18	0.2549112955155053
1114	19	0.15466255466439094
1114	20	0.32886285775864366
1114	21	0.39945599017787714
1114	22	0.3947826046974541
1114	23	0.3401923174979018
1114	24	0.4213219547454372
1114	25	0.16610241621619792
1115	1	-0.20844035881907885
1115	2	-0.2216462232732681
1115	3	-0.09460727607616093
1115	4	-0.345019909626167
1115	5	-0.17685313660795288
1115	6	-0.487480214620966
1115	7	-0.5038391696331317
1115	8	-0.48774212835809294
1115	9	-0.5665779466702503
1115	10	-0.8598115476020638
1115	11	-0.63276119974811
1115	12	-0.4168061358376889
1115	13	-0.4547420952937884
1115	14	-0.683359925400417
1115	15	-0.4471555590720797
1115	16	-0.31246449019576517
1115	17	-0.5327999563713135
1115	18	-0.44053234956842147
1115	19	-0.6462966779495218
1115	20	-0.6015927777329608
1115	21	-0.44466871006193287
1115	22	-0.4565363401466698
1115	23	-0.4559707520464613
1115	24	-0.4443854793073214
1115	25	-0.4741853535679752
1116	1	0.4193586244881615
1116	2	0.35141904089680764
1116	3	0.34853356577358596
1116	4	0.3266774513114896
1116	5	0.3659986455360169
1116	6	0.42364418659155945
1116	7	0.31159650138994976
1116	8	0.2198326431205686
1116	9	0.24995415771958562
1116	10	0.5486538561140861
1116	11	0.4094133735516863
1116	12	0.42735960736236933
1116	13	0.4710580958491448
1116	14	0.4333252105983618
1116	15	0.5530555190092201
1116	16	0.34328474904364725
1116	17	0.31032212298232054
1116	18	0.4631925922385207
1116	19	0.5353242844492669
1116	20	0.5143829013814454
1116	21	0.3306973051943274
1116	22	0.5842486357845686
1116	23	0.45904100383000923
1116	24	0.45393351830632866
1116	25	0.4807318862343166
1201	1	-0.15780545690454773
1201	2	-0.11660238948783785
1201	3	-0.060975165371282
1201	4	-0.08718683182610931
1201	5	0.08829212423255939
1201	6	0.02759624879846001
1201	7	0.06179357007673758
1201	8	0.21522992023254517
1201	9	0.003791986891094723
1201	10	0.12829632675418878
1201	11	-0.03825319959086215
1201	12	0.08983772939799195
1201	13	0.007438979518497035
1201	14	0.10726003764553838
1201	15	-0.05630758171626127
1201	16	0.18421357718639086
1201	17	-0.06979331250607146
1201	18	-0.023934480322836293
1201	19	0.10385074509564873
1201	20	-0.18385483693806037
1201	21	-0.2689994094368586
1201	22	-0.1974059395491139
1201	23	-0.2591708149469625
1201	24	-0.4303704618131192
1201	25	-0.34350956632891183
1202	1	-0.28748009199210856
1202	2	-0.14831405342962967
1202	3	-0.33205613100838605
1202	4	-0.2300218810948138
1202	5	0.04929694063483936
1202	6	0.03404757662312363
1202	7	0.3383463811176384
1202	8	0.08042220809726786
1202	9	0.08781390373082587
1202	10	0.06433538821855223
1202	11	0.2632874599763966
1202	12	0.1465814290430245
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2209	23	0.24482245953812054
2209	24	0.1897321602680222
2209	25	0.42450365278662616
2210	1	0.5275905056085506
2210	2	0.5542263429224761
2210	3	0.573003712852902
2210	4	0.695281527980228
2210	5	0.4357821437687832
2210	6	0.5860775195026164
2210	7	0.6379706985345095
2210	8	0.6270054518161103
2210	9	0.7154202889381551
2210	10	0.729544946477283
2210	11	0.6702606314915585
2210	12	0.6965933000540119
2210	13	0.7357438579125599
2210	14	0.6341435847448338
2210	15	0.4824675817934647
2210	16	0.6778996951398994
2210	17	0.6041640475550807
2210	18	0.7924108295670251
2210	19	0.5916476815887535
2210	20	0.7463527000816796
2210	21	0.7547696666978041
2210	22	0.681129692830055
2210	23	0.6435869075253606
2210	24	0.6776308972287595
2210	25	0.5295049584311329
2211	1	0.2826481773871308
2211	2	0.4565227992976162
2211	3	0.23788251098866198
2211	4	0.32450276369246467
2211	5	0.3883168379651565
2211	6	0.2359330145946572
2211	7	0.2322634055931161
2211	8	0.23511074512201446
2211	9	0.28746444755507383
2211	10	0.22661579293756517
2211	11	0.303400423716937
2211	12	0.1997177304963813
2211	13	0.29789398679531587
2211	14	0.37560998409912116
2211	15	0.46918875241340885
2211	16	0.5291898245433941
2211	17	0.4926088623023972
2211	18	0.4287661814560053
2211	19	0.4753667952919898
2211	20	0.39628630593477965
2211	21	0.3965276795600374
2211	22	0.4306935744141427
2211	23	0.31304820364325536
2211	24	0.3608396872930655
2211	25	0.40667578666557347
2212	1	0.0897540953263416
2212	2	0.23281933049730696
2212	3	0.03743854357879428
2212	4	-0.2729261283405151
2212	5	0.012810666330281606
2212	6	-0.2980841586502123
2212	7	-0.15779367593101057
2212	8	-0.12488096098131507
2212	9	-0.07149052146478851
2212	10	-0.2777994873473517
2212	11	-0.33383917232151106
2212	12	-0.06143024237769623
2212	13	-0.189189488835261
2212	14	-0.30301371693030177
2212	15	-0.2637721686970025
2212	16	-0.07550842398077862
2212	17	-0.03183373905390141
2212	18	-0.14953796061357139
2212	19	-0.19623148738352825
2212	20	-0.1764804404183451
2212	21	-0.21025624003794974
2212	22	-0.15089153560195712
2212	23	0.16696585012810772
2212	24	0.22978392303488704
2212	25	0.035767848918581535
2213	1	0.4471658878908684
2213	2	0.16697751188494436
2213	3	0.3370123063067112
2213	4	0.3339647075581311
2213	5	0.4875324281110025
2213	6	0.4672603831447262
2213	7	0.2795731249214626
2213	8	0.2442049968685538
2213	9	0.16260846030173326
2213	10	0.2384922483855405
2213	11	0.20435642322667746
2213	12	0.21729341379089817
2213	13	0.14664996179224943
2213	14	0.24928322836925076
2213	15	0.23961404963120003
2213	16	0.20138004393302822
2213	17	0.12416910252790814
2213	18	0.222993415185627
2213	19	0.17564869258332294
2213	20	0.26427257876233057
2213	21	0.18120717459619584
2213	22	0.16808786292989453
2213	23	0.28478493608596267
2213	24	0.2699094262316635
2213	25	0.2739634444667729
2214	1	0.1274619267234911
2214	2	0.022775438414576854
2214	3	0.042507602045908754
2214	4	-0.05164085365718307
2214	5	0.28330158973382424
2214	6	-0.045066577962728616
2214	7	-0.1349411676416068
2214	8	0.015937483235184402
2214	9	-0.10969333151225413
2214	10	-0.28836698335174304
2214	11	0.08053548477159467
2214	12	-0.11501317217422269
2214	13	0.03374770254853132
2214	14	0.08176360231303903
2214	15	0.23861327058164505
2214	16	-0.16688267554920344
2214	17	0.04655179564230849
2214	18	0.1289501479716293
2214	19	0.07815962924060216
2214	20	-0.011193352801164735
2214	21	0.14539486561315584
2214	22	0.20403201106916669
2214	23	0.05582227053793076
2214	24	0.053195816835128085
2214	25	-0.0805700247008474
2215	1	0.12554753771232896
2215	2	0.1919306075697449
2215	3	-0.032751053857907664
2215	4	0.20527137099697512
2215	5	0.23951451368868248
2215	6	0.41298452524966933
2215	7	0.020721826731062265
2215	8	0.07353986341821184
2215	9	0.06600977967472943
2215	10	0.14666267748437178
2215	11	0.053185328389176334
2215	12	0.09178351849343613
2215	13	0.08655191664306727
2215	14	0.08529363810114177
2215	15	0.1115319822100318
2215	16	0.10032091423503431
2215	17	0.156318227989388
2215	18	0.3140362040254274
2215	19	0.3685797583704087
2215	20	0.3562474017363684
2215	21	0.4069894175302906
2215	22	0.08240124427710782
2215	23	0.17534355134953472
2215	24	0.3266364128563666
2215	25	0.3235367665027198
2216	1	0.5042988837491293
2216	2	0.4379393158426055
2216	3	0.5068926560536084
2216	4	0.5149242105493883
2216	5	0.152396671093058
2216	6	0.21194515074711973
2216	7	0.2574838474249148
2216	8	0.3280637175312293
2216	9	0.3085012352672152
2216	10	0.14418714024011337
2216	11	0.13559204218664433
2216	12	0.1940881675874948
2216	13	-0.017408942947739092
2216	14	0.09639551552985637
2216	15	0.2802606211912604
2216	16	0.31094055934950404
2216	17	0.29182901969311814
2216	18	0.06643566092883901
2216	19	0.014086336393724253
2216	20	0.0894336464107397
2216	21	0.2331399863771466
2216	22	-0.05817766859334483
2216	23	0.12961926650473288
2216	24	0.07481048467621321
2216	25	-0.034895276195904046
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	63.0
10	116.0
11	123.0
12	174.0
13	220.0
14	298.0
15	454.0
16	566.0
17	678.0
18	750.0
19	822.0
20	932.0
21	944.0
22	889.0
23	773.0
24	785.0
25	840.0
26	787.0
27	778.0
28	741.0
29	804.0
30	965.0
31	1308.0
32	1746.0
33	2594.0
34	3977.0
35	6411.0
36	12186.0
37	31373.0
38	173840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.35973740376399	19.673658163203317	11.892563958221118	22.07404047481158
2	12.227063625761305	25.93260010366723	32.89855189840611	28.941784372165348
3	23.733919655980856	31.748480541619596	27.565667731603522	16.95193207079603
4	14.539020015143969	23.148001927414228	27.227155484829712	35.08582257261209
5	10.264022644456013	39.958843050494536	34.73858016657991	15.038554138469548
6	31.68071754692292	29.04136670974433	17.78553805891242	21.492377684420337
7	18.520288329148784	36.959180367700654	23.464404308738963	21.0561269944116
8	28.730214647538293	31.46382251592344	22.1045257791653	17.701437057372967
9	27.56080870413787	16.73853978126278	20.79339820135	34.907253313249356
10	20.069079453519166	31.915031462330234	28.656635433791433	19.359253650359165
11	24.8527107299474	27.138073314626077	17.28072626263853	30.728489692787985
12	25.71275859136794	34.49423597866887	26.538388341573434	13.25461708838976
13	31.620526151690736	20.207075553827902	23.17958560594097	24.99281268854039
14	22.77304697464802	29.911768159602854	26.02940521454631	21.285779651202812
15	37.339601478764024	18.311649923267858	23.675611326393025	20.673137271575097
16	15.355336629373632	27.37819025522042	36.61277195369347	20.653701161712483
17	25.83930256194298	30.295876676884205	27.324557318766928	16.540263442405887
18	24.690743147758976	20.46581876637391	34.043966100185045	20.799471985682068
19	22.045002692711055	30.673420714843925	34.81655146722383	12.465025125221187
20	23.76226398286383	22.429675700408563	32.06917635435268	21.73888396237493
21	17.18071128063718	28.057239343545387	36.089211744269384	18.672837631548045
22	21.954883564611414	26.751997278922584	25.05172881548099	26.241390340985017
23	15.497058263788501	26.165053064629113	34.328624125881205	24.00926454570118
24	20.702864014965925	34.14506743979819	27.76794716574682	17.38412137948907
25	20.98156228350572	22.326612135428693	26.299775960069844	30.392049620995742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2631.0
1	2631.0
2	2242.5
3	1854.0
4	1854.0
5	1854.0
6	2512.0
7	3170.0
8	3170.0
9	3170.0
10	2867.5
11	2565.0
12	2565.0
13	2565.0
14	1877.5
15	1190.0
16	1190.0
17	1190.0
18	1848.5
19	2507.0
20	2507.0
21	2507.0
22	3817.5
23	5128.0
24	5128.0
25	5128.0
26	6781.0
27	8434.0
28	8434.0
29	8434.0
30	9090.5
31	9747.0
32	9747.0
33	9747.0
34	9938.0
35	10129.0
36	10129.0
37	10129.0
38	9116.0
39	8103.0
40	8103.0
41	8103.0
42	11008.5
43	13914.0
44	13914.0
45	13914.0
46	50745.0
47	87576.0
48	87576.0
49	87576.0
50	72563.0
51	57550.0
52	57550.0
53	57550.0
54	38510.0
55	19470.0
56	19470.0
57	19470.0
58	12508.0
59	5546.0
60	5546.0
61	5546.0
62	4735.0
63	3924.0
64	3924.0
65	3924.0
66	2931.5
67	1939.0
68	1939.0
69	1939.0
70	1486.5
71	1034.0
72	1034.0
73	1034.0
74	712.0
75	390.0
76	390.0
77	390.0
78	250.5
79	111.0
80	111.0
81	111.0
82	75.0
83	39.0
84	39.0
85	39.0
86	25.0
87	11.0
88	11.0
89	11.0
90	6.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017816434040726747
2	0.007693460153950186
3	0.0
4	0.0
5	0.43528787713139216
6	0.04940011256746962
7	0.009313135975834438
8	0.0
9	0.0
10	4.0491895547106245E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	8.098379109421249E-4
18	0.0
19	0.0
20	0.0
21	0.0
22	8.098379109421249E-4
23	0.0
24	8.098379109421249E-4
25	0.05344930212218024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	246963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.57190753270733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87609487255192	16.226317302591887
2	12.376734573368763	5.092260784004082
3	3.8106485582127743	2.351769293375931
4	1.6317291605156974	1.3427112563420431
5	0.775514221041236	0.7976903422779932
6	0.4369648656628285	0.5393520486874551
7	0.3228028737329003	0.4648469608807797
8	0.22241905324279107	0.36604673574584046
9	0.15943312666076173	0.29518591853840453
>10	1.0510776498376144	4.258937573644634
>50	0.10628875110717448	1.5718953851386646
>100	0.12990847357543547	6.104153253726267
>500	0.023619722468261	3.4268291201516017
>1k	0.06298592658202933	32.64132683843329
>5k	0.009841551028442082	12.316419868563306
>10k+	0.003936620411376833	12.204257317897822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	15369	6.223199426634759	No Hit
CCCATGTACTCTGCGTTGATACCAC	14771	5.981057891263063	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8105	3.2818681340929614	No Hit
CATGTACTCTGCGTTGATACCACTG	6267	2.537627093937149	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	5606	2.2699756643707762	No Hit
GCGTTGATACCACTGCTTCCCATGT	5354	2.1679360875920684	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5085	2.059012888570353	No Hit
GGTATCAACGCAGAGTACATGGGAA	4933	1.997465207338751	No Hit
TATCAACGCAGAGTACATGGGAAGC	4704	1.9047387665358777	No Hit
GTATCAACGCAGAGTACATGGGAAG	4591	1.8589829245676477	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4579	1.854123897101995	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4320	1.7492498876349898	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3756	1.5208755967493106	No Hit
GTGGTATCAACGCAGAGTACATGGG	3369	1.3641719609820093	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3014	1.2204257317897822	No Hit
GTACTCTGCGTTGATACCACTGCTT	2974	1.2042289735709397	No Hit
GATACCACTGCTTCCCATGTACTCT	2738	1.1086681000797691	No Hit
ATACCACTGCTTCCCATGTACTCTG	2466	0.9985301441916401	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2423	0.9811186291063845	No Hit
CATGGGAAGCAGTGGTATCAACGCA	2375	0.9616825192437733	No Hit
CAGTGGTATCAACGCAGAGTACATG	2353	0.95277430222341	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2297	0.9300988407170304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2216	0.8973004053238745	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	2120	0.8584281855986524	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2111	0.8547839149994128	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	2090	0.8462806169345206	No Hit
ACATGGGAAGCAGTGGTATCAACGC	2070	0.8381822378250994	No Hit
GCAGTGGTATCAACGCAGAGTACAT	2018	0.8171264521406041	No Hit
GTTGATACCACTGCTTCCCATGTAC	1931	0.7818985030146216	No Hit
TACCACTGCTTCCCATGTACTCTGC	1795	0.7268295250705571	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1764	0.7142770374509542	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1758	0.7118475237181278	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1613	0.6531342751748237	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1557	0.6304588136684443	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1531	0.6199309208261967	No Hit
CCATGTACTCTGCGTTGATACCACT	1523	0.6166915691824282	No Hit
TGATACCACTGCTTCCCATGTACTC	1313	0.531658588533505	No Hit
TTCCCATGTACTCTGCGTTGATACC	1242	0.5029093426950596	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1068	0.43245344444309475	No Hit
GTGGTATCAACGCAGAGTACTTTTT	949	0.38426808874203827	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	900	0.36442705992395624	No Hit
GTACATGGTAAGCAGTGGTATCAAC	868	0.3514696533488822	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	828	0.3352728951300397	No Hit
TCAACGCAGAGTACATGGGAAGCAG	666	0.26967602434372756	No Hit
ACCATGTACTCTGCGTTGATACCAC	649	0.2627924021007195	No Hit
CTGCGTTGATACCACTGCTTCCCAT	647	0.26198256418977744	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	640	0.25914813150148	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	625	0.25307434716941407	No Hit
CTCTGCGTTGATACCACTGCTTCCC	594	0.24052185954981112	No Hit
AAAAAGTACTCTGCGTTGATACCAC	584	0.2364726699951005	No Hit
AGTGGTATCAACGCAGAGTACATGG	513	0.20772342415665504	No Hit
TCCATGTACTCTGCGTTGATACCAC	464	0.18788239533857298	No Hit
AACGCAGAGTACATGGGAAGCAGTG	462	0.18707255742763085	No Hit
CCCCATGTACTCTGCGTTGATACCA	456	0.18464304369480447	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	453	0.1834282868283913	No Hit
AAAGTACTCTGCGTTGATACCACTG	445	0.1801889351846228	No Hit
TCCCATGTACTCTGCGTTGATACCA	432	0.174924988763499	No Hit
ATGTACTCTGCGTTGATACCACTGC	408	0.16520693383219348	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	395	0.15994298741106966	No Hit
TTGATACCACTGCTTCCCATGTACT	383	0.15508395994541693	No Hit
CAACGCAGAGTACATGGGAAGCAGT	376	0.1522495272571195	No Hit
GTACATGGAAAGCAGTGGTATCAAC	341	0.1380773638156323	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	317	0.1283593088843268	No Hit
CGCAGAGTACATGGGAAGCAGTGGT	315	0.12754947097338468	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	313	0.12673963306244254	No Hit
GCTTACCATGTACTCTGCGTTGATA	309	0.1251199572405583	No Hit
GAGTACATGGTAAGCAGTGGTATCA	308	0.12471503828508723	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	297	0.12026092977490556	No Hit
GTACATGGGTAAGCAGTGGTATCAA	296	0.11985601081943449	No Hit
CGTTGATACCACTGCTTCCCATGTA	294	0.11904617290849236	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	291	0.11783141604207918	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	287	0.11621174022019493	No Hit
GAGTACATGGAAAGCAGTGGTATCA	281	0.11378222648736856	No Hit
ACGCAGAGTACATGGTAAGCAGTGG	277	0.1121625506654843	No Hit
GCGTTGATACCACTGCTTACCATGT	253	0.10244449573417881	No Hit
ACTCTGCGTTGATACCACTGCTTAC	250	0.10122973886776561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.0491895547106245E-4	0.0	0.0	8.098379109421249E-4	0.0
11	4.0491895547106245E-4	0.0	0.0	8.098379109421249E-4	0.0
12	4.0491895547106245E-4	0.0	0.0	0.0012147568664131875	0.0
13	4.0491895547106245E-4	0.0	0.0	0.0012147568664131875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	95	2.8740033E-10	15.042042	1
AAAGAAA	75	2.080682E-4	11.387973	10
AAGAAAA	70	0.0014968447	10.84569	11
AAAAGTA	90	0.0010785436	9.526626	2
AAAAAGT	90	0.0010785436	9.526626	1
GAAAAAA	225	2.401066E-10	8.891518	1
AACAAAA	240	9.276846E-11	8.732739	2
CAAAAAA	355	1.8189894E-12	7.5124316	4
ACAAAAA	360	2.0008883E-11	7.1435175	3
CATGGGT	165	6.632246E-4	6.9270473	4
CCATGTA	4520	0.0	6.7866673	2
TGCGTTG	4910	0.0	6.5134873	12
CTGCGTT	4935	0.0	6.480491	11
AGTGGTA	4790	0.0	6.4191084	14
GAAGCAG	4480	0.0	6.418445	9
TACTCTG	5005	0.0	6.4088163	7
CAGTGGT	4815	0.0	6.38578	13
ACATGGA	180	0.001778899	6.349793	3
TGATACC	5035	0.0	6.3329344	17
TCTGCGT	5080	0.0	6.295517	10
>>END_MODULE