FastQCFastQC Report
Wed 25 May 2016
SRR1294852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104191
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA75727.267422330143678No Hit
GTACATGGGAAGCAGTGGTATCAAC27352.6249868030828No Hit
CCCATGTACTCTGCGTTGATACCAC25342.4320718680116324No Hit
GAGTACATGGGAAGCAGTGGTATCA15091.4483016767283161No Hit
CATGTACTCTGCGTTGATACCACTG11181.0730293403460953No Hit
GTATCAACGCAGAGTACTTTTTTTT10681.0250405505273967No Hit
ACGCAGAGTACATGGGAAGCAGTGG9730.93386184987187No Hit
GCGTTGATACCACTGCTTCCCATGT9360.8983501454060331No Hit
GGTATCAACGCAGAGTACATGGGAA9240.8868328358495455No Hit
GGTATCAACGCAGAGTACTTTTTTT8630.8282865122707336No Hit
ACTCTGCGTTGATACCACTGCTTCC8350.8014127899722625No Hit
TATCAACGCAGAGTACATGGGAAGC8180.785096601433905No Hit
GTATCAACGCAGAGTACATGGGAAG7670.7361480358188327No Hit
GTGGTATCAACGCAGAGTACATGGG7580.727510053651467No Hit
TATCAACGCAGAGTACTTTTTTTTT6780.6507279899415497No Hit
AAACAAAAAAAAAAAAAAAAAAAAA6580.6315324740140703No Hit
GTACTCTGCGTTGATACCACTGCTT5620.5393939975621695No Hit
GCTTCCCATGTACTCTGCGTTGATA5390.5173191542455683No Hit
GATACCACTGCTTCCCATGTACTCT4910.47124991601961785No Hit
ATACCACTGCTTCCCATGTACTCTG4510.4328588841646591No Hit
CTGCTTCCCATGTACTCTGCGTTGA4160.3992667312915703No Hit
CAGTGGTATCAACGCAGAGTACATG4050.38870919753145666No Hit
CATGGGAAGCAGTGGTATCAACGCA4020.3858298701423347No Hit
GGGAAGCAGTGGTATCAACGCAGAG3890.3733527847894732No Hit
ACATGGGAAGCAGTGGTATCAACGC3730.3579963720474897No Hit
GCAGAGTACATGGGAAGCAGTGGTA3670.3522377172692459No Hit
GTTGATACCACTGCTTCCCATGTAC3660.35127794147287195No Hit
TACCACTGCTTCCCATGTACTCTGC3550.3407204077127583No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3520.33784108032363636No Hit
GCAGTGGTATCAACGCAGAGTACAT3500.3359215287308885No Hit
CCACTGCTTCCCATGTACTCTGCGT3320.31864556439615704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3240.3109673580251653No Hit
CTTCCCATGTACTCTGCGTTGATAC3050.29273161789405994No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2660.2553003618354752No Hit
ACACAAAAAAAAAAAAAAAAAAAAA2660.2553003618354752No Hit
AAGCAGTGGTATCAACGCAGAGTAC2610.2505014828536054No Hit
CCATGTACTCTGCGTTGATACCACT2430.233225518518874No Hit
ATGGGAAGCAGTGGTATCAACGCAG2390.2293864153333781No Hit
TTCCCATGTACTCTGCGTTGATACC2370.2274668637406302No Hit
GGTATCAACGCAGAGTACATGGGGG2080.19963336564578515No Hit
ATCAACGCAGAGTACATGGGAAGCA1850.1775585223291839No Hit
TGATACCACTGCTTCCCATGTACTC1830.17563897073643597No Hit
GTGGTATCAACGCAGAGTACTTTTT1740.16700098856907028No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA1680.16124233379082648No Hit
GGAAGCAGTGGTATCAACGCAGAGT1560.14972502423433884No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1540.14780547264159094No Hit
GTACATGGTAAGCAGTGGTATCAAC1450.13916749047422522No Hit
CTCTGCGTTGATACCACTGCTTCCC1360.1305295083068595No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA1350.12956973251048556No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1300.12477085352861571No Hit
AAAAAAAACAAAAAAAAAAAAAAAA1250.11997197454674588No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1230.11805242295399795No Hit
ACCATGTACTCTGCGTTGATACCAC1220.11709264715762398No Hit
GTACTGGTTCACTATCGGTCAGTCA1140.10941444078663223No Hit
TGGGAAGCAGTGGTATCAACGCAGA1140.10941444078663223No Hit
AAAAAGTACTCTGCGTTGATACCAC1090.10461556180476242No Hit
TCAACGCAGAGTACATGGGAAGCAG1070.10269601021201448No Hit
GGTATCAACGCAGAGTACATGGGCA1050.10077645861926654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1203.6379788E-1214.2507231
CACAAAA2600.09.8658852
ACACAAA2401.8189894E-129.5004821
CCATGTA6850.07.35073762
GAAAAAA3653.6379788E-127.28804061
TGATACC7450.06.882941217
TGCGTTG7600.06.872039312
TTGATAC7650.06.82712516
TACTCTG7750.06.73903277
AGTGGTA8650.06.696537514
CTGCGTT7750.06.61650511
GAAGCAG8350.06.59596069
CCCATGT7250.06.55205631
CAGTGGT8850.06.54520313
CTCTGCG7850.06.53221759
ACTCTGC8000.06.5284388
GGAAGCA8450.06.51790248
ATGTACT7750.06.50023274
TCTGCGT7900.06.490874310
TGTACTC7850.06.4174275
Produced by FastQC (version 0.11.5)